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ID   PHM2       preliminary; circular DNA; SYN; 8451 BP.
XX
AC   X76683;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pHM2 - complete.
XX
KW   cloning vector; beta lactamase gene; neomycin phosphotransferase;
KW   lacZ gene.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8451
RC   pHM2
RA   Kaestner K.H.;
RT   ;
RL   Submitted (09-DEC-1993) to the EMBL/GenBank/DDBJ databases by:
RL   Kaestner K.H., FSPI, DKFZ, Im Neuenheimer Feld 280, 69221 Heidelberg,
RL   GERMANY.
XX
RN   [2]
RP   1-8451
RC   pHM2 from pBR322 & SV40 & neo gene & lacZ gene
RC   pHM3 from pBR322 & neo gene & lacZ gene
RA   Kaestner K.H., Montoliu L., Kern H., Thulke M., Schutz G.;
RT   "Universal beta-galactosidase vectors for promoter analysis and gene
RT   targeting";
RL   Gene 148:67-70(1994).
XX
CC   NCBI gi: 453625
CC   NM (pHM2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. E. coli, lacZ gene
FT                   3. SV40, polyA
FT                   4. mouse, phosphoglycerate kinase promoter
FT                   5. Tn5, neo gene
FT                   6. mouse, phosphoglycerate kinase polyA
FT                   -> PHM2.SEQ 8451bp"
FT   CDS             1..848
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   rep_origin      2077..2210
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   polyA_signal    2077..2210
FT                   /note="PLA human growth hormone gene"
FT   CDS             2266..5690
FT                   /note="ANT E. coli lacZ gene"
FT   polyA_signal    5693..6550
FT                   /note="PLA SV40"
FT   promoter        6557..7064
FT                   /note="POR mouse phosphoglycerate kinase gene"
FT   CDS             7073..7864
FT                   /note="ANT E. coli neomycin phosphotranserase gene
FT                   NPT; neomycin resistance gene (neo)"
FT   polyA_signal    7865..8350
FT                   /note="PLA mouse phosphoglycerate kinase gene"
FT   CDS             8439..8451
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
XX
SQ   Sequence 8451 BP; 1975 A; 2161 C; 2224 G; 2090 T; 1 other;
     atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc
     actaaaggga acaaaagctg ggcccaagct gatctggggt ggcatccctg tgacccctcc
     ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatatcga attccccacc
     gcggtggcgg ccgcgatgtc aatcaagctt acgtaccacg tgaccatgtc gtttactttg
     accaacaaga acgtgatttt cgttgccggt ctgggaggca ttggtctgga caccagcaag
     gagctgctca agcgcgatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
     tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc
     gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc
     aacgtaacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt
     tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt
     atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac
     ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa
     aaccgcctcg cggtgatggt gctgcgttgg agtgacggca gttatctgga agatcaggat
     atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa
     atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag
     gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg
     cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag
     cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg
     agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg
     ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg
     ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct
     ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag
     aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg
     tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg
     ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg
     cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa
     tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc
     cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg
     atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa
     aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg
     atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt
     ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac
     ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc
     tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa
     caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa
     tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag
     ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa
     ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg
     caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt
     ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc
     accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag
     tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc
     gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt
     gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg
     ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg
     tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt
     ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg
     attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg
     ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca
     ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg
     cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc
     tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc
     acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg
     tcagtatcgg cggaattaca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt
     caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag gaaatccatt
     atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt
     tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc
     agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa
     ccgctgtttg gtctgctttc tgacaaactc ggcctcgact ctaggcggcc atcgaattct
     ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     tctgcatcga tggtacaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     gcatgcgctt tsgcagcccc gctggcactt ggcgctacac aagtggcctc tggcctcgca
     cacattccac atccaccggt agcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     cggggcgggc gcgaaggtcc tccggagccc ggcattctgc acgcttcaaa agcgcacgtc
     tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc atatgggatc
     ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     tgacgagttc ttctgaggga tccgctgtaa gtctgcagaa attgatgatc tattaaacaa
     taaagatgtc cactaaaatg gaagtttttt cctgtcatac tttgttaaga agggtgagaa
     cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat
     tagataaatg cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat
     agttggatat cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctccact
     catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt
     tgtgttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
     gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
     cagaagctgg tcgaccggtc gaccagcttg gatccggaga gctcccaacg cgttggagta
     ccgagctcgc ggccgctcta gattgaaaaa ggaagagtat gagtattcaa c
//