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ID   PHM3       preliminary; circular DNA; SYN; 6776 BP.
XX
AC   X76682;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pHM3 - complete.
XX
KW   cloning vector; beta lactamase gene; lacZ gene.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6776
RC   pHM3
RA   Kaestner K.H.;
RT   ;
RL   Submitted (09-DEC-1993) to the EMBL/GenBank/DDBJ databases by:
RL   Kaestner K.H., FSPI, DKFZ, Im Neuenheimer Feld 280, 69221 Heidelberg,
RL   GERMANY.
XX
RN   [2]
RP   1-6776
RC   pHM2 from pBR322 & SV40 & neo gene & lacZ gene
RC   pHM3 from pBR322 & neo gene & lacZ gene
RA   Kaestner K.H., Montoliu L., Kern H., Thulke M., Schutz G.;
RT   "Universal beta-galactosidase vectors for promoter analysis and gene
RT   targeting";
RL   Gene 148:67-70(1994).
XX
CC   NCBI gi: 453622
CC   NM (pHM3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. E. coli, lacZ gene
FT                   3. Tn5, neo gene
FT                   4. mouse, phosphoglycerate kinase polyA
FT                   -> PHM3.SEQ 6776bp"
FT   CDS             1..848
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   rep_origin      849..1789
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   polyA_signal    2077..2210
FT                   /note="PLA human growth hormone gene"
FT   CDS             2266..6201
FT                   /note="ANT E. coli lacZ/neomycin phosphotransferase
FT                   fusion protein"
FT   polyA_signal    6209..6675
FT                   /note="PLA mouse phosphoglycerate kinase gene"
FT   CDS             6764..6776
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
XX
SQ   Sequence 6776 BP; 1545 A; 1774 C; 1863 G; 1594 T; 0 other;
     atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc
     actaaaggga acaaaagctg ggcccaagct gatctggggt ggcatccctg tgacccctcc
     ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatatcga attccccacc
     gcggtggcgg ccgcgatgtc aatcaagctt acgtaccacg tgaccatgtc gtttactttg
     accaacaaga acgtgatttt cgttgccggt ctgggaggca ttggtctgga caccagcaag
     gagctgctca agcgcgatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
     tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc
     gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc
     aacgtaacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt
     tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt
     atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac
     ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa
     aaccgcctcg cggtgatggt gctgcgttgg agtgacggca gttatctgga agatcaggat
     atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa
     atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag
     gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg
     cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag
     cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg
     agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg
     ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg
     ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct
     ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag
     aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg
     tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg
     ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg
     cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa
     tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc
     cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg
     atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa
     aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg
     atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt
     ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac
     ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc
     tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa
     caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa
     tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag
     ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa
     ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg
     caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt
     ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc
     accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag
     tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc
     gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt
     gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg
     ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg
     tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt
     ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg
     attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg
     ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca
     ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg
     cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc
     tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc
     acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg
     tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt
     caaaaaagct cgagcttgga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     cttcttgacg agttcttctg agggatccgc tgtaagtctg cagaaattga tgatctatta
     aacaataaag atgtccacta aaatggaagt tttttcctgt catactttgt taagaagggt
     gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg ggggtggggt
     gggattagat aaatgcctgc tctttactga aggctcttta ctattgcttt atgataatgt
     ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca gctcattcct
     ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc
     cactttgtgt tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga
     gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat
     tccatcagaa gctggtcgac cggtcgacca gcttggatcc ggagagctcc caacgcgttg
     gagtaccgag ctcgcggccg ctctagattg aaaaaggaag agtatgagta ttcaac
//