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ID   PHPH0      preliminary; circular DNA; SYN; 3817 BP.
XX
AC   IG0166; K01193; X01385;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pHph0 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pHph0 from pUC9 & hygromycin resistance gene
RA   Muller A., Piepersberg W.;
RT   ;
RL   Submitted (08-AUG-1989) by:
RL   Muller A., Inst. fur Biochemie, Darmstadt, Germany.
XX
RN   [2]
RC   pHph0 from pUC9 & hygromycin resistance gene
RA   Muller A., Piepersberg W.;
RT   ;
RL   Unpublished (1984).
XX
RN   [3]
RC   pJR225 from E.coli hyg gene/cat gene/amp gene
RC   pJR226 from E.coli cat gene
RC   pJR227 from E.coli hyg gene/cat gene/amp gene
RA   Davies J., O'Connor S.;
RT   "Enzymatic modification of amino-glycoside antibiotics;
RT   3-N-acetyltransferase with broad specificity that determines
RT   resistance to the novel aminoglycoside apramycin";
RL   Antimicrob. Agents Chemother. 14:69-72(1978).
XX
RN   [4]
RC   P1Cm from phage P1 & cat gene
RC   pDZ1 from E.aerogenes, genta gene
RC   pDZ3 from E.coli, genta gene
RC   pDZ4 from S.heidelberg, genta gene
RC   pWP1 from S.heidelberg, genta gene
RC   pWP2 from S.marcescens, genta gene
RC   pWP7a, pWP7b from S.typhimurium, genta gene
RC   pWP8 from K.pneumoniae, genta gene
RC   pWP12a, pWP12b from K.pneumoniae, genta gene
RC   pWP14a, pWP14b from P.aeruginosa, genta gene
RC   pWP21 from C.freundii, genta gene
RC   pWP56 from E.coli, genta gene
RC   pWP57 from P.mirabilis, genta gene
RC   pWP64 from E.coli, genta gene
RA   Piepersberg W., Brau B., Davies J.;
RT   "High frequency transduction of R-factor encoded gentamicin
RT   resistance by bacteriophage P1Cm";
RL   J. Antibiot. 36:588-594(1983).
XX
RN   [5]
RC   P1Km from phage P1 & kan gene
RA   Takano T., Ikeda S.;
RT   "Phage P1 carrying kanalycin resistance gene of R factor";
RL   Virology 70:198-200(1976).
XX
RN   [6]
RC   P1-15 hybrid 2 from phage P1
RA   Iida S., Meyer J., Arber W.;
RT   "Genesis and natural history of IS-mediated transposons";
RL   Cold Spring Harb. Symp. Quant. Biol. 45:27-37(1980).
XX
RN   [7]
RC   pJOE104 from pBR322 & P1Km
RA   Altenbuchner J.;
RT   ;
RL   Unpublished (1983).
XX
CC   old Boehringer vector.
CC   NM (pHph0)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli K-12)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pUC9)(pJHPH)
CC   BR (pHph+1)(pHph-1)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli 1142bp, hyg gene
FT                   -> pJHph
FT                   1. pUC9 remove BamHI-EcoRI 10 bp 254..264, MCS/2655bp
FT                   2. pJHph 1142bp 220..1362, hyg gene
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   \ 1153bp
FT                   -> pHph0 3817bp"
FT   misc_feature    1..255
FT                   /note="from pUC9"
FT   promoter        1..<255
FT                   /note="PRO E. coli lac gene"
FT   CDS             >1..<255
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   fragment"
FT   misc_binding    >150..255
FT                   /note="MCS unique HindIII-PstI-SalI-BamHI,
FT                   from pUC9"
FT   misc_binding    256..260
FT                   /note="SIT BamHI linker"
FT   CDS             261..1402
FT                   /note="ANT E. coli hygromycin B phosphotransferase
FT                   gene (hph) hygromycin resistance gene,
FT                   from pJHph bases 220..1361"
FT   misc_binding    495..495
FT                   /note="SIT unique EcoRI"
FT   misc_binding    589..589
FT                   /note="SIT unique PstI"
FT   misc_binding    604..604
FT                   /note="SIT unique NcoI"
FT   misc_binding    660..660
FT                   /note="SIT unique RsrII"
FT   misc_binding    1032..1032
FT                   /note="SIT unique KspI"
FT   misc_feature    1403..1407
FT                   /note="SIT BamHI linker"
FT   misc_feature    1408..3817
FT                   /note="from pUC9"
FT   misc_binding    1410..1410
FT                   /note="SIT unique AvaI"
FT   misc_binding    1412..1412
FT                   /note="SIT unique SmaI"
FT   misc_binding    1415..1415
FT                   /note="SIT unique EcoRI"
FT   misc_binding    1577..1577
FT                   /note="SIT unique NarI"
FT   misc_binding    1580..1580
FT                   /note="SIT unique BbeI"
FT   misc_binding    1823..1823
FT                   /note="SIT unique DraII"
FT   misc_binding    1826..1826
FT                   /note="SIT unique PssI"
FT   misc_binding    1998..1998
FT                   /note="SIT unique SspI"
FT   CDS             >1998..<3000
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2203..2203
FT                   /note="SIT unique Asp700I"
FT   misc_binding    3691..3691
FT                   /note="SIT unique AflIII"
XX
SQ   Sequence 3817 BP; 893 A; 1001 C; 1022 G; 901 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     ctgcaggtcg acggatcccg cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga gaacgcctca
     gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta cgcttggtgg
     cacagttctc gtccacagtt cgcgggatcc ccgggaattc actggccgtc gttttacaac
     gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     ccgagcgcag cgagtcagtg agcgaggaag cggaaga
//