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ID   PHPHM1     preliminary; circular DNA; SYN; 3831 BP.
XX
AC   IG0167; K01193; X01385;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pHph-1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHph-1
RA   Muller A., Piepersberg W.;
RT   ;
RL   Submitted (08-AUG-1989) by:
RL   Muller A., Inst. fur Biochemie, Darmstadt, Germany.
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RN   [2]
RC   pHph-1, pHph+1 from pUC9 & hygromycin resistance gene
RA   Muller A., Piepersberg W.;
RT   ;
RL   Unpublished (1984).
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CC   old Boehringer vector.
CC   NM (pHph-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli K-12)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pUC12)(pJHPH)
CC   BR (pHph+1)(pHph0)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli 1142bp, hyg gene
FT                   -> pJHph
FT                   1. pUC12 remove EcoRI-BamHI 18bp 231..249, MCS/2662bp
FT                   2. pJHph 1142bp 220..1362, hyg gene
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   \ 1153bp
FT                   -> pHph-1 3831bp"
FT   misc_feature    1..250
FT                   /note="from pUC12"
FT   promoter        1..<250
FT                   /note="PRO E. coli lac promoter"
FT   CDS             >1..<250
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   fragment"
FT   misc_binding    >150..250
FT                   /note="MCS unique HindIII-PstI-SalI-BamHI,
FT                   from pUC9"
FT   misc_feature    251..254
FT                   /note="SIT BamHI linker"
FT   CDS             255..1396
FT                   /note="ANT E. coli hygromycin B phosphotransferase
FT                   gene (hph) hygromycin resistance gene,
FT                   from pJHph bases 220..1361"
FT   misc_binding    489..489
FT                   /note="SIT unique EcoRI"
FT   misc_binding    583..583
FT                   /note="SIT unique PstI"
FT   misc_binding    598..598
FT                   /note="SIT unique NcoI"
FT   misc_binding    654..654
FT                   /note="SIT unique RsrII"
FT   misc_binding    1026..1026
FT                   /note="SIT unique KspI"
FT   misc_feature    1397..1403
FT                   /note="SIT BamHI linker"
FT   misc_feature    1402..3831
FT                   /note="from pUC12"
FT   misc_binding    1405..1405
FT                   /note="SIT unique XbaI"
FT   misc_binding    1411..1411
FT                   /note="SIT unique SalI"
FT   misc_binding    1412..1412
FT                   /note="SIT unique AccI"
FT   misc_binding    1421..1421
FT                   /note="SIT unique PstI"
FT   misc_binding    1426..1426
FT                   /note="SIT unique HindIII"
FT   misc_binding    1571..1571
FT                   /note="SIT unique NarI"
FT   misc_binding    1574..1574
FT                   /note="SIT unique BbeI"
FT   misc_binding    1817..1817
FT                   /note="SIT unique DraII"
FT   misc_binding    1820..1820
FT                   /note="SIT unique PssI"
FT   misc_binding    1992..1992
FT                   /note="SIT unique SspI"
FT   CDS             >1992..<3000
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2197..2197
FT                   /note="SIT unique Asp700I"
FT   misc_binding    3685..3685
FT                   /note="SIT unique AflIII"
XX
SQ   Sequence 3831 BP; 896 A; 1007 C; 1024 G; 904 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
     cgcccgggga tccccctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc ctcagccagc
     aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg gtggcacagt
     tctcgtccac agttcgcggg atcctctaga gtcgacctgc agcccaagct tggcactggc
     cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//