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ID   PHPHP1     preliminary; circular DNA; SYN; 3818 BP.
XX
AC   IG0168; K01193; X01385;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pHph+1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHph+1
RA   Muller A., Piepersberg W.;
RT   ;
RL   Submitted (08-AUG-1989) by:
RL   Muller A., Inst. fur Biochemie, Darmstadt, Germany.
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RN   [2]
RC   pHph-1, pHph+1 from pUC9 & hygromycin resistance gene
RA   Muller A., Piepersberg W.;
RT   ;
RL   Unpublished (1984).
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CC   old Boehringer vector.
CC   NM (pHph+1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli K-12)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pUC8)(pJHPH)
CC   BR (pHph0)(pHph-1)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli 1142bp, hyg gene
FT                   -> pJHph
FT                   1. pUC12 remove EcoRI-BamHI 18bp 231..249, MCS/2662bp
FT                   2. pJHph 1142bp 220..1362, hyg gene
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   \ 1153bp
FT                   -> pHph+1 3818bp"
FT   misc_feature    1..242
FT                   /note="from pUC8"
FT   promoter        1..<242
FT                   /note="PRO E. coli lac gene"
FT   CDS             >1..<242
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   fragment"
FT   misc_binding    >150..242
FT                   /note="MCS unique HindIII-PstI-SalI-BamHI,
FT                   from pUC9"
FT   misc_feature    243..248
FT                   /note="SIT BamHI linker"
FT   CDS             249..1390
FT                   /note="ANT E. coli hygromycin B phosphotransferase
FT                   gene (hph) hygromycin resistance gene,
FT                   from pJHph bases 220..1361"
FT   misc_binding    483..483
FT                   /note="SIT unique EcoRI"
FT   misc_binding    577..577
FT                   /note="SIT unique PstI"
FT   misc_binding    592..592
FT                   /note="SIT unique NcoI"
FT   misc_binding    648..648
FT                   /note="SIT unique RsrII"
FT   misc_binding    1020..1020
FT                   /note="SIT unique KspI"
FT   misc_feature    1391..1395
FT                   /note="SIT BamHI linker"
FT   misc_feature    1396..3818
FT                   /note="from pUC8"
FT   misc_binding    1399..1399
FT                   /note="SIT unique SalI"
FT   misc_binding    1400..1400
FT                   /note="SIT unique AccI"
FT   misc_binding    1409..1409
FT                   /note="SIT unique PstI"
FT   misc_binding    1413..1413
FT                   /note="SIT unique HindIII"
FT   misc_binding    1565..1565
FT                   /note="SIT unique NarI"
FT   misc_binding    1568..1568
FT                   /note="SIT unique BbeI"
FT   misc_binding    1811..1811
FT                   /note="SIT unique DraII"
FT   misc_binding    1814..1814
FT                   /note="SIT unique PssI"
FT   misc_binding    1986..1986
FT                   /note="SIT unique SspI"
FT   CDS             >1986..<3000
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2191..2191
FT                   /note="SIT unique Asp700I"
FT   misc_binding    3679..3679
FT                   /note="SIT unique AflIII"
XX
SQ   Sequence 3818 BP; 893 A; 1002 C; 1022 G; 901 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccggg
     gatccgcgcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     aatagagtag atgccgaccg aacaagagct gatttcgaga acgcctcagc cagcaactcg
     cgcgagccta gcaaggcaaa tgcgagagaa cggccttacg cttggtggca cagttctcgt
     ccacagttcg cgggatccgt cgacctgcag ccaagcttgg cactggccgt cgttttacaa
     cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     accgagcgca gcgagtcagt gagcgaggaa gcggaaga
//