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ID   PHSG299    preliminary; circular DNA; SYN; 2676 BP.
XX
AC   M19415;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHSG299 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2676
RC   pHSG575 from pHSG415r & pHSG439 & pUC8
RC   pHSG576 from pHSG415r & pHSG439 & pUC9
RC   pHSG579 from pHSG575
RC   pHSG587 from pHSG579 & pHSG591
RC   pHSG1327 from pHSG587 & pHSG367
RC   pHSG1330 from pHSG1327
RC   pHSG1337 from pHSG1330
RC   pHSG394 from pHSG1337 & pUC8
RC   pHSG395 from pHSG1337 & pUC9
RC   pHSG396 from pHSG1337 & pUC19
RC   pHSG397 from pHSG1337 & pUC19
RC   pHSG398 from pHSG1337 & pUC18
RC   pHSG399 from pHSG1337 & pUC19
RC   pHSG1339 from pHSG1330 & pHSG429
RC   pHSG298 from pHSG1339 & pUC18
RC   pHSG299 from pHSG1339 & pUC19
RA   Takeshita S., Sato M., Toba M., Masahashi W., Hashimoto-Gotoh T.;
RT   "High-copy-number and low-copy-number plasmid vectors for lacZ
RT   alpha-complementation and chloramphenicol- or kanamycin-resistance
RT   selection";
RL   Gene 61:63-74(1987).
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RN   [2]
RC   pHSG124 from pHSG1 & pDMS630
RC   pHSG401 from pHSG124
RC   pHSG408 from pHSG401 & pML21
RC   pHSG410 from pHSG408
RC   pHSG412 from pHSG410
RC   pHSG415 from pHSG412 & pACYC184
RC   pHSG415s, pHSG415r from pHSG415
RC   pHSG422 from pHSG415 & pHC79
RC   [pHSG418 from pSC101]
RC   pKT550 from pHSG415 & xylE gene
RA   Hashimoto-Gotoh T., Franklin F.C., Nordheim A., Timmis K.N.;
RT   "Specific-purpose plasmid cloning vectors, I. low-copy-number,
RT   temperature-sensitive, mobilization-defective pSC101-derived
RT   containment vectors";
RL   Gene 16:227-235(1981).
XX
RN   [3]
RC   pDMS630 from ColE1
RC   pDMS6641 from pDMS630
RC   pHSG101 or pSC105 from pSC101 & pML21
RC   pHSG100 from pHSG101
RC   pHSG124 from pDMS630 & Tn3 & pHSG1 or pHS1
RC   pHSG401, pHSG402 from pHSG124
RC   pHSG209, pHSG217, pHSG200 series from pHSG124
RC   [pHSG306 from pHSG series]
RA   Hashimoto-Gotoh T., Inselburg J.;
RT   "Isolation and characterization of replication-deficient mutants
RT   of ColE1 plasmids";
RL   J. Bacteriol. 139:597-607(1979).
XX
RN   [4]
RC   pFCO12 from ColE1
RC   from pDMS6641
RC   from pFCO12
RC   from pHSG209
RC   from pHSG217
RC   from pHSG306
RA   Hashimoto-Gotoh T., Timmis K.N.;
RT   "Incompatibility properties of ColE1 and pMB9 derivative plasmids:
RT   random replication of multicopy replicons";
RL   Cell 23:229-238(1981).
XX
CC   NCBI gi: 208472
CC   NM (pHSG299)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP (high)(low)
CC   FN (cloning)(expression)
CC   SE (color blue/white)
CC   PA ()
CC   BR (pHSG399)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSC101 9263bp, ori/tet gene
FT                   mutagenesis
FT                   -> pHSG1 9263bp [temperature sensitive, tet]
FT                   1. R1
FT                   -> R1drd-19
FT                   1. ColE1 6646bp
FT                   transposition
FT                   2. R1drd-19, Tn3 amp gene
FT                   -> pDMS630
FT                   1. pHSG1 EcoRI 9263bp 9262..9262
FT                   2. pDMS630 EcoRI 6646bp 6645..6645
FT                   -> pHSG124 15909bp
FT                   1. pHSG124 remove BamHI-BamHI, pSC101 ori
FT                   \ pSC101 376 to R1drd-19 Tn3 amp gene
FT                   -> pHSG401
FT                   1. pSC101 EcoRI 9263bp 9262..9262
FT                   2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   -> pSC105 16163bp
FT                   1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT                   2. pVH51 EcoRI 3847bp 3846..3846
FT                   -> pML21 10747bp
FT                   1. pHSG401
FT                   2. pML21 10747bp
FT                   -> pHSG408
FT                   1. pHSG408
FT                   -> pHSG410
FT                   1. pHSG410
FT                   -> pHSG412
FT                   1. pHSG412
FT                   2. pACYC184 4245bp
FT                   -> pHSG415
FT                   1. pHSG415
FT                   mutagenesis to temperature resistance
FT                   -> pHSG415r
FT                   1. pHSG415
FT                   -> pHSG415s
FT                   1. pHSG415s
FT                   -> pHSG415h
FT                   1. pHSG415h
FT                   -> pHSG413h
FT                   1. pHSG413h
FT                   2. pHSG262 2800bp
FT                   -> pHSG439
FT                   1. pHSG415r HaeII-HaeII, ori
FT                   2. pHSG439 HhaI-HaeII, Tn9 cat gene
FT                   \ pACYC184 cat gene
FT                   \ pBR328 HhaI-HhaI 1364bp 3170..4534, cat gene
FT                   3. pUC8 HaeII-HaeII 424bp 5..429, MCS/lacZ'
FT                   -> pHSG575 3610bp [cat gene]
FT                   1. pHSG575 remove TaqI-HindIII, MCS
FT                   fill in:fill in
FT                   -> pHSG579 [cat gene]
FT                   1. pNO1523 4799bp, lambda cos
FT                   -> pHSG591
FT                   1. pHSG579 remove PstI-EcoRI, MCS
FT                   nuclease:
FT                   2. pHSG591 HaeIII-EcoRI, MCS
FT                   -> pHSG587
FT                   1. pUC19 remove HindIII-HaeII 237bp 448..685,
FT                   \ lacZ gene 5' end 2449bp
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pNO1523, strep gene
FT                   -> pHSG1311
FT                   1. pHSG1311 AhaII-EcoRI 2221bp 397..2618,strep gene to
FT                   \ lacZ gene 3' end
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pHSG367 2221bp [EcoRI, HindIII, no PvuII]
FT                   1. pHSG587 HaeII-HindIII
FT                   nuclease:
FT                   2. pHSG367 PstI-HindIII
FT                   nuclease:
FT                   -> pHSG1327
FT                   1. pHSG1327 remove HgaI-BamHI, amp/MCS
FT                   fill in:
FT                   -> pHSG1330
FT                   1. pHSG415s
FT                   -> pHSG418
FT                   1. pHSG418
FT                   -> pHSG425
FT                   1. pHSG415
FT                   2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT                   -> pHSG422
FT                   1. pHSG425
FT                   2. pHSG422
FT                   -> pHSG429
FT                   1. pHSG1330 SalI-HindIII, ori
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pHSG429 HaeII-HaeII 1430bp, kan gene/#V00359
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pHSG1339 2231bp
FT                   1. pHSG1339 TaqI 2231bp 2055..2055
FT                   T4 DNA polymerase
FT                   2. pUC19 HaeII-HaeII 445bp 240..685, MCS/lacZ' gene
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pHSG299 2676bp"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2676 BP; 671 A; 625 C; 689 G; 691 T; 0 other;
     gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat
     ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg
     tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct
     gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
     tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
     agccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
     aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     gcggctttgt tgaataaatc gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
     tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcgta
     atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggcgaacttt tgctgagttg
     aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
     aaaatcagta accgtcagtg ccgataagtt caaagttaaa cctggtgttg ataccaacat
     tgaaacgctg atcgaaaacg cgctgaaaaa cgctgctgaa tgtgcgagct tcttccgctt
     cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     ctacggggtc tgacgctcag tggaacgatc cgtcga
//