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ID   PHSG399    preliminary; circular DNA; SYN; 2227 BP.
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AC   M19087;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHSG399 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2227
RC   pHSG575 from pHSG415r & pHSG439 & pUC8
RC   pHSG576 from pHSG415r & pHSG439 & pUC9
RC   pHSG579 from pHSG575
RC   pHSG587 from pHSG579 & pHSG591
RC   pHSG1327 from pHSG587 & pHSG367
RC   pHSG1330 from pHSG1327
RC   pHSG1337 from pHSG1330
RC   pHSG394 from pHSG1337 & pUC8
RC   pHSG395 from pHSG1337 & pUC9
RC   pHSG396 from pHSG1337 & pUC19
RC   pHSG397 from pHSG1337 & pUC19
RC   pHSG398 from pHSG1337 & pUC18
RC   pHSG399 from pHSG1337 & pUC19
RC   pHSG1339 from pHSG1330 & pHSG429
RC   pHSG298 from pHSG1339 & pUC18
RC   pHSG299 from pHSG1339 & pUC19
RA   Takeshita S., Sato M., Toba M., Masahashi W., Hashimoto-Gotoh T.;
RT   "High-copy-number and low-copy-number plasmid vectors for lacZ
RT   alpha-complementation and chloramphenicol- or kanamycin-resistance
RT   selection";
RL   Gene 61:63-74(1987).
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CC   NCBI gi: 493617
CC   NM (pHSG399)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP (high)(low)
CC   FN (cloning)(expression)
CC   SE (color blue/white)
CC   PA ()
CC   BR (pHSG299)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSC101
FT                   -> pHSG415
FT                   1. pHSG415
FT                   mutagenesis to temperature resistance
FT                   -> pHSG415r
FT                   1. pHSG415r HaeII-HaeII, ori
FT                   2. pHSG439 HhaI-HaeII, Tn9 cat gene
FT                   \ pACYC184 cat gene
FT                   \ pBR328 HhaI-HhaI 1364bp 3170..4534, cat gene
FT                   3. pUC8 HaeII-HaeII 424bp 5..429, MCS/lacZ'
FT                   -> pHSG575 3610bp [cat gene]
FT                   1. pHSG575 remove TaqI-HindIII, MCS
FT                   fill in:fill in
FT                   -> pHSG579 [cat gene]
FT                   1. pHSG579 remove PstI-EcoRI, MCS
FT                   nuclease:
FT                   2. pHSG591 HaeIII-EcoRI, MCS
FT                   -> pHSG587
FT                   1. pHSG587 HaeII-HindIII
FT                   nuclease:
FT                   2. pHSG367 PstI-HindIII
FT                   nuclease:
FT                   -> pHSG1327
FT                   1. pHSG1327 remove HgaI-BamHI, amp/MCS
FT                   fill in:
FT                   -> pHSG1330 1782bp
FT                   1. pHSG1330 SalI 1782bp
FT                   fill in
FT                   -> pHSG1337 1782bp
FT                   1. pHSG1337 HindIII 1782bp
FT                   fill in
FT                   2. pUC19 HaeII-HaeII 445bp 240..685, MCS/lacZ' gene
FT                   nuclease:nuclease
FT                   -> pHSG339 2227bp"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2227 BP; 555 A; 566 C; 552 G; 554 T; 0 other;
     acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
     atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
     gaataagtga taataagcgg atgaatggca gaaattcagc ttggcccagt gccaagctcc
     aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct
     gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac
     aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     gcagcctgaa tggcgaatga gcttcttccg cttcctcgct cactgactcg ctgcgctcgg
     tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaact
     ccgtcga
//