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ID   PHSG664    preliminary; circular DNA; SYN; 2706 BP.
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AC   M12787;
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DT   09-MAR-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHSG664 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2706
RC   pHSG1311 from pUC9 & pNO1523, strep gene
RC   pHSG367 from pHSG1311
RC   plasmid from pHSG660 & pHSG591
RC   pHSG664 from plasmid & pHSG367
RA   Hashimoto-Gotoh T., Kume A., Masahashi W., Takeshita S., Fukuda A.;
RT   "Improved vector, pHSG664, for direct streptomycin-resistance
RT   selection: cDNA cloning with G:C-tailing procedure and subcloning
RT   for double-digest DNA fragments";
RL   Gene 41:125-128(1986).
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RN   [2]
RC   pHSG664
RA   Kikuno R.;
RT   ;
RL   Submitted (12-AUG-1986) to GenBank on computer-readable media by:
RL   Kikuno R., .
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CC   NCBI gi: 208391
CC   NM (pHSG664)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC9)(pNO1523)(pHSG684)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 remove HindIII-HaeII 237bp 448..685,
FT                   \ lacZ gene 5' end 2449bp
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pNO1523, strep gene
FT                   -> pHSG1311
FT                   1. pHSG1311 AhaII-EcoRI 2221bp 397..2618,strep gene to
FT                   \ lacZ gene 3' end
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pHSG367 2221bp [EcoRI, HindIII, no PvuII]
FT                   1. pHSG660 SstII
FT                   T4 DNA polymerase
FT                   SstII-BamHI, strep gene
FT                   2. pHSG591 PstI
FT                   fill in
FT                   BglII
FT                   fill in
FT                   remove small SmaI-BamHI
FT                   -> plasmid
FT                   1. plasmid EcoRI-HindIII, strep gene
FT                   2. pHSG367 large EcoRI-HindIII
FT                   -> pHSG664 2706bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-HpaI-PvuII-SphI-PstI-SmaI-
FT                   BamHI-SalI-XbaI-HindIII [1]"
FT   CDS             232..606
FT                   /note="GEN E. coli ribosomal protein S12 gene"
FT   CDS             complement(1712..2572)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
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SQ   Sequence 2706 BP; 684 A; 681 C; 646 G; 695 T; 0 other;
     aattcccggt ttgactggtc aaatttcgaa cgacatcatt ctcattgact atactgattt
     cgtcagactt acggttaagc accccagcca gatggcctgg tgatgatggc gggatcgttg
     tatatttctt gacacctttt cggcatcgcc ctaaaattcg gcgtcctcat attgtgtgag
     gacgttttat tacgtgttta cgaagcaaaa gctaaaacca ggagctattt aatggcaaca
     gttaaccagc tggtacgcaa accacgtgct cgcaaagttg cgaaaagcaa cgtgcctgcg
     ctggaagcat gcccgcaaaa acgtggcgta tgtactcgtg tatatactac cactcctaaa
     aaaccgaact ccgcgctgcg taaagtatgc cgtgttcgtc tgactaacgg tttcgaagtg
     acttcctaca tcggtggtga aggtcacaac ctgcaggagc actccgtgat cctgatccgt
     ggcggtcgtg ttaaagacct cccgggtgtt cgttaccaca ccgtacgtgg tgcgcttgac
     tgctccggcg ttaaagaccg taagcaggct cgttccaagt atggcgtgaa gcgtcctaag
     gcttaatggt tctccgttaa gtaaggccaa acgttttaac ttaaatgtca aactaaactc
     gtagagtttt ggacaatcct gaattaacaa cggagtattt ccatgccacg tcgtcgcgtc
     attggtcagc gtaaaattct gccggatccg tcgaccgatc tctagaagct tcttccgctt
     cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     ctgacg
//