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ID PHSV106 preliminary; circular DNA; SYN; 7940 BP.
XX
AC IG1081; V00470; J02224; V00467;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pHSV-106 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC from pHSV-106
RA McKnight S.L., Gavis E.R.;
RT "Expression of the herpes thymidine kinase gene in Xenopus laevis
RT oocytes: an assay for the study of deletion mutants constructed
RT in vitro";
RL Nucleic Acids Res. 8:5931-5948(1980).
XX
RN [2]
RC pTK1 from HSV tk gene
RC ptk/delta3'-1.13 from HSV 1 tk gene
RC from pHSV-106
RA McKnight S.L.;
RT "The nucleotide sequence and transcript map of the herpes simplex
RT virus thymidine kinase gene";
RL Nucleic Acids Res. 8:5949-5964(1980).
XX
RN [3]
RC from pX1 & M13mp2
RA Wagner M.J., Sharp J.A., Summers W.C.;
RT "Nucleotide sequence of the thymidine kinase gene of herpes simplex
RT virus type 1";
RL Proc. Natl. Acad. Sci. U.S.A. 78:1441-1445(1981).
XX
RN [4]
RC pHSV-106 from pBR322 & HSV 1 tk gene
RA McKnight S.L., Croce C., Kingsbury R.;
RT ;
RL Carnegie Institute of Washington Year Book 78:56-61(1979).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pHSV-106)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (BRL)
CC HO (E.coli HB101)
CC CP ()
CC FN (transformation marker for eukaryotic cells)
CC FN (expression and regulation of tk activity)
CC SE ()
CC PA (pBR322)(HSV-1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 BamHI 4361bp 376..376
FT 2. HSV-1 BamHI-BamHI 3579bp 45056..48635,
FT \ Q frag tk gene/.28 to .32
FT \ [tk gene CDS = #X14112 46672..47802]
FT \ tk gene #X14112 MboI 46720..47856
FT \ tk gene #X14112 BglII = 47856
FT \ tk gene #X14112 PvuII = 46068 48108
FT \ tk gene #X14112 BamHI =40779 41546 44394 45056 48635
FT \ tk gene #M14884 BglII/Sau3AI/MboI-Sau3AI/MboI
FT \ 1558bp 2..1560,
FT \ tk gene #M14884 PvuII 1794
FT \ [tk gene CDS = #M14884 59..1189]
FT -> pHSV-106 7940bp [7760bp]"
FT - 1..375
FT /note="pBR322 1..375 375bp
FT BamHI = G^GATCC"
FT - 376..3954
FT /note="HSV-1 45056..48634 3579bp
FT BamHI = G^GATCC"
FT - 3955..7940
FT /note="pBR322 376..4361 3986bp"
FT misc_binding 23..23
FT /note="SIT unique ClaI"
FT promoter complement(27..33)
FT /note="PRO E. coli promoter P1"
FT misc_binding 29..29
FT /note="SIT unique HindIII"
FT promoter 43..49
FT /note="PRO E. coli promoter P2"
FT misc_binding 375..375
FT /note="SIT double BamHI"
FT CDS 375..3775
FT /note="from HSV1"
FT misc_binding >375..<600
FT /note="SIT unique BglII"
FT CDS >600..<2200
FT /note="GEN Herpes simplex virus type 1 (HSV1)
FT thymidine kinase (tk) gene"
FT misc_binding >600..<2200
FT /note="SIT unique KpnI"
FT misc_binding 3775..3775
FT /note="SIT double BamHI"
FT misc_binding 3962..3962
FT /note="SIT unique SphI"
FT misc_binding 4051..4051
FT /note="SIT unique SalI"
FT misc_binding 4825..4825
FT /note="SIT unique AvaI"
FT misc_binding 4844..4844
FT /note="SIT unique BalI"
FT misc_binding 5697..5697
FT /note="SIT unique NdeI"
FT rep_origin complement(5935..5935)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(5693..7556)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 7135..7135
FT /note="SIT unique PvuI"
XX
SQ Sequence 7940 BP; 1633 A; 2360 C; 2328 G; 1619 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc aagactgaca cattaaaaaa cacagaatcc gcggcacagc
ccgcggcccc gcgggcggcc aacccggcaa gcgcgcgcga gtgggccaaa aagcctagca
ggtcggagag gcagaccgcg ccgtttgcgt gggcggcgtt cacgaaagca aaacccgacg
tcgcgagcag ccccgttagg cgccagaaga gaggggggcg cgggccctgc tcggcgcccg
cgtcccccga gaaaaactcc gcgtatgccc gcgacaggaa ctgggcgtag ttcgtgccct
cctccgggta gccgcccacg cggcggaggg cgtccagcgc ggagccgttg tcggcccgcg
tcagggaccc taggacaaag acccgatacc gggggccgcc cgggggcccg ggaagagccc
ccggggggtt ttcgtccgcg gggtccccga cccgatctag cgtctggccc gcggggacca
ccatcacttc caccggaggg ctgtcgtgca tggatatcac gagccccatg aattcccgcc
cgtagcgcgc gcgcaccagc gcggcatcgc acccgagcac cagctccccc gtcgtccaga
tgcccacggg ccacgtcgag gccgacgggg agaaatacac gtacctacct ggggatctca
acaggccccg ggtggccaac caggtcgtgg acgcgttgtg caggtgcgtg atgtccagct
ccgtcgtcgg gtgccgccgg gccccaaccg gcggtcgggg gggcggtgta tcacgcggcc
cgctcgggtg gctcgccgtc gccacgttgt ctccccgcgg gaacgtcagg gcctcggggt
cagggacggc cgaaaacgtt acccaggccc gggaacgcag caacacggag gcggctggat
tgtgcaagag acccttaagg ggggcgaccg aggggggagg ctgggcggtc ggctcgaccg
tggtgggggc gggcaggctc gcgttcgggg gccggccgag caggtaggtc ttcgggatgt
aaagcagctg gccggggtcc cgcggaaact cggccgtggt gaccaataca aaacaaaagc
gctcctcgta ccagcgaaga aggggcagag atgccgtagt caggtttagt tcgtccggcg
gcgccagaaa tccgcgcggt ggtttttggg ggtcgggggt gtttggcagc cacagacgcc
cggtgttcgt gtcgcgccag tacatgcggt ccatgcccag gccatccaaa aaccatgggt
ctgtctgctc agtccagtcg tggacctgac cccacgcaac gcccaaaata ataaccccca
cgaaccataa accattcccc atgggggacc ccgtccctaa cccacggggc ccgtggctat
ggcagggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc caatggggtc tcggtggggt
atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
ccttccgtgt ttcagttagc ctcccccatc tcccgggcaa acgtgcgcgc caggtcgcag
atcgtcggta tggagcctgg ggtggtgacg tgggtctgga ccatcccgga ggtaagttgc
agcagggcgt cccggcagcc ggcgggcgat tggtcgtaat ccaggataaa gacatgcatg
ggacggaggc gtttggccaa gacgtccaaa gcccaggcaa acacgttata caggtcgccg
ttgggggcca gcaactcggg ggcccgaaac agggtaaata acgtgtcccc gatatggggt
cgtgggcccg cgttgctctg gggctcggca ccctggggcg gcacggccgc ccccgaaagc
tgtccccaat cctcccgcca cgacccgccg ccctgcagat accgcaccgt attggcaagc
agcccataaa cgcggcgaat cgcggccagc atagccaggt caagccgctc gccggggcgc
tggcgtttgg ccaggcggtc gatgtgtctg tcctccggaa gggcccccaa cacgatgttt
gtgccgggca aggtcggcgg gatgagggcc acgaacgcca gcacggcctg gggggtcatg
ctgcccataa ggtatcgcgc ggccgggtag cacaggaggg cggcgatggg atggcggtcg
aagatgaggg tgagggccgg gggcggggca tgtgagctcc cagcctcccc cccgatatga
ggagccagaa cggcgtcggt cacggcataa ggcatgccca ttgttatctg ggcgcttgtc
attaccaccg ccgcgtcccc ggccgatatc tcaccctggt cgaggcggtg ttgtgtggtg
tagatgttcg cgattgtctc ggaagccccc aacacccgcc agtaagtcat cggctcgggt
acgtagacga tatcgtcgcg cgaacccagg gccaccagca gttgcgtggt ggtggttttc
cccatcccgt ggggaccgtc tatataaacc cgcagtagcg tgggcatttt ctgctccagg
cggacttccg tggctttttg ttgccggcga gggcgcaacg ccgtacgtcg gttgttatgg
ccgcgagaac gcgcagcctg gtcgaacgca gacgcgtgtt gatggcaggg gtacgaagcc
atacgcgctt ctacaaggcg ctggccgaag aggtgcggga gtttcacgcc accaagatct
gcggcacgct gttgacgctg ttaagcgggt cgctgcaggg tcgctcggta ttcgaggcca
cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg ccgccccgac tgcatctgcg
tgttcgaatt cgccaatgac aagacgctgg gcggggtttg tgtcatcata gaactaaaga
catgcaaata tatttcttcc ggggacaccg ccagcaaacg cgagcaacgg gccacgggga
tgaagcagct gcgccactcc ctgaagctcc tgcagtccct cgcgcctccg ggtgacaaga
tagtgtacct gtgccccgtc ctggtgtttg tcgcccaacg gacgctccgc gtcagccgcg
tgacccggct cgtcccgcag aaggtctccg gtaatatcac cgcagtcgtg cggatgctcc
agagcctgtc cacgtatacg gtccccattg agcctaggac ccagcgagcc cgtcgccgcc
gcggcggcgc cgcccggggg tctgcgagca gaccgaaaag gtcacactct ggggcgcgcg
acccgcccga gtcagcggcc cgccagttac cacccgccga ccaaaccccc acctccacgg
agggcggggg ggtgcttaag aggatcgcgg cgctcttctg cgtgcccgtg gccaccaaga
ccaaaccccg agccgcctcc gaatgagagt gtttcgttcc ttccccctcc ccccgcgtca
gacaaaccct aaccaccgct taagcggccc ccgcgaggtc cgaagactca tttggatcct
ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc
agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta
tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg
atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
ggccctttcg tcttcaagaa
//