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ID   PHT3T7BMM  preliminary; circular DNA; SYN; 3006 BP.
XX
AC   IG0169;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHT3T7bm- - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pHT3T7 from pBR322 & T3 & T7
RC   pHT3T7bm+, pHT3T7bm- from pHT3T7
RA   ;
RT   ;
RL   Unpublished (1990).
XX
CC   NM (pHT3T7bm-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Boehringer)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pHT3T7)
CC   BR (pHT3T7bm+)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. T3, promoter
FT                   3. T7, promoter
FT                   -> pHT3T7
FT                   1. pHT3T7
FT                   2. oligo, MCS
FT                   -> pHT3T7bm- 3006bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3006 BP; 754 A; 757 C; 777 G; 718 T; 0 other;
     gggcgaattc ccggggatcc acgcgtaact taagtgcggc cgcggtaccg ggcccgtcga
     ctctagagct ctgcaggcat gcgatatccc atggaagctt ttgttccctt tagtgagggt
     taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctctg actggtgagt
     actcaaccaa gtcattctga gaatagtgta tgcggcgagc gagttgctct tgcccggcgt
     caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     cccgaaaagt gccacctgct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttccgaaatc
     ggcaaaatcc cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt
     tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg
     tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     ctacagggcg cgtatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     actata
//