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ID   PIB8       preliminary; circular DNA; SYN; 5406 BP.
XX
AC   U00003;
XX
DT   01-DEC-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli cosmid vector pIB8 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5406
RC   pIB8 from HomerI
RA   Lakey N., Ghazizadeh H., Gryan G., Jaehn L., Mintz L.,
RA   Richterich P., Robison K., Church G.M.;
RT   "Complete sequence of cosmid vector pIB";
RL   Unpublished (1993).
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RN   [2]
RP   1-5406
RC   pIB8
RA   Robison K.;
RT   ;
RL   Submitted (05-MAY-1993) by:
RL   Robison K., .
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CC   pIB8 was derived from pHomerI by insertion of a BamHI site at a
CC   completely filled-in EcoRI site. [2]
CC   pHomerI was derived from pAT153 [3] by insertion of the cos region
CC   of Charon 4 into a BstI site. [4]
CC   NM (pIB8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pHomerI from pAT153 and Charon 4)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. HomerI EcoRI
FT                   fill in
FT                   BamHI linker 6bp ggatcc
FT                   -> pIB8 5406bp"
FT   misc_binding    join(5406,1..5)
FT                   /note="SIT BamHI; cloning site"
FT   misc_binding    7..12
FT                   /note="SIT EcoRI; for liberating insert"
FT   misc_feature    10..5401
FT                   /note="from pHomerI;  pIB8 was constructed from
FT                   pHomer by filling in the unique EcoRI site and
FT                   ligating-in a BamHI linker"
FT   CDS             218..1078
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2038..2043
FT                   /note="SIT HaeII; marking deletion of
FT                   copy-number controlling region of pBR322"
FT   misc_feature    complement(join(2415..3306,5021..5336))
FT                   /note="remnant of tetA gene, destroyed during pHomerI
FT                   construction; also 2 frameshifts."
FT   misc_binding    3010..3015
FT                   /note="SIT SalI; used to generate left arm"
FT   misc_binding    4609..4620
FT                   /note="SIT phage lambda cohesive site; cos gene
FT                   lacking a coding region."
FT   misc_binding    5370..5375
FT                   /note="SIT HindIII; used to generate left arm"
FT   misc_binding    5399..5405
FT                   /note="SIT EcoRI; for liberating insert"
XX
SQ   Sequence 5406 BP; 1354 A; 1350 C; 1441 G; 1261 T; 0 other;
     gatccggaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     tttgtgatgc tcgtcagggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gctgacttcc gcgtttccag
     actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg
     ccaggaccca acgctgcccg agatgccgcg tgcggctgct ggagatggcg gacgcgatgg
     atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
     caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
     tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
     cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
     ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcggcat cccgatgccg
     ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     agtcccccgg ccacgggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcttg gcagtcacag
     catgcgcata tccatgcttc gaccatgcgc tcacaaagta ggtgaatgcg caatgtagta
     cccacatcgt catcgctttc cactgctctc gcgaataaag atggaaaatc aatctcatgg
     taatagtcca tgaaaatcct tgtattcata aatcctccag gtagctatat gcaaattgaa
     acaaaagaga tggtgatctt tctaagagat gatggaatct cccttcagta tcccgatggt
     caatgcgctg gatatgggat agatgggaat atgctgattt ttatgggaca gagttgcgaa
     ctgttcccaa ctaaaatcat tttgcacgat cagcgcacta cgaactttac ccacaaatag
     tcaggtaatg aatcctgata taaagacagg ttgataaatc agtcttctac gcgcatcgca
     cgcgcacacc gtagaaagtc tttcagttgt gagcctgggc aaaccgttaa ctttcggcgg
     ctttgctgtg cgacaggctc acgtctaaaa ggaaataaat catgggtcat aaaattatca
     cgttgtccgg cgcggcgacg gatgttctgt atgcgctgtt tttccgtggc gcgttgctgt
     ctggtgatct gccttctaaa tctggcacag ccgaattgcg cgagcttggt tttgctgaaa
     ccagacacac agcaactgaa taccagaaag aaaatcactt tacctttctg acatcagaag
     ggcagaaatt tgccgttgaa cacctggtca atacgcgttt tggtgagcag caatattgcg
     cttcgatgac gcttggcgtt gagattgata cctctgctgc acaaaaggca atcgacgagc
     tggaccagcg cattcgtgac accgtctcct tcgaacttat tcgcaatgga gtgtcattca
     tcaaggacgc cgctatcgca aatggtgcta tccacgcagc ggcaatcgaa acacctcagc
     cggtgaccaa tatctacaac atcagccttg gtatccagcg tgatgagcca gcgcagaaca
     aggtaaccgt cagtgccgat aagttcaaag ttaaacctgg tgttgatacc aacattgaaa
     cgttgatcga aaacgcgctg aaaaacgctg ctgaatgtgc ggcgctggat gtcacaaagc
     aaatggcagc agacaagaaa gcgatggatg aactggcttc ctatgtccgc acggccatca
     tgatggaatg tttccccggt ggtgttatct ggcagcagtg ccgtcgatag tatgcaattg
     ataattatta tcatttgcgg gtcctttccg gcgatccgcc ttgttacggg gcggcgacct
     cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc
     ataacttaat gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa
     agcgagcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg
     gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac
     tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat
     gactctgccg ccgtcataaa atggtatgcc gaaagggatg ctgaaattga gaacgaaaag
     ctgcgccggg aggttgaaga actgcggcag gccagcgagg cagatccaca ggacgggtgt
     ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg
     gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc
     atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg
     caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt
     gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca
     atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga
     attccg
//