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ID   PIBI24     preliminary; circular DNA; SYN; 2878 BP.
XX
AC   L08876; VB0073;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIBI24 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2878
RC   pIBI24
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Citation sequence information from IBI. Copyright by IBI
CC   NM (pIBI24)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (IBI)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL8+)(pUC18)(PromT7)(PF1)
CC   BR (pIBI25)
CC   OF (pIBI30)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp
FT                   2. T7 192bp, promoter
FT                   -> pIBI24 2878bp"
FT   misc_feature    2..235
FT                   /note="from pUC18"
FT   promoter        236..255
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    255..300
FT                   /note="from pUC18"
FT   misc_binding    255..300
FT                   /note="MCS EcoRI-SalI-(BamHI);
FT                   M13mp18/pUC18 polylinker"
FT   misc_feature    317..489
FT                   /note="from pUC18"
FT   misc_feature    491..946
FT                   /note="from bacteriophage f1"
FT   misc_feature    946..2878
FT                   /note="from pUC18"
FT   CDS             1147..1935
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2878 BP; 702 A; 721 C; 718 G; 737 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaatttaata
     cgactcacta tagggaattc gagctcggta cccggggatc ctctagagtc gacctgcagg
     ggccctcgag acgcgtggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac
     tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     cgtagtgggc catcgccccg atagacggtt tttcgccctt tgacgctgga gttcacgttc
     ctcaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     tttgatttat aagggatttt tccgatttcg gcctattggt taaaaaatga gctgatttaa
     caaaaattta acgcgaattt taacaaaata ttaacgttta taatttcagg tggcatcttt
     cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     gtgggttaca tcgaactgga tctcaatagt ggtaagatcc ttgagagttt tcgccccgaa
     gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     gtagtaatgg taacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     gccagcaacg cggccttttt acggttcctg gccttttgct gcggttttgc tcacatgttc
     tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//