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ID   PIBI25     preliminary; circular DNA; SYN; 2887 BP.
XX
AC   L08877; VB0074;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIBI25 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2887
RC   pIBI25
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Citation sequence information from IBI. copyright by IBI.
CC   NM (pIBI25)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (IBI)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pEMBL9+)(pUC19)(PromT7)(PF1)
CC   BR (pIBI24)
CC   OF (pIBI31)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. T7 201bp, promoter
FT                   -> pIBI24 2887bp"
FT   misc_feature    2..234
FT                   /note="from pUC19"
FT   promoter        245..264
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    296..498
FT                   /note="from pUC19"
FT   misc_binding    296..341
FT                   /note="MCS (BamHI)-SalI-EcoRI;
FT                   M13mp19/pUC19 polylinker"
FT   misc_feature    317..489
FT                   /note="from pUC19"
FT   misc_feature    500..955
FT                   /note="from bacteriophage f1"
FT   misc_feature    955..2887
FT                   /note="from pUC19"
FT   CDS             1156..1944
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2887 BP; 707 A; 726 C; 716 G; 738 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagatatc
     aaattaatac gactcactat agggagaagc ttgcatgcca cgcgtctcga gggcccctgc
     aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa
     cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     gatggttcac gtagtgggcc atcgccccga tagacggttt ttcgcccttt gacgctggag
     ttcacgttcc tcaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     gtctattctt ttgatttata agggattttt ccgatttcgg cctattggtt aaaaaatgag
     ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttat aatttcaggt
     ggcatctttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     ggtgcacgag tgggttacat cgaactggat ctcaatagtg gtaagatcct tgagagtttt
     cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     acgatgcctg tagtaatggt aacaacgttg cgcaaactat taactggcga actacttact
     ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg cggttttgct
     cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     gcggaag
//