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ID   PIBI30     preliminary; circular DNA; SYN; 2926 BP.
XX
AC   L08878; VB0075;
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DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIBI30 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2926
RC   pIBI30
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Citation sequence information from IBI. copyright by IBI.
CC   NM (pIBI30)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (IBI)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIBI24)
CC   BR (pIBI31)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIBI24 2878bp
FT                   2. T3 48bp, promoter
FT                   -> pIBI30 2926bp"
FT   misc_feature    2..231
FT                   /note="from pUC18"
FT   promoter        242..261
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    266..312
FT                   /note="from pUC18"
FT   misc_binding    267..312
FT                   /note="MCS EcoRI-SalI-(BamHI);
FT                   M13mp18/pUC18 polylinker"
FT   misc_feature    complement(345..364)
FT                   /note="PRO bacteriophage T3"
FT   misc_feature    373..537
FT                   /note="from pUC18"
FT   misc_feature    539..994
FT                   /note="from bacteriophage f1"
FT   misc_feature    994..2926
FT                   /note="from pUC18"
FT   CDS             1195..1983
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2926 BP; 713 A; 730 C; 730 G; 753 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac ggatatcgaa
     ttaatacgac tcactatagg gagatcgaat tcgagctcgg tacccgggga tcctctagag
     tcgacctgca ggggccctcg agacgcgtgg catgcaagct ttctcccttt agtgagggtt
     aattataggc ctagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     tcgccccgat agacggtttt tcgccctttg acgctggagt tcacgttcct caatagtgga
     ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     gggatttttc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     gcgaatttta acaaaatatt aacgtttata atttcaggtg gcatctttcg gggaaatgtg
     cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     gaactggatc tcaatagtgg taagatcctt gagagttttc gccccgaaga acgttttcca
     atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agtaatggta
     acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     gcctttttac ggttcctggc cttttgctgc ggttttgctc acatgttctt tcctgcgtta
     tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//