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ID   PIBI31     preliminary; circular DNA; SYN; 2923 BP.
XX
AC   L08879; VB0076;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pIBI31 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2923
RC   pIBI31
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Citation sequence information from IBI. copyright by IBI.
CC   NM (pIBI31)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (IBI)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIBI25)
CC   BR (pIBI30)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIBI25 2887bp
FT                   2. T3 36bp, promoter
FT                   -> pIBI31 2923bp"
FT   misc_feature    2..234
FT                   /note="from pUC19"
FT   promoter        245..264
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    296..341
FT                   /note="from pUC19"
FT   misc_binding    296..341
FT                   /note="MCS (BamHI)-SalI-EcoRI;
FT                   M13mp18/pUC18; polylinker"
FT   misc_feature    complement(345..364)
FT                   /note="PRO bacteriophage T3"
FT   misc_feature    373..534
FT                   /note="from pUC19"
FT   misc_feature    536..991
FT                   /note="from bacteriophage f1"
FT   misc_feature    991..2923
FT                   /note="from pUC19"
FT   CDS             1192..1980
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2923 BP; 715 A; 734 C; 723 G; 751 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagatatc
     aaattaatac gactcactat agggagaagc ttgcatgcca cgcgtctcga gggcccctgc
     aggtcgactc tagaggatcc ccgggtaccg agctcgaatt ctctcccttt agtgagggtt
     aattacaggc ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     ccccgataga cggtttttcg ccctttgacg ctggagttca cgttcctcaa tagtggactc
     ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     atttttccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     aattttaaca aaatattaac gtttataatt tcaggtggca tctttcgggg aaatgtgcgc
     ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     ctggatctca atagtggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagt aatggtaaca
     acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     tttttacggt tcctggcctt ttgctgcggt tttgctcaca tgttctttcc tgcgttatcc
     cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//