back Return to this vector's summary.
ID   PIC19H     preliminary; circular DNA; SYN; 2701 BP.
XX
AC   L08910; VB0091; K02759; K02756; ATCC37408;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pIC19H - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pIC7 from pUC8 & oligo
RC   M13mIC7 from M13mp10 & oligo
RC   pIC19R from pIC7 & pUC9
RC   pIC20R from pIC7 & pUC19
RC   pIC19H from pIC7 & pUC9
RC   pIC20H from pIC7 & pUC19
RC   pICEM19R+ from pIC7 & pEMBL8+
RC   pICEM19R- from pIC7 & pEMBL8-
RC   pICEM19H+ from pIC7 & pEMBL8+
RC   pICEM19H- from pIC7 & pEMBL8+
RA   Marsh J.L., Erfle M., Wykes E.J.;
RT   "The pIC plasmid and phage vectors with versatile cloning sites
RT   for recombinant selection by insertional inactivation";
RL   Gene 32:481-485(1984).
XX
RN   [2]
RP   1-2701
RC   pIC19H
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pUC9 and pIC7 by F. Pfeiffer
CC   For construction of pIC7, a synthetic oligonucleotide has
CC   been used to replace the pUC8 polylinker and thus to construct
CC   a new cloning vector with a different polylinker. The other
CC   pIC-vectors are based on this new pIC7 polylinker, which was
CC   combined with the existing pUC9 and pUC19 polylinkers in the
CC   following arrangements:
CC   pIC19 and pICEM19 vectors:
CC   pIC19R:   EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI
CC   pIC19H: HindIII- Poly (pUC9) -EcoRI  - Poly (pIC7) -HindIII
CC   pIC20-vectors:
CC   pIC20R:   EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI
CC   pIC20H: HindIII- Poly(pUC19) -EcoRI  - Poly (pIC7) -HindIII
CC
CC   To produce greater versatility of insertional inactivation of
CC   beta-galactosidase activity for subcloning and sequencing, a
CC   chemically synthesized oligonucleotide, specifying nine
CC   restriction sites including BglII, XhoI, NruI, ClaI, SacI and
CC   EcorV in various configurations with existing polylinkers, was
CC   created. These improved polylinkers were inserted into plasmids
CC   for routine cloning of ds-DNA and into chimeric phage/plasmids
CC   for biological production of ss-DNA.  The most versatile
CC   polyrecognition pattern specifies 17 restriction sites in the
CC   beta-galactosidase alpha-complementing gene fragment. Clone
CC   pIC7 was used to produce all the other polylinker-carrying
CC   vectors.
CC   A general purpose plasmid vector with a polylinker containing 13
CC   sites in lacZ'.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pIC19H)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(E.coli)(E.coli JM83)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIC7)(pUC9)
CC   BR (pIC19R)
CC   OF (pICEM19H-)(pICEM19H+)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIC7 small EcoRI-NarI 198bp 231..429
FT                   2. pUC9 NarI-EcoRI 2503bp 426..2665..264
FT                   -> pIC19H 2701bp"
FT   misc_binding    233..268
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI
FT                   polylinker of pUC9"
FT   misc_binding    263..301
FT                   /note="MCS ClaI-EcoRV-XbaI-BglII-XhoI-SacI-NruI-
FT                   HindIII; polylinker of pIC7"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             967..1755
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..269
FT                   /note="from pUC9"
FT   misc_feature    304..2701
FT                   /note="from pUC9"
XX
SQ   Sequence 2701 BP; 663 A; 688 C; 680 G; 670 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     ctgcaggtcg acggatcccc gggaattcat cgatatctag atctcgagct cgcgaaagct
     tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     a
//