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ID   PIC19R     preliminary; circular DNA; SYN; 2695 BP.
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AC   L08911; VB0092; K02760; K02757; ATCC37407;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIC19R - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pIC7 from pUC8 & oligo
RC   M13mIC7 from M13mp10 & oligo
RC   pIC19R from pIC7 & pUC9
RC   pIC20R from pIC7 & pUC19
RC   pIC19H from pIC7 & pUC9
RC   pIC20H from pIC7 & pUC19
RC   pICEM19R+ from pIC7 & pEMBL8+
RC   pICEM19R- from pIC7 & pEMBL8-
RC   pICEM19H+ from pIC7 & pEMBL8+
RC   pICEM19H- from pIC7 & pEMBL8+
RA   Marsh J.L., Erfle M., Wykes E.J.;
RT   "The pIC plasmid and phage vectors with versatile cloning sites
RT   for recombinant selection by insertional inactivation";
RL   Gene 32:481-485(1984).
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RN   [2]
RP   1-2695
RC   pIC19R
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pUC9 and pIC7 by F. Pfeiffer
CC   For construction of pIC7, a synthetic oligonucleotide has
CC   been used to replace the pUC8 polylinker and thus to construct
CC   a new cloning vector with a different polylinker. The other
CC   pIC-vectors are based on this new pIC7 polylinker, which was
CC   combined with the existing pUC9 and pUC19 polylinkers in the
CC   following arrangements:
CC   pIC19 and pICEM19 vectors:
CC   pIC19R:   EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI
CC   pIC19H: HindIII- Poly (pUC9) -EcoRI  - Poly (pIC7) -HindIII
CC   pIC20-vectors:
CC   pIC20R:   EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI
CC   pIC20H: HindIII- Poly(pUC19) -EcoRI  - Poly (pIC7) -HindIII
CC
CC   To produce greater versatility of insertional inactivation of
CC   beta-galactosidase activity for subcloning and sequencing, a
CC   chemically synthesized oligonucleotide, specifying nine
CC   restriction sites including BglII, XhoI, NruI, ClaI, SacI and
CC   EcorV in various configurations with existing polylinkers, was
CC   created. These improved polylinkers were inserted into plasmids
CC   for routine cloning of ds-DNA and into chimeric phage/plasmids
CC   for biological production of ss-DNA.  The most versatile
CC   polyrecognition pattern specifies 17 restriction sites in the
CC   beta-galactosidase alpha-complementing gene fragment. Clone
CC   pIC7 was used to produce all the other polylinker-carrying
CC   vectors.
CC   A general purpose plasmid vector with polylinker containing 13
CC   sites in lacZ'.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pIC19R)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIC7)(pUC9)
CC   BR (pIC19H)
CC   OF (pICEM19R-)(pICEM19R+)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIC7 HindIII-NarI 2503bp 429..2668..264,
FT                   \ ori/amp/MCS
FT                   2. pUC9 HindIII-NarI 192bp 234..426
FT                   -> pIC19R 2695bp"
FT   misc_binding    230..268
FT                   /note="MCS EcoRI-ClaI-EcoRV-XbaI-BglII-XhoI-SacI-NruI-
FT                   HindIII; polylinker of pIC7"
FT   misc_binding    263..298
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI
FT                   polylinker of pUC9"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             961..1749
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..230
FT                   /note="from pUC9"
FT   misc_feature    263..2695
FT                   /note="from pUC9"
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SQ   Sequence 2695 BP; 663 A; 685 C; 677 G; 670 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcatcga
     tatctagatc tcgagctcgc gaaagcttgg ctgcaggtcg acggatcccc gggaattcac
     tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//