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ID   PIC20H     preliminary; circular DNA; SYN; 2722 BP.
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AC   L08912; VB0093; K02756; ATCC37382;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIC20H - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pIC7 from pUC8 & oligo
RC   M13mIC7 from M13mp10 & oligo
RC   pIC19R from pIC7 & pUC9
RC   pIC20R from pIC7 & pUC19
RC   pIC19H from pIC7 & pUC9
RC   pIC20H from pIC7 & pUC19
RC   pICEM19R+ from pIC7 & pEMBL8+
RC   pICEM19R- from pIC7 & pEMBL8-
RC   pICEM19H+ from pIC7 & pEMBL8+
RC   pICEM19H- from pIC7 & pEMBL8+
RA   Marsh J.L., Erfle M., Wykes E.J.;
RT   "The pIC plasmid and phage vectors with versatile cloning sites
RT   for recombinant selection by insertional inactivation";
RL   Gene 32:481-485(1984).
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RN   [2]
RP   1-2722
RC   pIC20h
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pUC19 and pIC7 by F. Pfeiffer
CC   For construction of pIC7, a synthetic oligonucleotide has
CC   been used to replace the pUC8 polylinker and thus to construct
CC   a new cloning vector with a different polylinker. The other
CC   pIC-vectors are based on this new pIC7 polylinker, which was
CC   combined with the existing pUC9 and pUC19 polylinkers in the
CC   following arrangements:
CC   pIC19 and pICEM19 vectors:
CC   pIC19R:   EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI
CC   pIC19H: HindIII- Poly (pUC9) -EcoRI  - Poly (pIC7) -HindIII
CC   pIC20-vectors:
CC   pIC20R:   EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI
CC   pIC20H: HindIII- Poly(pUC19) -EcoRI  - Poly (pIC7) -HindIII
CC
CC   To produce greater versatility of insertional inactivation of
CC   beta-galactosidase activity for subcloning and sequencing, a
CC   chemically synthesized oligonucleotide, specifying nine
CC   restriction sites including BglII, XhoI, NruI, ClaI, SacI and
CC   EcorV in various configurations with existing polylinkers, was
CC   created. These improved polylinkers were inserted into plasmids
CC   for routine cloning of ds-DNA and into chimeric phage/plasmids
CC   for biological production of ss-DNA.  The most versatile
CC   polyrecognition pattern specifies 17 restriction sites in the
CC   beta-galactosidase alpha-complementing gene fragment. Clone
CC   pIC7 was used to produce all the other polylinker-carrying
CC   vectors.
CC   A general purpose plasmid vector with a polylinker containing 17
CC   sites in lacZ'.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pIC20H)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIC7)(pUC19)(pICEM20R)
CC   BR (pIC20H)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIC7 EcoRI-NarI 198bp 231..429
FT                   2. pUC19 NarI-EcoRI 2526bp 397..2686..237
FT                   -> pIC20H 2722bp"
FT   misc_binding    233..289
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-
FT                   KpnI-SacI-EcoRI
FT                   polylinker of pUC19"
FT   misc_binding    284..322
FT                   /note="MCS EcoRI-ClaI-EcoRV-XbaI-BglII-XhoI-SacI-
FT                   NruI-HindIII; polylinker of pIC7"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             988..1776
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..290
FT                   /note="from pUC19"
FT   misc_feature    325..2722
FT                   /note="from pUC19"
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SQ   Sequence 2722 BP; 668 A; 695 C; 684 G; 675 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
     atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca tcgatatcta
     gatctcgagc tcgcgaaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     cagtgagcga ggaagcggaa ga
//