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ID   PIC20R     preliminary; circular DNA; SYN; 2716 BP.
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AC   L08913; VB0094; K02757; ATCC37381;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIC20R - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pIC7 from pUC8 & oligo
RC   M13mIC7 from M13mp10 & oligo
RC   pIC19R from pIC7 & pUC9
RC   pIC20R from pIC7 & pUC19
RC   pIC19H from pIC7 & pUC9
RC   pIC20H from pIC7 & pUC19
RC   pICEM19R+ from pIC7 & pEMBL8+
RC   pICEM19R- from pIC7 & pEMBL8-
RC   pICEM19H+ from pIC7 & pEMBL8+
RC   pICEM19H- from pIC7 & pEMBL8+
RA   Marsh J.L., Erfle M., Wykes E.J.;
RT   "The pIC plasmid and phage vectors with versatile cloning sites
RT   for recombinant selection by insertional inactivation";
RL   Gene 32:481-485(1984).
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RN   [2]
RP   1-2716
RC   pIC20r
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pUC19 and pIC7 by F. Pfeiffer
CC   For construction of pIC7, a synthetic oligonucleotide has
CC   been used to replace the pUC8 polylinker and thus to construct
CC   a new cloning vector with a different polylinker. The other
CC   pIC-vectors are based on this new pIC7 polylinker, which was
CC   combined with the existing pUC9 and pUC19 polylinkers in the
CC   following arrangements:
CC   pIC19 and pICEM19 vectors:
CC   pIC19R:   EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI
CC   pIC19H: HindIII- Poly (pUC9) -EcoRI  - Poly (pIC7) -HindIII
CC   pIC20-vectors:
CC   pIC20R:   EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI
CC   pIC20H: HindIII- Poly(pUC19) -EcoRI  - Poly (pIC7) -HindIII
CC
CC   To produce greater versatility of insertional inactivation of
CC   beta-galactosidase activity for subcloning and sequencing, a
CC   chemically synthesized oligonucleotide, specifying nine
CC   restriction sites including BglII, XhoI, NruI, ClaI, SacI and
CC   EcorV in various configurations with existing polylinkers, was
CC   created. These improved polylinkers were inserted into plasmids
CC   for routine cloning of ds-DNA and into chimeric phage/plasmids
CC   for biological production of ss-DNA.  The most versatile
CC   polyrecognition pattern specifies 17 restriction sites in the
CC   beta-galactosidase alpha-complementing gene fragment. Clone
CC   pIC7 was used to produce all the other polylinker-carrying
CC   vectors.
CC   Streak to xgal + amp plate and pick a blue colony.
CC   A general purpose plasmid vector with a polylinker containing 17
CC   sites in lacZ'.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pIC20R)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIC7)(pUC19)(pICEM20H)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIC7 NarI-HindIII 2503bp 429..2668..264,
FT                   \ ori/amp/MCS
FT                   2. pUC19 NarI-HindIII 211bp 237..448
FT                   -> pIC20R 2716bp"
FT   misc_binding    230..268
FT                   /note="MCS EcoRI-ClaI-EcoRV-XbaI-BglII-XhoI-SacI-
FT                   NruI-HindIII; polylinker of pIC7"
FT   misc_binding    263..319
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-
FT                   KpnI-SacI-EcoRI
FT                   polylinker of pUC19"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             982..1770
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..230
FT                   /note="from pUC19"
FT   misc_feature    263..2716
FT                   /note="from pUC19"
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SQ   Sequence 2716 BP; 668 A; 692 C; 681 G; 675 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcatcga
     tatctagatc tcgagctcgc gaaagcttgc atgcctgcag gtcgactcta gaggatcccc
     gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     gcgaggaagc ggaaga
//