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ID   PICEM19HM  preliminary; circular DNA; SYN; 3973 BP.
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AC   L08914; K02759; VB0095; ATCC37378;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pICEM19H- - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pIC7 from pUC8 & oligo
RC   M13mIC7 from M13mp10 & oligo
RC   pIC19R from pIC7 & pUC9
RC   pIC20R from pIC7 & pUC19
RC   pIC19H from pIC7 & pUC9
RC   pIC20H from pIC7 & pUC19
RC   pICEM19R+ from pIC7 & pEMBL8+
RC   pICEM19R- from pIC7 & pEMBL8-
RC   pICEM19H+ from pIC7 & pEMBL8+
RC   pICEM19H- from pIC7 & pEMBL8+
RA   Marsh J.L., Erfle M., Wykes E.J.;
RT   "The pIC plasmid and phage vectors with versatile cloning sites
RT   for recombinant selection by insertional inactivation";
RL   Gene 32:481-485(1984).
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RN   [2]
RP   1-3973
RC   pICEM19H-
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pIC19H and pEMBL9m by F. Pfeiffer.
CC   For construction of pIC7, a synthetic oligonucleotide has
CC   been used to replace the pUC8 polylinker and thus to construct
CC   a new cloning vector with a different polylinker. The other
CC   pIC-vectors are based on this new pIC7 polylinker, which was
CC   combined with the existing pUC9 and pUC19 polylinkers in the
CC   following arrangements:
CC   pIC19 and pICEM19 vectors:
CC   pIC19R:   EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI
CC   pIC19H: HindIII- Poly (pUC9) -EcoRI  - Poly (pIC7) -HindIII
CC   pIC20-vectors:
CC   pIC20R:   EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI
CC   pIC20H: HindIII- Poly(pUC19) -EcoRI  - Poly (pIC7) -HindIII
CC   The pICEM minus phasmid vectors were prepared by exchanging a
CC   pEMBL8m segment containing the f1 origin against the
CC   pUC-derived fragment from the corresponding pIC-vector.
CC   The fragment of pEMBL8m used is identical to the corresponding
CC   fragment from pEMBL9m. Because pICEM19Hm contains the pUC9
CC   polylinker, pEMBL9m is given in the PARENT section although
CC   pEMBL8m has been used in the experiment.
CC   Streak to xgal + amp plate and pick a blue colony.
CC   Phagemid with polylinker in lacZ' for production of ssDNA.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pICEM19H-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIC19H)(pEMBL8-)(pIC7)(pUC9)(pEMBL9-)
CC   BR (pICEM19H+)(pICEM19R-)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIC19H BglI-BglI 1583bp 1570..2701..452,
FT                   \ lacZ gene [1581bp]
FT                   2. pEMBL8- BglI-BglI 2392bp 414..2806, f1 ori
FT                   -> pICEM19H- 3973bp"
FT   misc_binding    231..266
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI
FT                   polylinker of pUC9"
FT   misc_binding    261..299
FT                   /note="MCS ClaI-EcoRV-XbaI-BglII-XhoI-SacI-NruI-
FT                   HindIII; polylinker of pIC7"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             2241..3029
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..267
FT                   /note="from pEMBL9m"
FT   misc_feature    302..3975
FT                   /note="from pEMBL9m"
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SQ   Sequence 3973 BP; 1087 A; 947 C; 968 G; 971 T; 0 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttggct
     gcaggtcgac ggatccccgg gaattcatcg atatctagat ctcgagctcg cgaaagcttg
     gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     cgcccttccc aacagttgcg cagcctgaat ggcgaatgga attccaacat ccaataaatc
     atacaggcaa ggcaaagaat tagcaaaatt aagcaataaa gcctcagagc ataaagctaa
     atcggttgta ccaaaaacat tatgaccctg taatactttt gcgggagaag cctttatttc
     aacgcaagga taaaaatttt tagaaccctc atatatttta aatgcaatgc ctgagtaatg
     tgtaggtaaa gattcaaacg ggtgagaaag gccggagaca gtcaaatcac catcaatatg
     atattcaacc gttctagctg ataaattcat gccggagagg gtagctattt ttgagaggtc
     tctacaaagg ctatcaggtc attgcctgag agtctggagc aaacaagaga atcgatgaac
     ggtaatcgta aaactagcat gtcaatcata tgtaccccgg ttgataatca gaaaagcccc
     aaaaacagga agattgtata agcaaatatt taaattgtaa acgttaatat tttgttaaaa
     ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     ggcgcgtact atggttgctt tgacgagcac gtataacgtg ctttcctcgt tagaatcaga
     gcgggagcta aacaggaggc cgattaaagg gattttagac aggaacggta cgccagaatc
     ctgagaagtg tttttataat cagtgaggcc accgagtaaa agagtctgtc catcacgcaa
     attaaccgtt gtcgcaatac ttctttgatt agtaataaca tcacttgcct gagtagaaga
     actcaaacta tcggccttgc tggtaatatc cagaacaata ttaccgccag ccattgcaac
     aggaaaaacg ctcatggaaa tacctacatt ttgacgctca atcgtctgga attcctgatg
     cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     gaagcggaag agc
//