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ID PICEM19HP preliminary; circular DNA; SYN; 3973 BP.
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AC L08915; K02759; VB0096; ATCC37377;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pICEM19H+ - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pIC7 from pUC8 & oligo
RC M13mIC7 from M13mp10 & oligo
RC pIC19R from pIC7 & pUC9
RC pIC20R from pIC7 & pUC19
RC pIC19H from pIC7 & pUC9
RC pIC20H from pIC7 & pUC19
RC pICEM19R+ from pIC7 & pEMBL8+
RC pICEM19R- from pIC7 & pEMBL8-
RC pICEM19H+ from pIC7 & pEMBL8+
RC pICEM19H- from pIC7 & pEMBL8+
RA Marsh J.L., Erfle M., Wykes E.J.;
RT "The pIC plasmid and phage vectors with versatile cloning sites
RT for recombinant selection by insertional inactivation";
RL Gene 32:481-485(1984).
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RN [2]
RP 1-3973
RC pICEM19H+
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
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CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Assembled from pIC19H and pEMBL9m by F. Pfeiffer.
CC For construction of pIC7, a synthetic oligonucleotide has
CC been used to replace the pUC8 polylinker and thus to construct
CC a new cloning vector with a different polylinker. The other
CC pIC-vectors are based on this new pIC7 polylinker, which was
CC combined with the existing pUC9 and pUC19 polylinkers in the
CC following arrangements:
CC pIC19 and pICEM19 vectors:
CC pIC19R: EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI
CC pIC19H: HindIII- Poly (pUC9) -EcoRI - Poly (pIC7) -HindIII
CC pIC20-vectors:
CC pIC20R: EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI
CC pIC20H: HindIII- Poly(pUC19) -EcoRI - Poly (pIC7) -HindIII
CC The pICEM plus phasmid vectors were prepared by exchanging a
CC pEMBL8p segment containing the f1 origin against the
CC pUC-derived fragment from the corresponding pIC-vector.
CC The fragment of pEMBL8p used is identical to the corresponding
CC fragment from pEMBL9p. Because pICEM19Hp contains the pUC9
CC polylinker, pEMBL9p is given in the PARENT section although
CC pEMBL8p has been used in the experiment.
CC Chimeric phage/plasmid for cloning into lacZ' polylinker for
CC production of ssDNA.
CC Medium is 1227 LB plus ampicillin.
CC NM (pICEM19H+)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (ATCC)
CC HO (E.coli TB1)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pIC19H)(pEMBL8+)(pIC7)(pUC9)(pEMBL9+)
CC BR (pICEM19H-)(pICEM19R+)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pIC19H BglI-BglI 1583bp 1570..2701..452,
FT \ lacZ gene [1581bp]
FT 2. pEMBL8+ BglI-BglI 2392bp 414..2806, f1 ori
FT -> pICEM19H+ 3973bp"
FT misc_binding 231..266
FT /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI
FT polylinker of pUC9"
FT misc_binding 261..299
FT /note="MCS ClaI-EcoRV-XbaI-BglII-XhoI-SacI-NruI-
FT HindIII; polylinker of pIC7"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1"
FT CDS 2241..3029
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT misc_feature 1..267
FT /note="from pEMBL9p"
FT misc_feature 302..3975
FT /note="from pEMBL9p"
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SQ Sequence 3973 BP; 964 A; 976 C; 939 G; 1094 T; 0 other;
gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttggct
gcaggtcgac ggatccccgg gaattcatcg atatctagat ctcgagctcg cgaaagcttg
gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
cgcccttccc aacagttgcg cagcctgaat ggcgaatgga attccagacg attgagcgtc
aaaatgtagg tatttccatg agcgtttttc ctgttgcaat ggctggcggt aatattgttc
tggatattac cagcaaggcc gatagtttga gttcttctac tcaggcaagt gatgttatta
ctaatcaaag aagtattgcg acaacggtta atttgcgtga tggacagact cttttactcg
gtggcctcac tgattataaa aacacttctc aggattctgg cgtaccgttc ctgtctaaaa
tccctttaat cggcctcctg tttagctccc gctctgattc taacgaggaa agcacgttat
acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
taaatatttg cttatacaat cttcctgttt ttggggcttt tctgattatc aaccggggta
catatgattg acatgctagt tttacgatta ccgttcatcg attctcttgt ttgctccaga
ctctcaggca atgacctgat agcctttgta gagacctctc aaaaatagct accctctccg
gcatgaattt atcagctaga acggttgaat atcatattga tggtgatttg actgtctccg
gcctttctca cccgtttgaa tctttaccta cacattactc aggcattgca tttaaaatat
atgagggttc taaaaatttt tatccttgcg ttgaaataaa ggcttctccc gcaaaagtat
tacagggtca taatgttttt ggtacaaccg atttagcttt atgctctgag gctttattgc
ttaattttgc taattctttg ccttgcctgt atgatttatt ggatgttgga attcctgatg
cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
gaagcggaag agc
//