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ID   PICEM19RM  preliminary; circular DNA; SYN; 3967 BP.
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AC   L08916; K02760; VB0097; ATCC37380;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pICEM19R- - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pIC7 from pUC8 & oligo
RC   M13mIC7 from M13mp10 & oligo
RC   pIC19R from pIC7 & pUC9
RC   pIC20R from pIC7 & pUC19
RC   pIC19H from pIC7 & pUC9
RC   pIC20H from pIC7 & pUC19
RC   pICEM19R+ from pIC7 & pEMBL8+
RC   pICEM19R- from pIC7 & pEMBL8-
RC   pICEM19H+ from pIC7 & pEMBL8+
RC   pICEM19H- from pIC7 & pEMBL8+
RA   Marsh J.L., Erfle M., Wykes E.J.;
RT   "The pIC plasmid and phage vectors with versatile cloning sites
RT   for recombinant selection by insertional inactivation";
RL   Gene 32:481-485(1984).
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RN   [2]
RP   1-3973
RC   pICEM19R-
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pIC19H and pEMBL9m by F. Pfeiffer.
CC   For construction of pIC7, a synthetic oligonucleotide has
CC   been used to replace the pUC8 polylinker and thus to construct
CC   a new cloning vector with a different polylinker. The other
CC   pIC-vectors are based on this new pIC7 polylinker, which was
CC   combined with the existing pUC9 and pUC19 polylinkers in the
CC   following arrangements:
CC   pIC19 and pICEM19 vectors:
CC   pIC19R:   EcoRI- Poly (pIC7) -HindIII- Poly (pUC9) -EcoRI
CC   pIC19H: HindIII- Poly (pUC9) -EcoRI  - Poly (pIC7) -HindIII
CC   pIC20-vectors:
CC   pIC20R:   EcoRI- Poly (pIC7) -HindIII- Poly(pUC19) -EcoRI
CC   pIC20H: HindIII- Poly(pUC19) -EcoRI  - Poly (pIC7) -HindIII
CC   The pICEM minus phasmid vectors were prepared by exchanging a
CC   pEMBL8m segment containing the f1 origin against the
CC   pUC-derived fragment from the corresponding pIC-vector.
CC   The fragment of pEMBL8m used is identical to the corresponding
CC   fragment from pEMBL9m. Because pICEM19Rm contains the pUC9
CC   polylinker, pEMBL9m is given in the PARENT section although
CC   pEMBL8m has been used in the experiment.
CC   Chimeric phage/plasmid for cloning into lacZ' polylinker for
CC   production of ssDNA.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pICEM19R-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli TB1)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIC19R)(pEMBL8-)(pIC7)(pUC9)(pEMBL9-)
CC   BR (pICEM19H-)(pICEM19R+)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIC19R BglI-BglI 1577bp 1564..2695..446,
FT                   \ lacZ gene [1575bp]
FT                   2. pEMBL8- BglI-BglI 2392bp 414..2806, f1 ori
FT                   -> pICEM19R- 3967bp"
FT   misc_binding    228..266
FT                   /note="MCS EcoRI-ClaI-EcoRV-XbaI-BglII-XhoI-SacI-NruI-
FT                   HindIII; polylinker of pIC7"
FT   misc_binding    261..296
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI
FT                   polylinker of pUC9"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             2235..3023
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..228
FT                   /note="from pEMBL9m"
FT   misc_feature    361..3969
FT                   /note="from pEMBL9m"
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SQ   Sequence 3967 BP; 1087 A; 944 C; 965 G; 971 T; 0 other;
     gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcatcgata
     tctagatctc gagctcgcga aagcttggct gcaggtcgac ggatccccgg gaattcactg
     gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     tcccaacagt tgcgcagcct gaatggcgaa tggaattcca acatccaata aatcatacag
     gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag ctaaatcggt
     tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta tttcaacgca
     aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt aatgtgtagg
     taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa tatgatattc
     aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga ggtctctaca
     aaggctatca ggtcattgcc tgagagtctg gagcaaacaa gagaatcgat gaacggtaat
     cgtaaaacta gcatgtcaat catatgtacc ccggttgata atcagaaaag ccccaaaaac
     aggaagattg tataagcaaa tatttaaatt gtaaacgtta atattttgtt aaaattcgcg
     ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     tactatggtt gctttgacga gcacgtataa cgtgctttcc tcgttagaat cagagcggga
     gctaaacagg aggccgatta aagggatttt agacaggaac ggtacgccag aatcctgaga
     agtgttttta taatcagtga ggccaccgag taaaagagtc tgtccatcac gcaaattaac
     cgttgtcgca atacttcttt gattagtaat aacatcactt gcctgagtag aagaactcaa
     actatcggcc ttgctggtaa tatccagaac aatattaccg ccagccattg caacaggaaa
     aacgctcatg gaaataccta cattttgacg ctcaatcgtc tggaattcct gatgcggtat
     tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     gaagagc
//