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ID   PICT19H    preliminary; circular DNA; SYN; 3042 BP.
XX
AC   X53126;
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DT   15-AUG-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   C. elegans plasmid vector pICT19h - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3042
RC   pICT19h
RA   Fire A.Z.;
RT   ;
RL   Submitted (17-MAY-1990) on tape to the EMBL Data Library by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
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RN   [2]
RP   1-3042
RC   pAST18a from pAST & pUC18
RC   pAST18b from pAST & pUC18
RC   pAST19a from pAST & pUC19
RC   pAST19b from pAST & pUC19
RC   pICT19h from pAST & pIC19H
RC   pICT19r from pAST & pIC19R
RC   pPD26.14 from pAST19b & linker
RA   Fire A.Z., Kondo K., Waterson R.;
RT   "Vectors for low copy transformation of C. elegans";
RL   Nucleic Acids Res. 18:4269-4270(1990).
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CC   pICT19h combines plasmid pICEM19h or PIC19h (K02759),
CC   C. elegans sup-7 sequence (K02459),
CC   and pUC19 sequence (M11662).
CC   NM (pICT19h)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (C.elegans)
CC   CP (low)
CC   FN (cloning)
CC   SE ()
CC   PA (pIC19H)(pICEM19H)(C.elegans sup-7)(pUC19)
CC   BR (pICT19r)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. C.elegans sup-7 locus
FT                   -> pRW91
FT                   1. pRW91 EcoRI-SalI 1300bp, C.elegans sup-7
FT                   2. pUC12 remove EcoRI-SalI 30bp 231..261, MCS/2650bp
FT                   -> pAst 4000bp
FT                   1. pIC19H SalI-XbaI 2672bp 277..2701..248, MCS
FT                   2. pAst SalI-XbaI 1300bp, sup-7/pUC12 255..-..261
FT                   -> pICT19h 3042bp"
FT   misc_feature    1..425
FT                   /note="from pICEM19h"
FT   misc_feature    426..794
FT                   /note="from C. elegans sup-7 locus"
FT   misc_feature    795..3042
FT                   /note="from pIC19h"
XX
SQ   Sequence 3042 BP; 781 A; 734 C; 739 G; 788 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcgcga gctcgagatc
     tagatacaat tttcagaata cgttttttgt gggcttgggt atattgtttt taatgttata
     cttgcagtcg tgaaatttga ttttcaaatt tgtagaaaaa tcaagaaaat aattgcaaca
     ttcgcttgtg tcaaaaacca atttcaacaa attttcgtgt gagaaataca ttaccagaag
     gcattttttc acacgattag cattttggac tactttatta aatttttgcg tgtaattttg
     aattaaattg tattatatta ctacttaaaa aacaaaaaat ttgaccactg agcggatcga
     acgcccaacc tttcgatcta gagtcgaacg cgctaccatt gcgccaagca gtcatgttat
     tctctcttgt cgacctgcag ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//