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ID   PICT19R    preliminary; circular DNA; SYN; 3023 BP.
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AC   X53125;
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DT   15-AUG-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   C. elegans plasmid vector pICT19r - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3023
RC   pICT19r
RA   Fire A.Z.;
RT   ;
RL   Submitted (17-MAY-1990) on tape to the EMBL Data Library by:
RL   Fire A.Z., Carnegie Inst. of Washington, Dept. Embryology,
RL   115 West University Parkway, Baltimore MD 21210, USA.
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RN   [2]
RP   1-3023
RC   pAST18a from pAST & pUC18
RC   pAST18b from pAST & pUC18
RC   pAST19a from pAST & pUC19
RC   pAST19b from pAST & pUC19
RC   pICT19h from pAST & pIC19H
RC   pICT19r from pAST & pIC19R
RC   pPD26.14 from pAST19b & linker
RA   Fire A.Z., Kondo K., Waterson R.;
RT   "Vectors for low copy transformation of C. elegans";
RL   Nucleic Acids Res. 18:4269-4270(1990).
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CC   pICT19r combines plasmid pICEM19r or PIC19r (K02760),
CC   C. elegans sup-7 sequence (K02459),
CC   and pUC19 sequence (M11662).
CC   NM (pICT19r)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (C.elegans)
CC   CP (low)
CC   FN (cloning)
CC   SE ()
CC   PA (pIC19R)(pICEM19R)(C.elegans sup-7)(pUC19)
CC   BR (pICT19h)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. C.elegans sup-7 locus
FT                   -> pRW91
FT                   1. pRW91 EcoRI-SalI 1300bp, C.elegans sup-7
FT                   2. pUC12 remove EcoRI-SalI 30bp 231..261, MCS/2650bp
FT                   -> pAst 4000bp
FT                   1. pIC19R XbaI-SalI 2661bp 278..2695..244, MCS
FT                   2. pAst SalI-XbaI 1300bp, sup-7/pUC12 255..-..261
FT                   -> pICT19r 3023bp"
FT   misc_feature    1..411
FT                   /note="from pIC19r"
FT   misc_feature    412..780
FT                   /note="from C. elegans sup-7 locus"
FT   misc_feature    781..3023
FT                   /note="from pIC19r"
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SQ   Sequence 3023 BP; 792 A; 724 C; 736 G; 771 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cccggggatc cgtcgacaag
     agagaataac atgactgctt ggcgcaatgg tagcgcgttc gactctagat cgaaaggttg
     ggcgttcgat ccgctcagtg gtcaaatttt ttgtttttta agtagtaata taatacaatt
     taattcaaaa ttacacgcaa aaatttaata aagtagtcca aaatgctaat cgtgtgaaaa
     aatgccttct ggtaatgtat ttctcacacg aaaatttgtt gaaattggtt tttgacacaa
     gcgaatgttg caattatttt cttgattttt ctacaaattt gaaaatcaaa tttcacgact
     gcaagtataa cattaaaaac aatataccca agcccacaaa aaacgtattc tgaaaattgt
     atcgatgaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     gcgtatcacg aggccctttc gtc
//