back Return to this vector's summary.
ID   PIJ2921    preliminary; circular DNA; SYN; 2710 BP.
XX
AC   X69144;
XX
DT   15-DEC-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pIJ2921 - complete, lacZ-alpha.
XX
KW   cloning vector; alpha-complementation; modified lacZ-alpha.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-95
RC   pIJ2921
RA   Bibb M.J.;
RT   ;
RL   Submitted (09-NOV-1992) to the EMBL/GenBank/DDBJ databases by:
RL   Bibb M.J., John Innes Institute, John Innes Centre, Colney Lane,
RL   Norwich, NR4 7UH, UK.
XX
RN   [2]
RP   1-95
RC   pIJ2920 from pUC18 & linker
RC   pIJ2921, pIJ2922 from pIJ2920 & linker
RC   pIJ2925, pIJ2926 from pIJ2922 & pIJ486
RA   Janssen G.R., Bibb M.J.;
RT   "Derivatives of pUC18 that have BglII sites flanking a modified
RT   multiple cloning site and that retain the ability to identify
RT   recombinant clones by visual screening of Escherichia coli";
RL   Gene 124:133-134(1993).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NCBI gi: 58011
CC   NM (pIJ2921)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC18)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIJ2920 EcoRI 2700bp, pUC18 451..451
FT                   Klenow
FT                   2. BglII linker 8bp cagatctg
FT                   -> pIJ2921 2700bp"
FT   -               1..403
FT                   /note="pUC18 1..403 403bp
FT                   HindIII = A^AGCT T
FT                   \                cagatctg"
FT   -               404..411
FT                   /note="cagatctg 8bp
FT                   \  cagatctg
FT                   HindIII = A^AGCTT"
FT   -               412..466
FT                   /note="pUC18 400..454 55bp
FT                   EcoRI =   G^AATT C
FT                   \                cagatctg"
FT   -               467..474
FT                   /note="cagatctg 8bp
FT                   \  cagatctg
FT                   EcoRI =   G^AATTC"
FT   -               475..2710
FT                   /note="pUC18 451..2686 2236bp"
FT   CDS             6..>95
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   modified alpha peptide; NCBI gi: 58012"
XX
SQ   Sequence 2710 BP; 675 A; 682 C; 688 G; 665 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctcagatct gagcttgcat
     gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcaga tctgaattcg
     taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     tctcaaagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     ccctttcgtc
//