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ID   PIJ2922    preliminary; circular DNA; SYN; 2702 BP.
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AC   X69146;
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DT   15-DEC-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIJ2922 - complete, lacZ-alpha.
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KW   cloning vector; alpha-complementation; modified lacZ-alpha.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-83
RC   pIJ2922
RA   Bibb M.J.;
RT   ;
RL   Submitted (09-NOV-1992) to the EMBL/GenBank/DDBJ databases by:
RL   Bibb M.J., John Innes Institute, John Innes Centre, Colney Lane,
RL   Norwich, NR4 7UH, UNITED KINGDOM.
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RN   [2]
RP   1-83
RC   pIJ2920 from pUC18 & linker
RC   pIJ2921, pIJ2922 from pIJ2920 & linker
RC   pIJ2925, pIJ2926 from pIJ2922 & pIJ486
RA   Janssen G.R., Bibb M.J.;
RT   "Derivatives of pUC18 that have BglII sites flanking a modified
RT   multiple cloning site and that retain the ability to identify
RT   recombinant clones by visual screening of Escherichia coli";
RL   Gene 124:133-134(1993).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NCBI gi: 58015
CC   NM (pIJ2922)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC18)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pIJ2920 EcoRI 2700bp
FT                   S1 nuclease
FT                   2. BglII linker 8bp cagatctg
FT                   -> pIJ2922 2700bp"
FT   -               1..403
FT                   /note="pUC18 1..403 403bp
FT                   HindIII = A^AGCT T
FT                   \                cagatctg"
FT   -               404..411
FT                   /note="cagatctg 8bp
FT                   \  cagatctg
FT                   HindIII = A^AGCTT"
FT   -               412..462
FT                   /note="pUC18 400..450 51bp
FT                   EcoRI =   G^AATTC
FT                   \           cagatctg"
FT   -               463..470
FT                   /note="cagatctg 8bp
FT                   \       cagatctg
FT                   EcoRI =   G^AATT C"
FT   -               471..2702
FT                   /note="pUC18 455..2686 2232bp"
FT   CDS             6..>83
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   modified alpha peptide; NCBI gi: 58016"
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SQ   Sequence 2702 BP; 671 A; 682 C; 688 G; 661 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctcagatct gagcttgcat
     gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgcagatctg cgtaatcatg
     gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaaag
     ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     tc
//