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LOCUS       CVU47627     3332 bp    DNA   circular  SYN       26-FEB-1996
DEFINITION  Cloning vector PinPoint Xa-2, protein expression vector,
            complete sequence.
ACCESSION   U47627
NID         g1200493
KEYWORDS    .
SOURCE      Cloning vector PinPoint Xa-2.
  ORGANISM  Cloning vector PinPoint Xa-2
            artificial sequence; cloning vectors.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Brady,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-1996) Melinda Brady, R&D, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
COMMENT
            NCBI gi: 1200493
FEATURES               Location/Qualifiers
     source          1..3332
                     /organism="Cloning vector PinPoint Xa-2"
     CDS             1..>386
                     /note="Biotin purification tag;  NCBI gi: 1200494"
                     /codon_start=1
                     /transl_table=11
                     /db_xref="PID:g1200494"
                     /translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
                     GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
                     KVLVKERDAVQGGQGLIKIGDLELIEG"
     misc_feature    262..264
                     /note="biotinylated lysine codon;  Biotin purification
                     tag"
     primer_bind     325..343
                     /note="PinPoint(TM) sequencing primer binding site"
     misc_feature    379..390
                     /note="Factor Xa protease recognition site"
     misc_feature    387..442
                     /note="multiple cloning site"
     promoter        447..469
                     /note="SP6 RNA polymerase promoter"
     primer_bind     452..470
                     /note="SP6 sequencing primer binding site"
     CDS             920..1780
                     /gene="ampR"
                     /note="NCBI gi: 1200495"
                     /codon_start=1
                     /function="ampicillin resistance"
                     /transl_table=11
                     /product="beta-lactamase"
                     /db_xref="PID:g1200495"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        2797..2819
                     /note="T7 RNA polymerase promoter"
     promoter        3203..3283
                     /note="tac promoter"
BASE COUNT      812 a    854 c    885 g    781 t
ORIGIN      
        1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
       61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
      121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
      181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
      241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
      301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
      361 atcggggatc tcgagctcat cgaaggtcgc gaaagcttca gctgggatcc ggtaccgata
      421 tcagatctcc cggggcggcc gcgatctggt tctatagtgt cacctaaatc gtatgtgtat
      481 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt ccatatggtg
      541 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      601 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      661 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      721 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      781 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      901 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      961 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1021 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1081 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1141 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1201 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1261 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1321 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1381 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1441 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1501 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1561 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1621 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1681 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1741 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1861 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2341 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2641 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2701 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2761 gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact atagggagac
     2821 cggcctcgag cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     2881 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     2941 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     3001 tgcgtccggc gtagaggatc gatccgggct tatcgactgc acggtgcacc aatgcttctg
     3061 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat
     3121 tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg
     3181 gttctggcaa atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt
     3241 ggaattgtga gcggataaca atttcacaca ggaaacagaa ttcccagctt ggctgcagaa
     3301 ccattccatt cgttgatccg ggagtaactc ac
//