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ID   PIRL19     preliminary; circular DNA; SYN; 2246 BP.
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AC   ATCC37233;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pIRL19 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pIRL19 from pBR322
RC   pIRL20 from pIRL19 & pBR329
RA   Roychoudhury R., Lam A.;
RT   "Isolation of a new plasmid pIRL19: its use in construction of
RT   small vectors and detection of specific sequence in a foreign
RT   DNA";
RL   Gene 25:161-166(1983).
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RN   [2]
RC   R300B or pRC137 from E. coli IncQ plasmids
RC   R300B or NTP2 from E. coli IncQ plasmids
RC   R300B or RSF1010 from E. coli IncQ plasmids
RC   R300B or R1162 from E. coli IncQ plasmids
RC   R300B::Tn3 from R300B & R1drd-19, amp gene
RC   pTB86 from R300B & pACYC184
RC   pTB90, pTB91, pTB92 from R300B & pMK20, kan gene
RC   pGSS6 from pBR322 & R300B
RC   pGSS8, pGSS9 from pGSS6
RC   pGSS15 from pBR322 & pGSS8
RC   [pBR322 or pRC400 from pBR313]
RC   [pBR329 or pRC922 from pBR328]
RA   Barth P.T., Tobin L., Sharpe G.S.;
RT   "Development of broad host-range plasmid vectors";
RL   Molecular Biology Pathogen Ecol of Bacterial Plasmids 0:439-448(1981).
RL   Levy S.B., Clowes R.C., Koenig E.L., Plenum, New York.
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RN   [3]
RC   pRP301 from RP4
RA   Windass J.D., Worsey M.J., Pioli E.M., Pioli D., Barth P.T.,
RA   Atherton K.T., Dart E.C., Byrom D., Powell K., Senior P.J.;
RT   "Improved conversion of methanol to single-cell protein by
RT   Methylophilus methylotrophus";
RL   Nature 287:396-401(1980).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Plasmid for constructing very small plasmid vectors.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pIRL19)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HaeII-HaeII 370bp 2352..2722, ori
FT                   2. pBR322 HaeII-HaeII 1876bp 2722..4361..237,
FT                   \ amp gene/1875bp
FT                   -> pIRL19 2245bp"
FT   -               1..370
FT                   /note="pBR322 2352..2721 370bp
FT                   HaeII = RGCGC^Y"
FT   -               371..2246
FT                   /note="pBR322 2722..4361..236 1876bp
FT                   HaeII = RGCGC^Y"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV-EcoRI-HindIII-ClaI-HgiE11-
FT                   NspcI-HaeIII(400)-FnuDII(984)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 2246 BP; 566 A; 571 C; 531 G; 578 T; 0 other;
     tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     cgtatcacga ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa
     gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     cagtcactat ggcgtgctgc tagcgc
//