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ID   PIVX       preliminary; circular DNA; SYN; 902 BP.
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AC   L08918; VB0010; V00089; X14353; ATCC39083;
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DT   07-FEB-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pi-VX - complete.
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KW   cloning vector; origin of replication; plasmid; polylinker;
KW   suppressor gene; suppressor transfer RNA.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-902
RC   plink322 from pBR322
RA   Seed B.;
RT   "Purification of genomic sequences from bacteriophage libraries
RT   and selection in vivo";
RL   Molecular Cloning Laboratory Manual 0:353-361(1982).
RL   Maniatis T., Fritsch E., Sambrook J., eds.,
RL   Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
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RN   [2]
RP   1-902
RC   pTF487 from R6K
RC   pTF128U from pNOP42 & ColE1
RC   pTF128G or pMM7 from pNOP42
RC   pTF48 from pGC8
RC   pTF124U/128G, pLY126U/128G from pTF128G
RA   Fitzwater T., Yang Y.L., Zhang X.Y., Polisky B.;
RT   "Mutations affecting RNA-DNA hybrid formation of the colE1
RT   replication primer RNA. Restoration of RNA I sensitivity to a
RT   copy-number mutant by second-site mutations";
RL   J. Mol. Biol. 226:997-1008(1992).
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RN   [3]
RP   1-902
RC   p793 from pKB413 & pRD69
RC   p3 from pLM2 & phage PRD1
RC   plasmid from pBR322 & pGA46 & linker
RC   plasmid2 from plasmid & pACYC177
RC   piV1 from plasmid2 & plasmid
RC   piBP from piV1
RC   piBHg from piV1
RC   piVX from p793 & piV1
RC   [piSV from SV40]
RA   Seed B.;
RT   "Purification of genomic sequences from bacteriophage libraries by
RT   recombination and selection in vivo";
RL   Nucleic Acids Res. 11:2427-2445(1983).
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RN   [4]
RP   1-902
RC   piVX
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [5]
RC   pTF487 from pGC8
RC   pSTK131 from pNOP42
RC   pTF128C, pTF128G, pTF131A from pSTK131
RC   pMM1, pMM4, pMM7 from pSTK131
RC   pEW2705 from pSTK131
RA   Polisky B., Zhang X.Y., Fitzwater T.;
RT   "Mutations affecting primer RNA interaction with the replication
RT   repressor RNA I in plasmid ColE1: potential RNA folding pathway
RT   mutants";
RL   EMBO J. 9:295-304(1990).
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RN   [6]
RC   pGC8 from pBR322 & ps1EMBL, R6K ori
RC   phage phiBG46 from pBR322 & ps1EMBL
RC   phage phiBG34 from pBR322 & ps1EMBL
RA   Cesareni G., Cornelissen M., Lacatena R.M., Castignoli L.;
RT   "Controll of pMB1 replication: inhibition of primer formation by
RT   Rop requires RNA I";
RL   EMBO J. 3:1365-1369(1984).
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RN   [7]
RC   piVX from p793 & piV1
RA   Neve R.L., Bruns G.A., Dryja T.P., Kurnit D.M.;
RT   "Retrieval of human DNA from rodent-human genomic libraries by a
RT   recombination process";
RL   Gene 23:343-354(1983).
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CC   X14353 is old 1-902 sequence.
CC
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Cloning vector used for screening bacteriophage lambda
CC   gene libraries for specific DNA sequences in Escherichia coli.
CC   The origin of replication is derived from pMB1 via pBR345 and
CC   pKB413. The sequence given for pBR345 differs at one position from
CC   that of pBR322. This may be a sequencing error.
CC   Probe sequences are inserted in the vector pi-VX.
CC   Contains a polylinker, an origin of replication (from pMB1), and
CC   tyrosine amber-suppressor gene (synthetic supF gene).
CC   old ATCC39083 contains piVX in combination with plasmid p3.
CC   NCBI gi: 58159 & 58268 & 310786
CC   NM (pi-VX)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(lambda screening)
CC   SE ()
CC   PA (pBR322)(EcoTgy)(pMB1)
CC   BR ()
CC   OF (piAN7)(mWB2344)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMB1, ori
FT                   -> pKB413 590bp
FT                   1. pBR322
FT                   2. tyrosine tRNA suppressor
FT                   -> pRD69 203bp
FT                   1. pKB413 590bp, pMB1 ori
FT                   2. pRD69 203bp, tyrosine tRNA suppressor
FT                   -> p793 793bp
FT                   1. RP1
FT                   2. suppressor gene
FT                   -> pLM2
FT                   1. pLM2
FT                   spontaneous mutation to tra-
FT                   -> p3
FT                   1. pBR322 BamHI-HindIII 4015bp 376..4361..30
FT                   2. pGA46 MstI
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   HindIII-BamHI
FT                   -> plasmid [pGA46 BglII, pGA46 PstI]
FT                   1. plasmid SalI-SalI
FT                   Klenow:Klenow
FT                   BamHI linker 6bp ggatcc:BamHI linker 6bp ggatcc
FT                   remove BamHI-BamHI 6bp
FT                   EcoRI methylase
FT                   SalI-SalI
FT                   Klenow:Klenow
FT                   EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT                   -> plasmid2 [no SalI]
FT                   1. plasmid2 BamHI-BamHI, MCS
FT                   2. pACYC177 BamHI 3941bp 3321..3321, tet gene
FT                   -> plasmid3
FT                   1. plasmid3 EcoRI, linker
FT                   2. p793 EcoRI 793bp, complete
FT                   -> plasmid4
FT                   1. plasmid4 remove BamHI-BamHI, MCS
FT                   -> piV1
FT                   1. piV1 remove HindIII-BglII 100bp, MCS
FT                   Klenow:Klenow
FT                   XbaI linker 6bp tctaga
FT                   -> piVX 902bp"
FT   misc_binding    1..115
FT                   /note="MCS EcoRI-ClaI-HindIII-XbaI-BglII-
FT                   PstI-BamHI"
FT   misc_binding    1..115
FT                   /note="MCS unique ClaI-HindIII-XbaI-BglII-PstI-BamHI"
FT   misc_feature    4..37
FT                   /note="from pBR322"
FT   tRNA            110..212
FT                   /note="RNA E. coli suppressor tRNA; E. coli SupF"
FT   tRNA            111..317
FT                   /note="RNA E. coli tyr-tRNA synthetic;
FT                   GenBank(50): EcoTgy"
FT   rep_origin      320..901
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322);
FT                   from pBR322; origin at 600"
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SQ   Sequence 902 BP; 192 A; 250 C; 237 G; 223 T; 0 other;
     gaattctcat gtttgacagc ttatcatcga taagcttcta gagatcttcc atacctacca
     gttctccgcc tgcagcaatg gcaacaacgt tgcccggatc cggtcgcgcg aattctttcg
     gacttttgaa agtgatggtg gtgggggaag gattcgaacc ttcgaagtcg atgacggcag
     atttagagtc tgctcccttt ggccgctcgg gaaccccacc acgggtaatg cttttactgg
     cctgctccct tatcgggaag cggggcgcat catatcaaat gacgcgccgc tgtaaagtgt
     tacgttgaga aagaattcgg cgttgctggc gtttttccat aggctccgcc cccctgacga
     gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     atttggtatc tgcgctctgc tgaagccagt tccttcggaa aaagagttgg tagctcttga
     tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     cc
//