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ID   PJB61      preliminary; circular DNA; SYN; 5557 BP.
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AC   ATCC39162;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pJB61 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJB61 from pBR322 & gene III
RA   ;
RT   ;
RL   Unpublished (1986).
RL   U.S. Patent No. 4,595,658 dated June 17, 1986.
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   The insert extends from the HaeIII site at 1.4 kb on the map to the
CC   AluI site at 2.96 kb.
CC   This was constructed in a two-step process. The 5' HaeII site was
CC   linkered with EcoRI; the 3' AluI site was linkered with BamHI.
CC   The insert contains an internal BamHI site about 0.8 kb from the EcoRI
CC   site.
CC   The expression of the gene III product facilitates externalization of
CC   periplasmic protein by perturbing the permeability of the outer
CC   membrane.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pJB61)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli K-38)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(M13)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-BamHI 377bp
FT                   \ 4360..4361..376, 3984bp
FT                   2. M13 HaeIII-AluI 1567bp 1398..2965, gene III
FT                   \ AluI = 41 65 205 231 335 936 1490 1519 2965 3278
FT                   \ 3614 4098 5428 5632 5889 6109 6136 6337
FT                   \ HaeIII = 1398 2247 2556 5083 5241 5347 5416 5727
FT                   \ 5869 6182
FT                   EcoRI linker 6bp gaattc:BamHI linker 6bp ggatcc
FT                   -> pJB61 5557bp"
FT   -               1..3984
FT                   /note="pBR322 376..4359 3984bp
FT                   EcoRI = G^AATTC
FT                   \         aattc"
FT   -               3985..3989
FT                   /note="aattc 5bp
FT                   \     aattc
FT                   HaeIII = GG^CC"
FT   -               3990..5556
FT                   /note="M13 1398..2964 1567bp
FT                   AluI = AG^CT
FT                   \         g"
FT   -               5557..5557
FT                   /note="g 1bp
FT                   \       g
FT                   BamHI = G^GATCC"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI-PvuII-HincII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 5557 BP; 1270 A; 1431 C; 1418 G; 1438 T; 0 other;
     gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
     caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg
     cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
     gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
     gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag
     ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagaattcc ctttaactcc ctgcaagcct cagcgaccga
     atatatcggt tatgcgtggg cgatggttgt tgtcattgtc ggcgcaacta tcggtatcaa
     gctgtttaag aaattcacct cgaaagcaag ctgataaacc gatacaatta aaggctcctt
     ttggagcctt tttttttgga gattttcaac gtgaaaaaat tattattcgc aattccttta
     gttgttcctt tctattctca ctccgctgaa actgttgaaa gttgtttagc aaaaccccat
     acagaaaatt catttactaa cgtctggaaa gacgacaaaa ctttagatcg ttacgctaac
     tatgagggtt gtctgtggaa tgctacaggc gttgtagttt gtactggtga cgaaactcag
     tgttacggta catgggttcc tattgggctt gctatccctg aaaatgaggg tggtggctct
     gagggtggcg gttctgaggg tggcggttct gagggtggcg gtactaaacc tcctgagtac
     ggtgatacac ctattccggg ctatacttat atcaaccctc tcgacggcac ttatccgcct
     ggtactgagc aaaaccccgc taatcctaat ccttctcttg aggagtctca gcctcttaat
     actttcatgt ttcagaataa taggttccga aataggcagg gggcattaac tgtttatacg
     ggcactgtta ctcaaggcac tgaccccgtt aaaacttatt accagtacac tcctgtatca
     tcaaaagcca tgtatgacgc ttactggaac ggtaaattca gagactgcgc tttccattct
     ggctttaatg aggatccatt cgtttgtgaa tatcaaggcc aatcgtctga cctgcctcaa
     cctcctgtca atgctggcgg cggctctggt ggtggttctg gtggcggctc tgagggtggt
     ggctctgagg gtggcggttc tgagggtggc ggctctgagg gaggcggttc cggtggtggc
     tctggttccg gtgattttga ttatgaaaag atggcaaacg ctaataaggg ggctatgacc
     gaaaatgccg atgaaaacgc gctacagtct gacgctaaag gcaaacttga ttctgtcgct
     actgattacg gtgctgctat cgatggtttc attggtgacg tttccggcct tgctaatggt
     aatggtgcta ctggtgattt tgctggctct aattcccaaa tggctcaagt cggtgacggt
     gataattcac ctttaatgaa taatttccgt caatatttac cttccctccc tcaatcggtt
     gaatgtcgcc cttttgtctt tagcgctggt aaaccatatg aattttctat tgattgtgac
     aaaataaact tattccgtgg tgtctttgcg tttcttttat atgttgccac ctttatgtat
     gtattttcta cgtttgctaa catactgcgt aataaggagt cttaatcatg ccagttcttt
     tgggtattcc gttattattg cgtttcctcg gtttccttct ggtaactttg ttcggctatc
     tgcttacttt tcttaaaaag ggcttcggta agatagg
//