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ID   PJBD41     preliminary; circular DNA; SYN; 7533 BP.
XX
AC   IG9894;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/E.coli plasmid vector pJBD41 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMP78-1, pMP80-3 from pBR325 & pJDB219, yeast 2 micron/LEU2 gene
RC   pMP81 from pCR1 & pJDB219, yeast 2 micron/LEU2 gene
RA   Hollenberg C.P.;
RT   "The expression in Saccharomyces cerevisiae of bacterial
RT   beta-lactamase and other antibiotic resistance genes integrated in
RT   a 2 micron DNA vector";
RL   ICN-UCLA Symp. Mol. Cell. Biol. [Extrachrom DNA] 15:325-338(1979).
RL   Cummings D.J., Borst P., Dawid I.B., Weissman S.M., Fox C.F.;
RL   Academic Press, New York.
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJBD41)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli JA221)(Saccharomyces cerevisiae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pMB9)(yeast 2 micron)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMB9 EcoRI 5295bp 5294..5294, 5' to tet gene
FT                   2. yeast 2 micron B form EcoRI-EcoRI 2235bp
FT                   \ 2408..3942 & 703..2
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   -> pJBD41 7523bp [tet gene]"
FT   -               1..5293
FT                   /note="pMB9 1..5293 5293bp
FT                   EcoRI = G^AATTC"
FT   -               5294..6828
FT                   /note="yeast 2 micron 2408..3942 1535bp"
FT   -               6829..7531
FT                   /note="yeast 2 micron 1..703 703bp complement
FT                   EcoRI = G^AATTC"
FT   -               7532..7533
FT                   /note="pMB9 5294..5295 2bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
XX
SQ   Sequence 7533 BP; 1868 A; 1745 C; 1943 G; 1977 T; 0 other;
     ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
     aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
     aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
     ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
     ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
     ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
     ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
     ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
     ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
     gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
     acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
     ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
     ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
     ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
     cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
     gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc
     attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa
     tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt
     cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct
     cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
     aagcgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag
     cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
     cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc
     gagaggatgc tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt
     gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt
     tccggaaaac tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat
     aacctgaatc agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt
     caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggctgtc
     agggaacagg gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag
     tggtccggtt gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca
     gggtataccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac
     gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat
     ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg
     ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct
     acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc
     ttggcagacg ctgttaaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg
     caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt
     cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat
     tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat
     tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt
     tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa
     tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg
     agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa
     aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg
     cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg
     ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg
     aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga
     acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga
     aagagtgggt aatggaactt ggatgaagaa agagtgaaca tgatggttcc gccatggggc
     gggcggtcat ggagttgtca ctggcagatt tacctatgac ccagcaaaac atcatcgaca
     agctggaacg gtaccggaag gaaacgggaa acgtgatagg taagggtgtg aacgggatgc
     agctgagata gtcaggaagg gtagtaaagc tgtgaagtag gcacaaaaaa cccggcgcgg
     tggccggttg ttaaagctca atatcaacta tgaaaagttt actaatagcc ccttttctaa
     ctgctgcttt ggcagttact tttaccgctg actcagtact tagacccgtt gacagaaaac
     gactaagtga aatgagatac gggttgtctg cctttgaagc agatggatcg cttatttgtg
     ctgagatgcg cttatctgaa tcatcacctt ctataatgac tttagcggtc attcggtgag
     cgtcgaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt agtttatcac
     agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg
     gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
     cgttgggtcc tggaattcca cggactatag actatactag tatactccgt ctactgtacg
     atacacttcc gctcaggtcc ttgtccttta acgaggcctt accactcttt tgttactcta
     ttgatccagc tcagcaaagg cagtgtgatc taagattcta tcttcgcgat gtagtaaaac
     tagctagacc gagaaagaga ctagaaatgc aaaaggcact tctacaatgg ctgccatcat
     tattatccga tgtgacgctg cagcttctca atgatattcg aatacgcttt gaggagatac
     agcctaatat ccgacaaact gttttacaga tttacgatcg tacttgttac ccatcattga
     attttgaaca tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat
     aataatatat agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa
     ctattgcatc tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt
     tccatcttgc acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag
     aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
     tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
     ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
     tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
     ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
     ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
     taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa
     agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
     tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
     gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
     tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
     gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg
     tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
     gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatacag aataggaact
     tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc
     tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata
     tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct
     atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg
     gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat
     tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat atgcatagta
     ccgagaaact agtgcgaagt agtgatcagg tattgctgtt atctgatgag tatacgttgt
     cctggccacg gcagaagcac gcttatcgct ccaatttccc acaacattag tcaactccgt
     taggcccttc attgaaagaa atgaggtcat caaatgtctt ccaatgtgag attttgggcc
     attttttata gcaaagattg aataaggcgc atttttcttc aaagctttat tgtacgatct
     gactaagtta tcttttaata attggtattc ctgtttattg cttgaagaat tgccggtcct
     atttactcgt tttaggactg gttcagaatt caa
//