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ID   PJH101         preliminary; circular DNA; SYN; 5391 BP.
XX
AC   IG9858;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pJH101 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pLS23 from pLS14
RC   pLS80 from pUC8 & pLS50
RC   pLS30 from pJH101 & pLS80
RC   pLS51 from pLS50
RC   pLS52 from pLS51
RC   pLS151, pLS152 from pLS51
RC   pLS301 from pUC8 & pLS52, B.subtilis sacB gene
RC   pLS302 from pLS301 & pLS51
RC   pLS308, pLS310 from pBR322 & pLS301
RA   Steinmetz M., Le Coq D., Aymerich S., Gonzy-Treboul G., Gay P.;
RT   "The DNA sequence of the gene for the secreted Bacillus subtilis
RT   enzyme levansucrase and its genetic control sites";
RL   Mol. Gen. Genet. 200:220-228(1985).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJH101)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pC194)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 PvuII 4361bp 2067..2067
FT                   2. pC194 HpaII-MboI 1030bp 975..2005, cat gene
FT                   -> pJH101 5400bp"
FT   -               1..2066
FT                   /note="pBR322 1..2066 2066bp
FT                   PvuII =       CAG^CTG
FT                   HpaII = HapII = C^CGG"
FT   -               2067..3096
FT                   /note="pC194 975..2004 1030bp
FT                   MboI = Sau3AI = ^GATC
FT                   PvuII =      CAG^CTG"
FT   -               3097..5391
FT                   /note="pBR322 2067..4361 2295bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5391 BP; 1346 A; 1363 C; 1296 G; 1386 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagggca atagttaccc ttattatcaa gataagaaag
     aaaaggattt ttcgctacgc tcaaatcctt taaaaaaaca caaaagacca cattttttaa
     tgtggtcttt attcttcaac taaagcaccc attagttcaa caaacgaaaa ttggataaag
     tgggatattt ttaaaatata tatttatgtt acagtaatat tgacttttaa aaaaggattg
     attctaatga agaaagcaga caagtaagcc tcctaaattc actttagata aaaatttagg
     aggcatatca aatgaacttt aataaaattg atttagacaa ttggaagaga aaagagatat
     ttaatcatta tttgaaccaa caaacgactt ttagtataac cacagaaatt gatattagtg
     ttttataccg aaacataaaa caagaaggat ataaatttta ccctgcattt attttcttag
     tgacaagggt gataaactca aatacagctt ttagaactgg ttacaatagc gacggagagt
     taggttattg ggataagtta gagccacttt atacaatttt tgatggtgta tctaaaacat
     tctctggtat ttggactcct gtaaagaatg acttcaaaga gttttatgat ttataccttt
     ctgatgtaga gaaatataat ggttcgggga aattgtttcc caaaacacct atacctgaaa
     atgctttttc tctttctatt attccatgga cttcatttac tgggtttaac ttaaatatca
     ataataatag taattacctt ctacccatta ttacagcagg aaaattcatt aataaaggta
     attcaatata tttaccgcta tctttacagg tacatcattc tgtttgtgat ggttatcatg
     caggattgtt tatgaactct attcaggaat tgtcagatag gcctaatgac tggcttttat
     aatatgagat aatgccgact gtacttttta cagtcggttt tctaatgtca ctaacctgcc
     ccgttagttg aagaaggttt ttatattaca gctccactgc ctcgcgcgtt tcggtgatga
     cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a
//