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ID   PJMSP0     preliminary; circular DNA; SYN; 9782 BP.
XX
AC   IG9827;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pJMSP-0 - complete, MCS & SP6 polymerase.
XX
KW   cloning vector; polylinker.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-51
RC   pJMSP-0 from pGEM-3 & M13mp19
RC   pJMSP-8 from pJMSP-0
RA   Bujarski J.J., Kaesberg P.;
RT   "DNA inserted two bases down from the initiation site of a SP6
RT   polymerase transcription vector is transcribed efficiently in vitro";
RL   Nucleic Acids Res. 15:1337-1337(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJMSP-8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pGEM-3)(M13mp19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3 SacI-NaeI 2538bp 57..2595, Sp6 prom
FT                   2. M13mp19 remove AvaI-SacI 14bp 6270..6284,
FT                   \ MCS/7236bp
FT                   -> pJMSP-0 9774bp"
FT   -               1..6273
FT                   /note="M13mp19 1..6273 6273bp
FT                   AvaI =   C^YCGR G
FT                   NaeI =      GCC^GGC"
FT   -               6274..8815
FT                   /note="pGEM-3 53..2594 2542bp complement
FT                   SacI = SstI = GAGCT^C"
FT   -               8816..9782
FT                   /note="M13mp19 6284..7250 967bp"
FT   misc_feature    1..51
FT                   /note="MCS StuI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-
FT                   SacI-EcoRI"
FT   misc_feature    1..5
FT                   /note="E. coli pJMSP-8 vector sequence"
FT   misc_feature    4..4
FT                   /note="E. coli mRNA transcription start"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
XX
SQ   Sequence 9782 BP; 2379 A; 2180 C; 2189 G; 3034 T; 0 other;
     aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
     atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
     cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
     gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
     tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
     ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
     tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
     cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
     tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
     aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
     ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
     aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
     atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
     tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
     caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
     ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
     aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
     tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
     ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
     tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
     caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa
     gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
     ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
     gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
     tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
     tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
     ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
     ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
     ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
     cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
     cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
     tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
     acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
     aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
     cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
     tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
     gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
     actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
     tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
     aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
     tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
     gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
     cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
     agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
     attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
     tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
     tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
     tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
     tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
     taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
     tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
     ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
     gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
     tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
     aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
     tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
     cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
     agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
     tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
     tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
     tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
     tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
     cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
     aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
     atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
     acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct
     ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
     catgcctgca ggtcgactct agaggatccc cggggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     gctgcttcct aatgcaggag tcgcataagg gagagcgtcg atatggtgca ctctcagtac
     aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     cagctggctt atcgaaatta atacgactca ctatagggag accggaattc gagctcgaat
     tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tacggtcgtc
     gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat
     cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
     acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
     gttcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg gggcttttct
     gattatcaac cggggtacat atgattgaca tgctagtttt acgattaccg ttcatcgatt
     ctcttgtttg ctccagactc tcaggcaatg acctgatagc ctttgtagat ctctcaaaaa
     tagctaccct ctccggcatt aatttatcag ctagaacggt tgaatatcat attgatggtg
     atttgactgt ctccggcctt tctcaccctt ttgaatcttt acctacacat tactcaggca
     ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt
     ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta gctttatgct
     ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat ttattggatg
     tt
//