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ID   PJMSP8     preliminary; circular DNA; SYN; 9775 BP.
XX
AC   X05042;
XX
DT   29-NOV-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pJMSP-8 - complete, MCS & SP6 polymerase.
XX
KW   cloning vector; polylinker.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-51
RC   pJMSP-0 from pGEM-3 & M13mp19
RC   pJMSP-8 from pJMSP-0
RA   Bujarski J.J., Kaesberg P.;
RT   "DNA inserted two bases down from the initiation site of a SP6
RT   polymerase transcription vector is transcribed efficiently in vitro";
RL   Nucleic Acids Res. 15:1337-1337(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NCBI gi: 58125
CC   NM (pJMSP-8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pGEM-3)(M13mp19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3 SacI-NaeI 2538bp 57..2595, Sp6 prom
FT                   2. M13mp19 remove AvaI-SacI 14bp 6270..6284,
FT                   \ MCS/7236bp
FT                   -> pJMSP-0 9774bp
FT                   1. pJMSP-0 9774bp, MCS
FT                   S1 nuclease, makes StuI [AGG^CCT]
FT                   -> pJMSP-8 9800bp"
FT   -               1..6236
FT                   /note="M13mp19 1..6236 6236bp
FT                   \ ...ag
FT                   \      gcct..."
FT   -               6237..6266
FT                   /note="M13mp19 6244..6273 30bp
FT                   AvaI =   C^YCGR G
FT                   NaeI =      GCC^GGC"
FT   -               6267..8808
FT                   /note="pGEM-3 53..2594 2542bp complement
FT                   SacI = SstI = GAGCT^C"
FT   -               8809..9775
FT                   /note="M13mp19 6284..7250 967bp"
FT   misc_feature    1..51
FT                   /note="MCS StuI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-
FT                   SacI-EcoRI"
FT   misc_feature    1..5
FT                   /note="E. coli pJMSP-8 vector sequence"
FT   misc_feature    4..4
FT                   /note="E. coli mRNA transcription start"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
XX
SQ   Sequence 9775 BP; 2378 A; 2178 C; 2188 G; 3031 T; 0 other;
     aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
     atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
     cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
     gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
     tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
     ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
     tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
     cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
     tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
     aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
     ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
     aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
     atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
     tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
     caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
     ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
     aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc
     tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca
     tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt
     ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca
     tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact
     caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa
     gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt
     ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt
     gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct
     tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg
     tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt
     tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc
     ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg
     ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact
     ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc
     tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg
     attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg
     ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat
     cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt
     cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat
     tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat
     acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt
     aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg
     cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct
     tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat
     gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat
     actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat
     tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta
     aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt
     tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg
     gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct
     cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat
     agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc
     attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt
     tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt
     tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg
     tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc
     tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta
     taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga
     tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac
     ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa
     gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt
     tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc
     aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga
     tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg
     cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt
     agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg
     tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat
     tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg
     tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt
     tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact
     cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa
     aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt
     atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa
     acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
     ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct
     ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaaggcct
     gcaggtcgac tctagaggat ccccggggca tcaccggcgc cacaggtgcg gttgctggcg
     cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     ccttgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     cctaatgcag gagtcgcata agggagagcg tcgatatggt gcactctcag tacaatctgc
     tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     cttatcgaaa ttaatacgac tcactatagg gagaccggaa ttcgagctcg aattcactgg
     ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
     cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatacggtc gtcgtcccct
     caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta
     cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
     atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttccta
     ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt tctgattatc
     aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg attctcttgt
     ttgctccaga ctctcaggca atgacctgat agcctttgta gatctctcaa aaatagctac
     cctctccggc attaatttat cagctagaac ggttgaatat catattgatg gtgatttgac
     tgtctccggc ctttctcacc cttttgaatc tttacctaca cattactcag gcattgcatt
     taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg cttctcccgc
     aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat gctctgaggc
     tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg atgtt
//