back Return to this vector's summary.
ID   PJRD158    preliminary; circular DNA; SYN; 3903 BP.
XX
AC   X01453; M12802;
XX
DT   15-MAR-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pJRD158 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3903
RC   pJRD158 from pBR322 & pBR327
RA   Huesterspreute M., Davison J.;
RT   "Restriction site bank vectors. II. DNA sequence analysis of
RT   plasmid pJRD158";
RL   DNA 3:259-268(1984).
XX
RN   [2]
RC   pJRD158
RA   Davison J.;
RT   ;
RL   Submitted (01-JUL-1987) to GenBank on computer-readable media by:
RL   Davison J., .
XX
CC   NM (pJRD158)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (restriction site bank)
CC   SE ()
CC   PA (pBR327)(T5)(pKC7)
CC   BR (pJRD215)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI-TaqI 1270bp 4360..4361..1269,
FT                   \ tet gene
FT                   2. T5 HindIII-HindIII 177bp, L fragment
FT                   3. pKC7 remove HpaI(1)-HpaI(4) [? no HpaI]
FT                   BclI-PstI 212bp 311..523, kan gene [162bp]
FT                   4. T5 EcoRI-AvaI
FT                   Sau3AI/MboI-Sau3AI/MboI 80bp,
FT                   5. T5 remove XbaI-XbaI
FT                   BAL31 nuclease
FT                   HindIII-HindIII 177bp, L fragment
FT                   6. pKC7 XhoI-HincII 92bp 1623..1715,
FT                   \ 5' to kan gene [88bp]
FT                   7. pBR327 AvaI 3273bp 1425..1425, ori/amp gene
FT                   XhoI linker 6bp ctcgag
FT                   -> pJRD158 3903bp"
FT   misc_feature    1..1268
FT                   /note="from pBR322"
FT   CDS             86..1273
FT                   /note="ANT E. coli tetracycline resistance gene (tet),
FT                   from pBR322"
FT   misc_recomb     1268..1269
FT                   /note="pBR322 DNA end/bacteriophage T5 DNA start"
FT   misc_feature    1269..1443
FT                   /note="bacteriophage T5 Hind III 2 fragment"
FT   misc_recomb     1443..1444
FT                   /note="bacteriophage T5 DNA end/pKC7 DNA start"
FT   misc_feature    1444..1524
FT                   /note="from pKC7, E. coli kanamycin resistance
FT                   gene fragment"
FT   misc_recomb     1524..1525
FT                   /note="pKC7 DNA end/bacteriophage T5 DNA start"
FT   misc_feature    1525..1604
FT                   /note="bacteriophage T5 EcoRI-AvaI fragment"
FT   misc_recomb     1604..1605
FT                   /note="bacteriophage T5 DNA end/pKC7 DNA start"
FT   misc_feature    1605..1609
FT                   /note="pKC7 Bgl II site remnant"
FT   misc_recomb     1609..1610
FT                   /note="pKC7 DNA end/bacteriophage T5 DNA start"
FT   misc_feature    1610..1960
FT                   /note="bacteriophage T5 Hind III L fragment"
FT   misc_recomb     1960..1961
FT                   /note="bacteriophage T5 DNA end/pKC7 DNA start"
FT   misc_feature    1961..2047
FT                   /note="pKC7 Hinc II-Xho I fragment"
FT   misc_feature    2048..2054
FT                   /note="remnant of Xho linker"
FT   misc_recomb     2048..2049
FT                   /note="pKC7 DNA end/pBR322 start"
FT   misc_feature    2055..3903
FT                   /note="from pBR327, ori and ampR gene"
FT   rep_origin      2075..2075
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   mat_peptide     complement(2838..3626)
FT                   /note="E. coli beta-lactamase mature peptide"
FT   CDS             complement(2835..3698)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   sig_peptide     complement(3627..3695)
FT                   /note="E. coli beta-lactamase signal peptide"
XX
SQ   Sequence 3903 BP; 899 A; 1041 C; 955 G; 1008 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctac tgaagtggat ttctttaaga gctcctttaa cttcctcacc agtagttgta
     tcggtaccat aagtagaagc agcaacccaa gtagctttac cagcatccgg ttcaaccagc
     atagtaagaa tcttactgga catcggcagt tcttcgaaca gtgcgccaac taccagctct
     ttctgcagtt cattcagggc accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     gaaacgatcc tcatcctgtc tcttgatcgc attctaacgc gtttaatttt gatggggatt
     aaaacaaagg gggatttaac tggaggcgtg aaaaacgggg agctgatcag atctttaaaa
     gaggatattt gggagtacgg tttagctttc ataccaccat ctagagtctt tgttttgacg
     ccattagcgt acgtaacaat attatattta tctccgacga tattctcatc agtgaaatcc
     agctggagtt ctttagcaaa tttttttatt agctgagata tagtatgata gtgatgaagt
     tctaggtctt cactactacc acatatagca cagcaaccat ccttactata cctagatttt
     ataccatccc tcattatgga aatggcatca cgtttgtact tagtattagt ggccatactc
     ctccaatcca aagctattta gaaagattac tatatcctca aacaggcggt aaccggcctc
     ttcatcggga atgcgcgcga ccttcagcat cgccggcatg tccccctggc ggacgggaag
     tatccagctc gaggtcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tgatagctct
     tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     cggcgaccga gttgctcttg cccgacgtca atacgggata ataccgcgcc acatagcaga
     actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     gaa
//