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ID   PJRD171    preliminary; circular DNA; SYN; 3941 BP.
XX
AC   IG9882;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Gram-negative bacteria plasmid vector pJRD171 - complete, phase.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJRD177 from pJRD173 & pJRD182
RC   pJRD178 from pJRD177 & pEMBL8
RC   pJRD179 from pJRD178 & pIC20R
RC   pJRD187 from pJRD179 & pCQV2
RC   pJRD187K from pJRD187 & pKMH4-10
RC   pJRD187Ka, pJRD187Kg from pJRD187 & pKMH4-10
RC   pJRD187Kc from pJRD187 & pKMH4-10
RC   pJRD215K from pJRD187K & pJRD215
RA   Davison J., Heusterspreute M., Chevalier N., Brunel F.;
RT   "A phase-shift fusion system for the regulation of foreign gene
RT   expression by lambda repressor in Gram-negative bacteria";
RL   Gene 60:227-235(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJRD171)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Gram-negative bacteria)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pJRD158)(yeast 2 micron)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pJRD158 BclI 3903bp 1605..1605
FT                   2. yeast 2 micron BclI-Sau3A 38bp 305..343,
FT                   \ unique NsiI-NdeI
FT                   -> pJRD171 3941bp"
FT   -               1..1604
FT                   /note="pJRD158 1..1604 1604bp
FT                   BclI =  T^GATCA"
FT   -               1605..1642
FT                   /note="yeast 2 micron 305..342 38bp
FT                   BclI =         T^GATCA
FT                   MboI = Sau3AI = ^GATC"
FT   -               1643..3941
FT                   /note="pJRD158 1605..3903 2299bp"
FT   rep_origin      0..0
FT                   /note="ORI Gram negative bacteria"
XX
SQ   Sequence 3941 BP; 908 A; 1051 C; 961 G; 1021 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctac tgaagtggat ttctttaaga gctcctttaa cttcctcacc agtagttgta
     tcggtaccat aagtagaagc agcaacccaa gtagctttac cagcatccgg ttcaaccagc
     atagtaagaa tcttactgga catcggcagt tcttcgaaca gtgcgccaac taccagctct
     ttctgcagtt cattcagggc accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     gaaacgatcc tcatcctgtc tcttgatcgc attctaacgc gtttaatttt gatggggatt
     aaaacaaagg gggatttaac tggaggcgtg aaaaacgggg agctgatcac tacttcgcac
     tagtttctcg gtactatgca tagatcagat ctttaaaaga ggatatttgg gagtacggtt
     tagctttcat accaccatct agagtctttg ttttgacgcc attagcgtac gtaacaatat
     tatatttatc tccgacgata ttctcatcag tgaaatccag ctggagttct ttagcaaatt
     tttttattag ctgagatata gtatgatagt gatgaagttc taggtcttca ctactaccac
     atatagcaca gcaaccatcc ttactatacc tagattttat accatccctc attatggaaa
     tggcatcacg tttgtactta gtattagtgg ccatactcct ccaatccaaa gctatttaga
     aagattacta tatcctcaaa caggcggtaa ccggcctctt catcgggaat gcgcgcgacc
     ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga ggtcgggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg atagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cgacgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a
//