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ID   PJRD173    preliminary; circular DNA; SYN; 3898 BP.
XX
AC   IG9880;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Gram-negative bacteria plasmid vector pJRD173 - complete, phase.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJRD177 from pJRD173 & pJRD182
RC   pJRD178 from pJRD177 & pEMBL8
RC   pJRD179 from pJRD178 & pIC20R
RC   pJRD187 from pJRD179 & pCQV2
RC   pJRD187K from pJRD187 & pKMH4-10
RC   pJRD187Ka, pJRD187Kg from pJRD187 & pKMH4-10
RC   pJRD187Kc from pJRD187 & pKMH4-10
RC   pJRD215K from pJRD187K & pJRD215
RA   Davison J., Heusterspreute M., Chevalier N., Brunel F.;
RT   "A phase-shift fusion system for the regulation of foreign gene
RT   expression by lambda repressor in Gram-negative bacteria";
RL   Gene 60:227-235(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJRD173)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Gram-negative bacteria)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pJRD158)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pJRD158 BclI-BglII 3898bp 1610..3903..1605
FT                   -> pJRD173 3898bp [BclI-BglII]"
FT   -               1..1604
FT                   /note="pJRD158 1..1604 1604bp
FT                   BglII = A^GATCT
FT                   BclI =  T^GATCA"
FT   -               1605..3898
FT                   /note="pJRD158 1610..3903 2294bp"
FT   rep_origin      0..0
FT                   /note="ORI Gram negative bacteria"
XX
SQ   Sequence 3898 BP; 897 A; 1040 C; 954 G; 1007 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctac tgaagtggat ttctttaaga gctcctttaa cttcctcacc agtagttgta
     tcggtaccat aagtagaagc agcaacccaa gtagctttac cagcatccgg ttcaaccagc
     atagtaagaa tcttactgga catcggcagt tcttcgaaca gtgcgccaac taccagctct
     ttctgcagtt cattcagggc accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     gaaacgatcc tcatcctgtc tcttgatcgc attctaacgc gtttaatttt gatggggatt
     aaaacaaagg gggatttaac tggaggcgtg aaaaacgggg agctgatctt taaaagagga
     tatttgggag tacggtttag ctttcatacc accatctaga gtctttgttt tgacgccatt
     agcgtacgta acaatattat atttatctcc gacgatattc tcatcagtga aatccagctg
     gagttcttta gcaaattttt ttattagctg agatatagta tgatagtgat gaagttctag
     gtcttcacta ctaccacata tagcacagca accatcctta ctatacctag attttatacc
     atccctcatt atggaaatgg catcacgttt gtacttagta ttagtggcca tactcctcca
     atccaaagct atttagaaag attactatat cctcaaacag gcggtaaccg gcctcttcat
     cgggaatgcg cgcgaccttc agcatcgccg gcatgtcccc ctggcggacg ggaagtatcc
     agctcgaggt cgggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     tggtatctgc gctctgctga agccagttac cttcggaaaa agagttgata gctcttgatc
     cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     accgagttgc tcttgcccga cgtcaatacg ggataatacc gcgccacata gcagaacttt
     aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//