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ID   PJRD174    preliminary; circular DNA; SYN; 3864 BP.
XX
AC   IG9883;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Gram-negative bacteria plasmid vector pJRD174 - complete, phase.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJRD177 from pJRD173 & pJRD182
RC   pJRD178 from pJRD177 & pEMBL8
RC   pJRD179 from pJRD178 & pIC20R
RC   pJRD187 from pJRD179 & pCQV2
RC   pJRD187K from pJRD187 & pKMH4-10
RC   pJRD187Ka, pJRD187Kg from pJRD187 & pKMH4-10
RC   pJRD187Kc from pJRD187 & pKMH4-10
RC   pJRD215K from pJRD187K & pJRD215
RA   Davison J., Heusterspreute M., Chevalier N., Brunel F.;
RT   "A phase-shift fusion system for the regulation of foreign gene
RT   expression by lambda repressor in Gram-negative bacteria";
RL   Gene 60:227-235(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJRD174)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Gram-negative bacteria)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pJRD158)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pJRD158 BclI-BglII 3898bp 1610..3903..1605
FT                   -> pJRD173 3898bp [BclI-BglII]
FT                   1. pJRD173 remove BglII-XbaI 51bp, pJRD158 1610..1661
FT                   \ MCS/3852bp
FT                   2. linker BglII-XbaI 17bp gatcttaaggcctaggt
FT                   -> pJRD174 3869bp"
FT   -               1..1604
FT                   /note="pJRD158 1..1604 1604bp
FT                   BglII = A^GATCT
FT                   \         gatcttaaggcctaggt"
FT   -               1605..1621
FT                   /note="gatcttaaggcctaggt 17bp
FT                   \ gatcttaaggcctaggt
FT                   XbaI =            T^CTAGA"
FT   -               1622..3864
FT                   /note="pJRD158 1661..3903 2243bp"
FT   rep_origin      0..0
FT                   /note="ORI Gram negative bacteria"
XX
SQ   Sequence 3864 BP; 886 A; 1035 C; 948 G; 995 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctac tgaagtggat ttctttaaga gctcctttaa cttcctcacc agtagttgta
     tcggtaccat aagtagaagc agcaacccaa gtagctttac cagcatccgg ttcaaccagc
     atagtaagaa tcttactgga catcggcagt tcttcgaaca gtgcgccaac taccagctct
     ttctgcagtt cattcagggc accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     gaaacgatcc tcatcctgtc tcttgatcgc attctaacgc gtttaatttt gatggggatt
     aaaacaaagg gggatttaac tggaggcgtg aaaaacgggg agctgatctt aaggcctagg
     tctagagtct ttgttttgac gccattagcg tacgtaacaa tattatattt atctccgacg
     atattctcat cagtgaaatc cagctggagt tctttagcaa atttttttat tagctgagat
     atagtatgat agtgatgaag ttctaggtct tcactactac cacatatagc acagcaacca
     tccttactat acctagattt tataccatcc ctcattatgg aaatggcatc acgtttgtac
     ttagtattag tggccatact cctccaatcc aaagctattt agaaagatta ctatatcctc
     aaacaggcgg taaccggcct cttcatcggg aatgcgcgcg accttcagca tcgccggcat
     gtccccctgg cggacgggaa gtatccagct cgaggtcggg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttgatagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccgacgtc aatacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcgtcttca agaa
//