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ID   PJRD182    preliminary; circular DNA; SYN; 3783 BP.
XX
AC   IG9881;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Gram-negative bacteria plasmid vector pJRD182 - complete, phase.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJRD177 from pJRD173 & pJRD182
RC   pJRD178 from pJRD177 & pEMBL8
RC   pJRD179 from pJRD178 & pIC20R
RC   pJRD187 from pJRD179 & pCQV2
RC   pJRD187K from pJRD187 & pKMH4-10
RC   pJRD187Ka, pJRD187Kg from pJRD187 & pKMH4-10
RC   pJRD187Kc from pJRD187 & pKMH4-10
RC   pJRD215K from pJRD187K & pJRD215
RA   Davison J., Heusterspreute M., Chevalier N., Brunel F.;
RT   "A phase-shift fusion system for the regulation of foreign gene
RT   expression by lambda repressor in Gram-negative bacteria";
RL   Gene 60:227-235(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJRD182)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Gram-negative bacteria)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pJRD158)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pJRD158 RsaI-DdeI 3780bp 1896..3903..1773
FT                   -> pJRD182 3780bp"
FT   -               1..1775
FT                   /note="pJRD158 1..1775 1775bp
FT                   DdeI = C^TNA G
FT                   RsaI =    GT^AC"
FT   -               1776..3783
FT                   /note="pJRD158 1896..3903 2008bp"
FT   rep_origin      0..0
FT                   /note="ORI Gram negative bacteria"
XX
SQ   Sequence 3783 BP; 861 A; 1014 C; 938 G; 970 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctac tgaagtggat ttctttaaga gctcctttaa cttcctcacc agtagttgta
     tcggtaccat aagtagaagc agcaacccaa gtagctttac cagcatccgg ttcaaccagc
     atagtaagaa tcttactgga catcggcagt tcttcgaaca gtgcgccaac taccagctct
     ttctgcagtt cattcagggc accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     gaaacgatcc tcatcctgtc tcttgatcgc attctaacgc gtttaatttt gatggggatt
     aaaacaaagg gggatttaac tggaggcgtg aaaaacgggg agctgatcag atctttaaaa
     gaggatattt gggagtacgg tttagctttc ataccaccat ctagagtctt tgttttgacg
     ccattagcgt acgtaacaat attatattta tctccgacga tattctcatc agtgaaatcc
     agctggagtt ctttagcaaa tttttttatt agctggtact tagtattagt ggccatactc
     ctccaatcca aagctattta gaaagattac tatatcctca aacaggcggt aaccggcctc
     ttcatcggga atgcgcgcga ccttcagcat cgccggcatg tccccctggc ggacgggaag
     tatccagctc gaggtcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tgatagctct
     tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     cggcgaccga gttgctcttg cccgacgtca atacgggata ataccgcgcc acatagcaga
     actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     gaa
//