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ID   PJS132     preliminary; circular DNA; SYN; 3441 BP.
XX
AC   IG9830;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pJS132 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJS132 from pKK232-8 & pUC19
RC   pJS133 from pJS132 & pUC18
RA   Shiau A., Smith J.M.;
RT   "Improved cat gene cassette for promoter analysis and genetic
RT   constructions";
RL   Gene 67:295-299(1988).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJS132)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli DH5alpha)(E.coli)(E.coli DH1)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pKK232-8)(pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK232-8 AvaI-BanI/HgiCI 766bp 183..949,
FT                   \ cat gene
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pUC19 remove AvaI-HincII 19bp 413..432, MCS/2667bp
FT                   T4 DNA polymerase:
FT                   -> pJS132 3433bp [SmaI at each end of cat]"
FT   -               1..416
FT                   /note="pUC19 1..416 416bp
FT                   AvaI =  C^YCGR G
FT                   AvaI =       C^YCGRG"
FT   -               417..1186
FT                   /note="pKK232-8 183..952 770bp
FT                   HgiCI = BanI = G^GYRC C
FT                   HindII = HincII = GTY^RAC"
FT   -               1187..3441
FT                   /note="pUC19 432..2686 2255bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-
FT                   [CAT]-HindIII-PstI-SalI-BamHI-SmaI-KpnI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
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SQ   Sequence 3441 BP; 867 A; 841 C; 863 G; 870 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggccgg
     ggatccgtcg acctgcagcc aagcttgagt aggacaaatc cgccgagctt cgacgagatt
     ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     atcccaatcg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgcgacc tgcaggcatg
     caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     gtatcacgag gccctttcgt c
//