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ID   PJS133     preliminary; circular DNA; SYN; 3456 BP.
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AC   ATCC37617;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pJS133 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJS132 from pKK232-8 & pUC19
RC   pJS133 from pJS132 & pUC18
RA   Shiau A., Smith J.M.;
RT   "Improved cat gene cassette for promoter analysis and genetic
RT   constructions";
RL   Gene 67:295-299(1988).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Plasmid with a promoterless CAT (chloramphenicol acetyltransferase)
CC   cassette that can be excised for use in genetic constructions.  CAT
CC   expression reflects transcriptional activity only;translation stops in
CC   all 3 reading frames.
CC   There is an EcoRI site within the CAT gene.
CC   Restriction digests of the clone give the following sizes (kb):
CC   XbaI--3.5; HindIII--2.75, 0.75. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pJS133)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DH5alpha)(E.coli)(E.coli DH1)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pKK232-8)(pUC19)(pUC18)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK232-8 AvaI-BanI/HgiCI 766bp 183..949,
FT                   \ cat gene
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pUC19 remove AvaI-HincII 19bp 413..432, MCS/2667bp
FT                   T4 DNA polymerase:
FT                   -> pJS132 3433bp [SmaI at each end of cat]
FT                   1. pJS132 SmaI/XmaI-SmaI/XmaI 764bp 185..949, Tn9 cat
FT                   \ pUC19 415/pKK232-8 185/#V00622
FT                   2. pUC18 SmaI/XmaI 2686bp 437..437
FT                   -> pJS133 3450bp"
FT   -               1..436
FT                   /note="pUC18 1..436 436bp
FT                   SmaI = XmaI = CCC^GG G"
FT   -               437..438
FT                   /note="pUC19 413..414 [gg] 2bp
FT                   SmaI = XmaI = CCC^GG G
FT                   AvaI =        C^YCGR G
FT                   HgiCI = BanI =     G^GYRCC"
FT   -               439..1206
FT                   /note="pKK232-8 185..952 768bp complement
FT                   AvaI =        C^YCGR G
FT                   SmaI = XmaI = CCC^GG G"
FT   -               1207..3456
FT                   /note="pUC18 437..2686 2250bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-
FT                   [CAT]-HindIII-PstI-SalI-BamHI-SmaI-KpnI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
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SQ   Sequence 3456 BP; 883 A; 858 C; 853 G; 862 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
     actctagagg atccccgggc accaataact gccttaaaaa aattacgccc cgccctgcca
     ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
     ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgcgattg
     ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     tgaaaatctc gtcgaagctc ggcggatttg tcctactcaa gcttggctgc aggtcgacgg
     atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     ttctcccttc gggaagcgtg gcgctttctc aaagctcacg ctgtaggtat ctcagttcgg
     tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     cctataaaaa taggcgtatc acgaggccct ttcgtc
//