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ID   PJSC73     preliminary; circular DNA; SYN; 3564 BP.
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AC   K01092; L08920; VB0008; ATCC37262;
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DT   01-AUG-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pJSC73 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3564
RC   pJSC7 from pAT153 & pBR325
RC   pJSC73 from pJSC7
RA   Cordingley J.S., Taylor D.W., Dunne D.W., Butterworth A.E.;
RT   "Clone banks of cDNA from the parasite Schistosoma mansoni:
RT   isolation of clones containing a potentially immunodiagnostic
RT   antigen gene";
RL   Gene 26:25-39(1983).
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RN   [2]
RP   1-3564
RC   pJSC73
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
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CC   This vector was used to construct cDNA clone
CC   banks from mRNA from the human parasite Schistosoma mansoni and
CC   several other sources [1].
CC   Small plasmid vector for cloning of blunt-end DNA fragments (HincII in
CC   ampR and PvuII in cmlR).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pJSC73)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)(Schistosoma mansoni)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR325)(pAT153)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAT153 HindIII-PstI 2879bp 30..2909
FT                   2. pBR325 PstI-HindIII 2412bp 3612..5994..30
FT                   -> pJSC7
FT                   1. pJSC7 BamHI-SalI
FT                   S1 nuclease
FT                   -> pJSC73 3564bp [no HincII in tet]"
FT   CDS             3451..3564
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   CDS             1..546
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   misc_feature    0..0
FT                   /note="immunodiagnostic antigen"
FT   misc_feature    0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique HincII"
FT   CDS             complement(2183..2971)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    1..803
FT                   /note="from pBR325"
FT   misc_feature    complement(814..901)
FT                   /note="from pBR325"
FT   misc_feature    902..1238
FT                   /note="from pBR325"
FT   misc_feature    1235..3564
FT                   /note="from pBR325"
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SQ   Sequence 3564 BP; 906 A; 874 C; 868 G; 916 T; 0 other;
     ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa
     gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc
     agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt tattgactac
     cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc
     cgtggaggta ataattgacg atagatcatt tattctgcct ccccgcgttg cgtcgcggtg
     catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
     cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
     aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
     aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
     ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg
     actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     ttcgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct
     gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta
     agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     tgtacctata accagaccgt tcag
//