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ID   PJY1       preliminary; circular DNA; SYN; 9604 BP.
XX
AC   IG9900;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mammal/E. coli phagemid vector pJY1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pSKS105 & linker
RC   pFRTbetaGAL from plasmid & pSV2
RC   plasmid2 from pUC19 & FRT gene & pMC1neo-polyA
RC   pNEObetaGAL from pFRTbetaGAL & plasmid2
RC   pOG28 from pCDM8 & mouse DHFR gene
RC   pOG44 from pCDM8 & pMLSIScat & yeast FLP recombinase & yeast 2 micron
RC   pOG45 from pNEObetaGAL & pUC19
RC   pOG45A from pOG45
RA   O'Gorman S., Fox D.T., Wahl G.W.;
RT   "Recombinase-mediated gene activation and site-specific integration
RT   in mammalian cells";
RL   Science 251:1351-1355(1991).
XX
RN   [2]
RC   pMLSIScat from cat gene
RA   Huang M.T., Gorman C.M.;
RT   "Intervening sequences increase efficiency of RNA 3' processing
RT   and accumulation of cytoplasmic RNA";
RL   Nucleic Acids Res. 18:937-947(1990).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJY1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(mammalian tissue culture cells)
CC   CP ()
CC   FN (gene targeting)
CC   SE ()
CC   PA (pBR322)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. SV40 BamHI 5243bp 2534..2534
FT                   -> pJY1 9604bp"
FT   -               1..375
FT                   /note="pBR322 1..375 375bp
FT                   BamHI = G^GATCC"
FT   -               376..5618
FT                   /note="SV40 2534..5243..2533 5243bp
FT                   BamHI = G^GATCC"
FT   -               5619..9604
FT                   /note="pBR322 376..4361 3986bp"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 late gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 9604 BP; 2501 A; 2310 C; 2173 G; 2620 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     atggctgatt atgatcatga acagactgtg aggactgagg ggcctgaaat gagccttggg
     actgtgaatc aatgcctgtt tcatgccctg agtcttccat gttcttctcc ccaccatctt
     catttttatc agcattttcc tggctgtctt catcatcatc atcactgttt cttagccaat
     ctaaaactcc aattcccata gccacattaa acttcatttt ttgatacact gacaaactaa
     actctttgtc caatctctct ttccactcca caattctgct ctgaatactt tgagcaaact
     cagccacagg tctgtaccaa attaacataa gaagcaaagc aatgccactt tgaattattc
     tcttttctaa caaaaactca ctgcgttcca ggcaatgctt taaataatct ttgggcctaa
     aatctatttg ttttacaaat ctggcctgca gtgttttagg cacactgtac tcattcatgg
     tgactattcc agggggaaat atttgagttc ttttatttag gtgtttcttt tctaagttta
     ccttaacact gccatccaaa taatccctta aattgtccag gttattaatt ccctgacctg
     aaggcaaatc tctggactcc cctccagtgc cctttacatc ctcaaaaact actaaaaact
     ggtcaatagc tactcctagc tcaaagttca gcctgtccaa gggcaaatta acatttaaag
     ctttcccccc acataattca agcaaagcag ctgctaatgt agttttacca ctatcaattg
     gtcctttaaa cagccagtat ctttttttag gaatgttgta caccatgcat tttaaaaagt
     catacaccac tgaatccatt ttgggcaaca aacagtgtag ccaagcaact ccagccatcc
     attcttctat gtcagcagag cctgtagaac caaacattat atccatccta tccaaaagat
     cattaaatct gtttgttaac atttgttctc tagttaattg taggctatca acccgctttt
     tagctaaaac agtatcaaca gcctgttggc atatggtttt ttggtttttg ctgtcagcaa
     atatagcagc atttgcataa tgcttttcat ggtacttata gtggctgggc tgttcttttt
     taatacattt taaacacatt tcaaaactgt actgaaattc caagtacatc ccaagcaata
     acaacacatc atcacatttt gtttccattg catactctgt tacaagcttc caggacactt
     gtttagtttc ctctgcttct tctggattaa aatcatgctc ctttaaccca cctggcaaac
     tttcctcaat aacagaaaat ggatctctag tcaaggcact atacatcaaa tattccttat
     taaccccttt acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag
     cagacactct atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat
     gcctacttat aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt
     tttcctttgt ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac
     tcaaaaaact tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt
     ttggaggagt agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt
     ctgagcaaaa caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc
     cataggttgg aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac
     ttaaaaattt tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg
     tcacaccaca gaagtaaggt tccttcacaa agatcaagtc caaaccacat tctaaagcaa
     tcgaagcagt agcaatcaac ccacacaagt ggatctttcc tgtataattt tctattttca
     tgcttcatcc tcagtaagca cagcaagcat atgcagttag cagacatttt ctttgcacac
     tcaggccatt gtttgcagta cattgcatca acaccaggat ttaaggaaga agcaaatacc
     tcagttgcat cccagaagcc tccaaagtca ggttgatgag catattttac tccatcttcc
     attttcttgt acagagtatt cattttcttc attttttctt catctcctcc tttatcagga
     tgaaactcct tgcatttttt taaatatgcc tttctcatca gaggaatatt cccccaggca
     ctcctttcaa gacctagaag gtccattagc tgcaaagatt cctctctgtt taaaacttta
     tccatctttg caaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     gttctttccg cctcagaagg tacctaacca agttcctctt tcagaggtta tttcaggcca
     tggtgctgcg ccggctgtca cgccaggcct ccgttaaggt tcgtaggtca tggactgaaa
     gtaaaaaaac agctcaacgc ctttttgtgt ttgttttaga gcttttgctg caattttgtg
     aaggggaaga tactgttgac gggaaacgca aaaaaccaga aaggttaact gaaaaaccag
     aaagttaact ggtaagttta gtctttttgt cttttatttc aggtccatgg gtgctgcttt
     aacactgttg ggggacctaa ttgctactgt gtctgaagct gctgctgcta ctggattttc
     agtagctgaa attgctgctg gagaggccgc tgctgcaatt gaagtgcaac ttgcatctgt
     tgctactgtt gaaggcctaa caacctctga ggcaattgct gctataggcc tcactccaca
     ggcctatgct gtgatatctg gggctcctgc tgctatagct ggatttgcag ctttactgca
     aactgtgact ggtgtgagcg ctgttgctca agtggggtat agatttttta gtgactggga
     tcacaaagtt tctactgttg gtttatatca acaaccagga atggctgtag atttgtatag
     gccagatgat tactatgata ttttatttcc tggagtacaa acctttgttc acagtgttca
     gtatcttgac cccagacatt ggggtccaac actttttaat gccatttctc aagctttttg
     gcgtgtaata caaaatgaca ttcctaggct cacctcacag gagcttgaaa gaagaaccca
     aagatattta agggacagtt tggcaaggtt tttagaggaa actacttgga cagtaattaa
     tgctcctgtt aattggtata actctttaca agattactac tctactttgt ctcccattag
     gcctacaatg gtgagacaag tagccaacag ggaagggttg caaatatcat ttgggcacac
     ctatgataat attgatgaag cagacagtat tcagcaagta actgagaggt gggaagctca
     aagccaaagt cctaatgtgc agtcaggtga atttattgaa aaatttgagg ctcctggtgg
     tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggaag
     tgttacttct gctctaaaag cttatgaaga tggccccaac aaaaagaaaa ggaagttgtc
     caggggcagc tcccaaaaaa ccaaaggaac cagtgcaagt gccaaagctc gtcataaaag
     gaggaataga agttctagga gttaaaactg gagtagacag cttcactgag gtggagtgct
     ttttaaatcc tcaaatgggc aatcctgatg aacatcaaaa aggcttaagt aaaagcttag
     cagctgaaaa acagtttaca gatgactctc cagacaaaga acaactgcct tgctacagtg
     tggctagaat tcctttgcct aatttaaatg aggacttaac ctgtggaaat attttgatgt
     gggaagctgt tactgttaaa actgaggtta ttggggtaac tgctatgtta aacttgcatt
     cagggacaca aaaaactcat gaaaatggtg ctggaaaacc cattcaaggg tcaaattttc
     atttttttgc tgttggtggg gaacctttgg agctgcaggg tgtgttagca aactacagga
     ccaaatatcc tgctcaaact gtaaccccaa aaaatgctac agttgacagt cagcagatga
     acactgacca caaggctgtt ttggataagg ataatgctta tccagtggag tgctgggttc
     ctgatccaag taaaaatgaa aacactagat attttggaac ctacacaggt ggggaaaatg
     tgcctcctgt tttgcacatt actaacacag caaccacagt gcttcttgat gagcagggtg
     ttgggccctt gtgcaaagct gacagcttgt atgtttctgc tgttgacatt tgtgggctgt
     ttaccaacac ttctggaaca cagcagtgga agggacttcc cagatatttt aaaattaccc
     ttagaaagcg gtctgtgaaa aacccctacc caatttcctt tttgttaagt gacctaatta
     acaggaggac acagagggtg gatgggcagc ctatgattgg aatgtcctct caagtagagg
     aggttagggt ttatgaggac acagaggagc ttcctgggga tcctctacgc cggacgcatc
     gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     agcctaactt cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc
     acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg
     ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg
     ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg
     cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc
     ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
     ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
     gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     agaa
//