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ID   PJYM       preliminary; circular DNA; SYN; 8210 BP.
XX
AC   IG9901;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Mammal/E. coli phagemid vector pJYM - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pSKS105 & linker
RC   pFRTbetaGAL from plasmid & pSV2
RC   plasmid2 from pUC19 & FRT gene & pMC1neo-polyA
RC   pNEObetaGAL from pFRTbetaGAL & plasmid2
RC   pOG28 from pCDM8 & mouse DHFR gene
RC   pOG44 from pCDM8 & pMLSIScat & yeast FLP recombinase & yeast 2 micron
RC   pOG45 from pNEObetaGAL & pUC19
RC   pOG45A from pOG45
RA   O'Gorman S., Fox D.T., Wahl G.W.;
RT   "Recombinase-mediated gene activation and site-specific integration
RT   in mammalian cells";
RL   Science 251:1351-1355(1991).
XX
RN   [2]
RC   pMLSIScat from cat gene
RA   Huang M.T., Gorman C.M.;
RT   "Intervening sequences increase efficiency of RNA 3' processing
RT   and accumulation of cytoplasmic RNA";
RL   Nucleic Acids Res. 18:937-947(1990).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pJYM)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(mammalian tissue culture cells)
CC   CP ()
CC   FN (gene targeting)
CC   SE ()
CC   PA (pBR322)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. SV40 BamHI 5243bp 2534..2534
FT                   -> pJY1 9604bp
FT                   1. pJY1 remove 1393bp, pBR322 1092..2485 poison seq
FT                   \ 8211bp
FT                   -> pJYM 8211bp"
FT   -               1..375
FT                   /note="pBR322 1..375 375bp
FT                   BamHI = G^GATCC"
FT   -               376..5618
FT                   /note="SV40 2534..5243..2533 5243bp
FT                   BamHI = G^GATCC"
FT   -               5619..6334
FT                   /note="pBR322 376..1091 716bp"
FT   -               6335..8210
FT                   /note="pBR322 2486..4361 1876bp"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 late gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 8210 BP; 2185 A; 1907 C; 1792 G; 2326 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     atggctgatt atgatcatga acagactgtg aggactgagg ggcctgaaat gagccttggg
     actgtgaatc aatgcctgtt tcatgccctg agtcttccat gttcttctcc ccaccatctt
     catttttatc agcattttcc tggctgtctt catcatcatc atcactgttt cttagccaat
     ctaaaactcc aattcccata gccacattaa acttcatttt ttgatacact gacaaactaa
     actctttgtc caatctctct ttccactcca caattctgct ctgaatactt tgagcaaact
     cagccacagg tctgtaccaa attaacataa gaagcaaagc aatgccactt tgaattattc
     tcttttctaa caaaaactca ctgcgttcca ggcaatgctt taaataatct ttgggcctaa
     aatctatttg ttttacaaat ctggcctgca gtgttttagg cacactgtac tcattcatgg
     tgactattcc agggggaaat atttgagttc ttttatttag gtgtttcttt tctaagttta
     ccttaacact gccatccaaa taatccctta aattgtccag gttattaatt ccctgacctg
     aaggcaaatc tctggactcc cctccagtgc cctttacatc ctcaaaaact actaaaaact
     ggtcaatagc tactcctagc tcaaagttca gcctgtccaa gggcaaatta acatttaaag
     ctttcccccc acataattca agcaaagcag ctgctaatgt agttttacca ctatcaattg
     gtcctttaaa cagccagtat ctttttttag gaatgttgta caccatgcat tttaaaaagt
     catacaccac tgaatccatt ttgggcaaca aacagtgtag ccaagcaact ccagccatcc
     attcttctat gtcagcagag cctgtagaac caaacattat atccatccta tccaaaagat
     cattaaatct gtttgttaac atttgttctc tagttaattg taggctatca acccgctttt
     tagctaaaac agtatcaaca gcctgttggc atatggtttt ttggtttttg ctgtcagcaa
     atatagcagc atttgcataa tgcttttcat ggtacttata gtggctgggc tgttcttttt
     taatacattt taaacacatt tcaaaactgt actgaaattc caagtacatc ccaagcaata
     acaacacatc atcacatttt gtttccattg catactctgt tacaagcttc caggacactt
     gtttagtttc ctctgcttct tctggattaa aatcatgctc ctttaaccca cctggcaaac
     tttcctcaat aacagaaaat ggatctctag tcaaggcact atacatcaaa tattccttat
     taaccccttt acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag
     cagacactct atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat
     gcctacttat aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt
     tttcctttgt ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac
     tcaaaaaact tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt
     ttggaggagt agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt
     ctgagcaaaa caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc
     cataggttgg aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac
     ttaaaaattt tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg
     tcacaccaca gaagtaaggt tccttcacaa agatcaagtc caaaccacat tctaaagcaa
     tcgaagcagt agcaatcaac ccacacaagt ggatctttcc tgtataattt tctattttca
     tgcttcatcc tcagtaagca cagcaagcat atgcagttag cagacatttt ctttgcacac
     tcaggccatt gtttgcagta cattgcatca acaccaggat ttaaggaaga agcaaatacc
     tcagttgcat cccagaagcc tccaaagtca ggttgatgag catattttac tccatcttcc
     attttcttgt acagagtatt cattttcttc attttttctt catctcctcc tttatcagga
     tgaaactcct tgcatttttt taaatatgcc tttctcatca gaggaatatt cccccaggca
     ctcctttcaa gacctagaag gtccattagc tgcaaagatt cctctctgtt taaaacttta
     tccatctttg caaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     gttctttccg cctcagaagg tacctaacca agttcctctt tcagaggtta tttcaggcca
     tggtgctgcg ccggctgtca cgccaggcct ccgttaaggt tcgtaggtca tggactgaaa
     gtaaaaaaac agctcaacgc ctttttgtgt ttgttttaga gcttttgctg caattttgtg
     aaggggaaga tactgttgac gggaaacgca aaaaaccaga aaggttaact gaaaaaccag
     aaagttaact ggtaagttta gtctttttgt cttttatttc aggtccatgg gtgctgcttt
     aacactgttg ggggacctaa ttgctactgt gtctgaagct gctgctgcta ctggattttc
     agtagctgaa attgctgctg gagaggccgc tgctgcaatt gaagtgcaac ttgcatctgt
     tgctactgtt gaaggcctaa caacctctga ggcaattgct gctataggcc tcactccaca
     ggcctatgct gtgatatctg gggctcctgc tgctatagct ggatttgcag ctttactgca
     aactgtgact ggtgtgagcg ctgttgctca agtggggtat agatttttta gtgactggga
     tcacaaagtt tctactgttg gtttatatca acaaccagga atggctgtag atttgtatag
     gccagatgat tactatgata ttttatttcc tggagtacaa acctttgttc acagtgttca
     gtatcttgac cccagacatt ggggtccaac actttttaat gccatttctc aagctttttg
     gcgtgtaata caaaatgaca ttcctaggct cacctcacag gagcttgaaa gaagaaccca
     aagatattta agggacagtt tggcaaggtt tttagaggaa actacttgga cagtaattaa
     tgctcctgtt aattggtata actctttaca agattactac tctactttgt ctcccattag
     gcctacaatg gtgagacaag tagccaacag ggaagggttg caaatatcat ttgggcacac
     ctatgataat attgatgaag cagacagtat tcagcaagta actgagaggt gggaagctca
     aagccaaagt cctaatgtgc agtcaggtga atttattgaa aaatttgagg ctcctggtgg
     tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc tgtacggaag
     tgttacttct gctctaaaag cttatgaaga tggccccaac aaaaagaaaa ggaagttgtc
     caggggcagc tcccaaaaaa ccaaaggaac cagtgcaagt gccaaagctc gtcataaaag
     gaggaataga agttctagga gttaaaactg gagtagacag cttcactgag gtggagtgct
     ttttaaatcc tcaaatgggc aatcctgatg aacatcaaaa aggcttaagt aaaagcttag
     cagctgaaaa acagtttaca gatgactctc cagacaaaga acaactgcct tgctacagtg
     tggctagaat tcctttgcct aatttaaatg aggacttaac ctgtggaaat attttgatgt
     gggaagctgt tactgttaaa actgaggtta ttggggtaac tgctatgtta aacttgcatt
     cagggacaca aaaaactcat gaaaatggtg ctggaaaacc cattcaaggg tcaaattttc
     atttttttgc tgttggtggg gaacctttgg agctgcaggg tgtgttagca aactacagga
     ccaaatatcc tgctcaaact gtaaccccaa aaaatgctac agttgacagt cagcagatga
     acactgacca caaggctgtt ttggataagg ataatgctta tccagtggag tgctgggttc
     ctgatccaag taaaaatgaa aacactagat attttggaac ctacacaggt ggggaaaatg
     tgcctcctgt tttgcacatt actaacacag caaccacagt gcttcttgat gagcagggtg
     ttgggccctt gtgcaaagct gacagcttgt atgtttctgc tgttgacatt tgtgggctgt
     ttaccaacac ttctggaaca cagcagtgga agggacttcc cagatatttt aaaattaccc
     ttagaaagcg gtctgtgaaa aacccctacc caatttcctt tttgttaagt gacctaatta
     acaggaggac acagagggtg gatgggcagc ctatgattgg aatgtcctct caagtagagg
     aggttagggt ttatgaggac acagaggagc ttcctgggga tcctctacgc cggacgcatc
     gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     tccaggcagg tagatgacga ccatcaggga cagcaggcca gcaaaaggcc aggaaccgta
     aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//