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ID   PK184      preliminary; circular DNA; PRO; 2432 BP.
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AC   U00800; ATCC37766;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pK184 - complete.
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KW   cloning vector.
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OS   Escherichia coli
OC   Procaryotae; Gracilicutes; Scotobacteria;
OC   Facultatively anaerobic rods; Enterobacteriaceae; Escherichia.
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RN   [1]
RC   pK184 from pK18 & pACYC184
RC   pK194 from pK19 & pACYC184
RA   Jobling M.G., Holmes R.K.;
RT   "Construction of vectors with the p15a replicon, kanamycin
RT   resistance, inducible lacZ alpha and pUC18 or pUC19 multiple
RT   cloning sites";
RL   Nucleic Acids Res. 18:5315-5315(1990).
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RN   [2]
RC   pK184
RA   Jobling M.G.;
RT   ;
RL   Submitted (16-AUG-1993) by:
RL   Jobling M. G., Uniformed Services University of the Health Sciences,
RL   Microbiology, 4301 Jones Bridge Road, Bethesda, MD, 20814-4799 USA.
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CC   A general purpose plasmid vector for use in complementation analysis
CC   with vectors having a ColE1 or pMB1 origin of replication.
CC   pK184 consists of bases 2092-2661/0-1177 of pK18 and bases 1520-836 of
CC   pACYC184.   There are 2 XbaI sites in the plasmid.
CC   pK184 (ATCC 37766) and pK194 (ATCC 37767) have the multiple cloning
CC   sites in opposite orientations, interrupting the lacZalpha coding
CC   sequence.
CC   Constructed by M. G. Jobling. (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--2.4; BamHI--2.4; EcoRI--2.4; XbaI--1.45, 0.95. (ATCC staff)
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pK184)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli XL1-Blue)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pK18 remove BstBI/AsuII-NspI/NspHI 916bp
FT                   \ 1176..2092, pMB1 ori to kan gene/1745bp
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   2. pACYC184 SacII-ClaI 684bp 836..1520, p15A ori
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> pK184 2432bp"
FT   -10_signal      2160..2165
FT                   /note="S10 E. coli lac operon promoter"
FT   -35_signal      2126..2131
FT                   /note="S35 E. coli lac operon promoter"
FT   rep_origin      1861..1863
FT                   /note="ORI E. coli miniplasmid p15A
FT                   origin of vegetative DNA replication oriV"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             365..1159
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan)"
FT   CDS             join(2200..2432,1..52)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide (lacZalpha)"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SstI-KpnI-AvaI-SmaI-BamHI-XbaI-
FT                   SalI-AccI-HincII-SfeI-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SacI-KpnI-SmaI-XmaI-BamHI-
FT                   AccI-SalI-HincII-PstI-SphI-HindIII"
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SQ   Sequence 2432 BP; 551 A; 630 C; 711 G; 540 T; 0 other;
     cgataagcta gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
     gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt
     tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     actccaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc
     cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     tcttgacgag ttcttctgag cgggactctg gggttcgcga tgataagctg tcaaacatga
     gaattacaac ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg
     cactgaaatc tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg
     tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa
     ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc
     aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa
     atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa
     gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
     ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc
     ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     gagccgccag gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
     ccttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcg
     agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc
     gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     caacagttgc gcagcctgaa tggcgaatgg cg
//