back Return to this vector's summary.
ID   PKA1       preliminary; circular DNA; SYN; 3639 BP.
XX
AC   D13749;
XX
DT   12-DEC-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKA1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3639
RC   pKA1 from pBR322
RA   Kato S., Sekine S., Kim N., Umezawa Y., Abe N., Aoki T., Oh S.;
RT   "Construction of an intact-protein-encoding cDNA library using a
RT   multi-functional cloning vector, pKA1";
RL   Unpublished (1992).
XX
RN   [2]
RC   pKA1
RA   Kato S.;
RT   ;
RL   Submitted (24-NOV-1992) to DDBJ by:
RL   Kato S., Genetic Engineering Section, Sagami Chemical Research Center,
RL   4-4-1 Nishi-Ohnuma, Sagamihara, Kanagawa 229, Japan.
XX
CC   NM (pKA1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori
FT                   -> pKA1 3639bp"
FT   misc_feature    1..5
FT                   /note="SIT unique HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3639 BP; 947 A; 886 C; 908 G; 898 T; 0 other;
     agcttggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga
     actgaaaaac cagaaagtta actggtaagt ttagtctttt tgtcttttat ttcaggtccc
     ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc tttacttcta
     ggcctgtacg gaagtgttac ttctgctcta aaagctgctc gagtgtaaaa cgacggccag
     tacgtattta atacgactca ctatagggaa ttccaggggg gtggtggata tcctggtacc
     gcggccgcgg atctcccttt agtgagggtt aattggatcc agacatgata agatacattg
     atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     aaaacctcta caaatgtggt atggctgatt atgatccggc tgcctcgcgc gtttcggtga
     tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg
     ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     ctacagggcg cgtcccattc gccattcaca caggaaacag ctatgaccat gatcctctag
     agtcgacctg caggcatgca agctaggcct acatggcca
//