back Return to this vector's summary.
ID   PKC16      preliminary; circular DNA; SYN; 14457 BP.
XX
AC   ATCC37085;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKC16 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKC7 from pBR322 & Tn5, kan gene
RC   pKC10 from pBR322 & lambda, NcII segment
RC   pKC14 from pBR322 & lambda, NOP segment
RC   pKC16 from pKC7 & lambda, NOP segment
RC   pKC25 from pKC7
RC   pSGR3 from pKC16 & xth gene
RA   Rao R.N., Rogers S.G.;
RT   "A thermoinducible lambda phage-ColE1 plasmid chimera for the
RT   overproduction of gene products from cloned DNA segments";
RL   Gene 3:247-263(1978).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Vector for overproduction of gene product. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKC16)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli N100)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pKC7)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKC7 remove small ?BglII-BamHI ?..1848,
FT                   \ 5835bp
FT                   2. lambda cI857 BamHI-BamHI 6900bp, N/O/P genes
FT                   -> pKC16 11400bp [unique BamHI]
FT                   1. pBR322 BamHI-EcoRI 3984bp 376..4360
FT                   2. lambda BamHI-EcoRI 10473bp 34500..44973,
FT                   \ N/cI/O/P genes
FT                   \ lambda EcoRI 21227 26105 31748 39169 44973
FT                   \ lambda BamHI 5506 22347 27973 34500 41733
FT                   -> pKC16 14457bp"
FT   -               1..3984
FT                   /note="pBR322 376..4359 3984bp
FT                   EcoRI = G^AATTC"
FT   -               3985..14457
FT                   /note="lambda 34504..44976 10473bp complement
FT                   BamHI = G^GATCC"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda pL"
XX
SQ   Sequence 14457 BP; 3463 A; 3665 C; 3369 G; 3960 T; 0 other;
     gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
     caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg
     cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
     gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
     gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag
     ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagaattca ggacagacag tggctacggc tcagtttggg
     ttgtgctgtt gctgggcggc gatgacgcct gtacgcattt ggtgatccgg ttctgcttcc
     ggtattcgct taattcagca caacggaaag agcactggct aaccaggctc gccgactctt
     cacgattatc gactcaatgc tcttacctgt tgtgcagata taaaaaatcc cgaaaccgtt
     atgcaggctc taactattac ctgcgaactg tttcgggatt gcattttgca gacctctctg
     cctgcgatgg ttggagttcc agacgatacg tcgaagtgac caactaggcg gaatcggtag
     taagcgccgc ctcttttcat ctcactacca caacgagcga attaacccat cgttgagtca
     aatttaccca attttattca ataagtcaat atcatgccgt taatatgttg ccatccgtgg
     caatcatgct gctaacgtgt gaccgcattc aaaatgttgt ctgcgattga ctcttctttg
     tggcattgca ccaccagagc gtcatacagc ggcttaacag tgcgtgacca ggtgggttgg
     gtaaggtttg ggattagcat cgtcacagcg cgatatgctg cgcttgctgg catccttgaa
     tagccgacgc ctttgcatct tccgcactct ttctcgacaa ctctccccca cagctctgtt
     ttggcaatat caaccgcacg gcctgtacca tggcaatctc tgcatcttgc ccccggcgtc
     gcggcactac ggcaataatc cgcataagcg aatgttgcga gcacttgcag tacctttgcc
     ttagtatttc cttcaagctt tgccacacca cggtatttcc ccgatacctt gtgtgcaaat
     tgcatcagat agttgatagc cttttgtttg tcgttctggc tgagttcgtg cttaccgcag
     aatgcagcca taccgaatcc ggcttgtgat tgcgccatcc ccatagcagc catcacatca
     gtaccggaaa gagagtcaga agccgtggcc cgtggtgagt cgctcatcat cgggcttttt
     ggcgaatgaa atttagctac gctttcgagt ctcatgcgcc ttctccctgt acctgaatca
     atgttaggtt tccgcagaac actgcgccgg tatcgatata catttggttg gcaaacttga
     gtggtttcac tgctggcgta tgaccaaaga tgaacgtgtc cgcgcctttg atttctttca
     cgatcccgtt ttgtgagttg ctgattcgtt cgcggttcca gattacctgc tgatgatcaa
     ctggctttcc aaactcgtat tcgtcaaagg gataatcggc gtggcagata acatattttt
     tatctttgct caccagttcg atgattaacg gaagttcatc tgctttatgg gcaagagctt
     tagccagaat ttctttgtcg taatcgagat taaagaacca gccaccgcca ttaagcagcc
     agtgattaac gtttccacgc tctgataagc catcaatcat catttgctca tggtttccac
     gtacagctct gaaccagggg aatgtgatta attccaggca ttcaacgttc tctgcaccac
     gatcaaccaa atcgcccacc gagataagca ggtctttttt gttgtcgaat ccaatcgtat
     ccagtttgtt catcaggttc gtgtagcatc cgtgcagatc gccaactacc caaatatttc
     ggtatttgct gccatcaatt ttttcgtaat agcgcatctc tttcactcca tccgcgatga
     accatgagaa cgtcgttgac gatggcgtgc attttcccgt ctttatcatc aacgtatttt
     ctgaccgtac cgcgactaca tttcagtctg cgtgctactt ctgtctgatt tccgtatgct
     tcaacgagca tgtctggaat ggtttttact gagaacgtca tgcggcctca cttctgctat
     ttcgcaggtc tttgagtttc tgttggtact ctgccttgat cgccttgcac tcttcgatag
     tccagcgatg gcggttatgg tttgattcga tttcgtctac tgcttcctgc ccgatgcggc
     taatcagttc gacgcgatac ggaacgagat ttccgctttt gtgctggttg cacaccacgc
     attgcttgtg aatattgcgt tcattaaatc ggagttgagg tgccgcagca gttgtccggt
     aatgtccggc atcccactga gcagacgtga gcgttccgca cgagatacat ggtaagtcgc
     ggtctctttc tctgatgaag gcgtttacgg cttgttgggc ttgtttaatc cagtaactgc
     ggggctttaa ggcgagtttt cgaatcttaa gtttatcttt ctgtttctgc tcctctcgtc
     gtcgtttctt ctctgctgct ttttccgctt tttcgcgttc tttacttcgt cgttcgagtg
     ctatcttggt tccacactct ggagagcacc accactgatt agcgaatgca gggtgaaacc
     attcccggca ttcatcgttt ttacatcgtc ttcgcgctgg tttagccatc atcttcttcc
     tcgtgcatcg agctattcgg atcgctcatc agttctgcgc agcagtgctc acacacgtga
     acttccagca catgcagctt ctgaccgcag ttagcgcacg ttaaagctcg ctcgacgctt
     tcttgttcgt aacttcgatt ttggtcaatc accttgtttt cctcgcacga cgtcttagcc
     accggatatc ccacaggtga gccgtgtagt tgaaggtttt tacgtcagat tcttttggga
     ttggcttggg tttatttctg gtgcgtttcg ttggaaggta tttgcagttt tcgcagatta
     tgtcggtgat acttcgtcgc tgtctcgcca cacgtcctcc ttttcctgcg gtagtggtaa
     cacccctgtt ggtgttcttt cacaccggag acaccatcga ttccagtaag gttgatttgg
     tcggaagcgg ttatcttctt tgcattcacc gcaccgataa catcgcatca tgcagcttcc
     ctcccgaagt cgaaatcaag ctgccctcca aatatttcgc atgactcaga acaagagccg
     gtatcgaatc ttttagctcg taccatgtcc tgatacaggg cttgataatc attttctgaa
     tacattttcg cgataccgtc cagcgacatt cttcctcggt acataatctc ctttggcgtt
     tcccgatgtc cgtcacgcac atgggatccc gtgatgacct cattaaaaac acgctgcaat
     ccctcctcat ctttgcaggc aagtccgatt ttttgcgttg attttttaat gcagaatatg
     cagttaccga gatgttccgg tatttgcaaa tcgaatggtt gttgcttcca ccatgcgagg
     atatcttcct tctcaaagtc tgacagttca gcaagatatc tgattccagg ctttggcttt
     agccgcttcg gttcatcagc tctgatgcca atccacgtgg tgtaattccc tcgcccgaaa
     tggtcatcac agtatttggt gaagggaacg agttttaatc tgtcagtgca gaacgcgccg
     ccgacgtatg gagtgccata tttctttacc atatcgataa atggcttcag aacaggcatt
     cgcgtctgaa tatcctttgg ttcccatacc gtataaccat ttggctgtcc aagctccggg
     ttgatatcaa cctgcaatac ggtgagcggt atatcccaga acttcacaac ttccctgaca
     aaccgatatg tcattggatg ttcacaacct gtatccatga aaacgtaatg cacgtcttta
     cctgcccgtc gcttttgctc cattagccag agcaaatatg ctgacgtcct gccaccggag
     aaactaacga catttatcat gcagccctgt ctccccatct cgctttccac tccagagcca
     gtctcgcttc gtctgaccac ttaacgccac gctctgtacc gaatgcctgt ataagctcta
     atagctccgc aaattcgcct acacgcatcc tgctggttga ctggcctatt accacaaagc
     cattcccggc aaggttagga acaacatcct gctgctttaa tgctgcggta aacacacact
     tccagctttc tgcatccagc cagcgaccat gccattcaac ctgacgagag acgtcaccta
     agcaggccca tagcttcctg ttttggtcta agctgcggtt gcgttcctga atggttacta
     cgattggttt ggttgggtct ggaaggattt gctgtactgc gtgaatagcg ttttgctgat
     gtgctggaga tcgaatttca aaggttagtt ttttcatgac ttccctctcc cccaaataaa
     aaggcctgcg attaccagca ggcctgttat tagctcagta atgtagatgg tcatctttta
     actccatata ccgccaatac ccgtttcatc gcggcactct ggcgacactc cttaaaaacc
     aggttcgtgc tcatctttcc ttcccgttct tccctggtag caaaccggta atacaccgtt
     cgccagacct taccttcgat aaccagaaga cctgcccgtg ccattttagc cgcggcctga
     tttatgctgg ttactgttgc gcctgttagc gcggcaacgt ccggcgcaca gaagctatta
     tgcgtcccca ggtaatgaat aattgcctct ttgcccgtca tacacttgct cctttcagtc
     cgaacttagc tttgatttct gcgatcttcg ccagagcctg tgcacgattt agaggtctac
     cgcccatgac aggaagttgt tttactggtt cagggatcgc ctcaccacgg ttaattctcg
     cagtcatatg gacaagctca tctgcggcct tacggcgtaa ttccgcatca gtaagcgcat
     tggcccgcat gttctgatac aggttggtaa ccagccagta gtgcgcgttt gatttccacg
     gataagactc cgcatccgga tacaggcctc gcttccggca atactcgtaa accatatcaa
     ccagctcgct gacgtttggc agtccggcgg taacggatgc ttcttcccgg caccatgcaa
     caaactgccc gggtgatggc agaaatggtc gattctgccg acgggctacg cgcattcctg
     cgttaacctg ttccatcgtg gtgatcccgt tttcccgaaa agccagaacc cactggcgac
     ggatttcgtt cacttcgttc tggtcacggt tagccaggct cgccgggaaa gttgccagta
     actggctgaa cacaccgttg atgatctgcg ctacctgctg tacctgcggc ttttcgtcgt
     actgttccgg catgttgttg gcgatccgac gcatctgctc acggtcaaag ttaaccatct
     gtgcggcgat gtttttcata gatccacccc gtaaatccag tctgtgtttg tcaggtcgag
     ttttggtttg ctggctgtca cgcctgcctg ttgcttgtta cggttgattt cgagttgggt
     ccacttatcg cggagtttgg ccgggctcag cacgttaccg gaccagaagt tgtcctggca
     tgcccagcgg aacagcacac acatgtcgcg gtggttacgt ccgtcacgtt cacgcatcag
     gcggatatcg ttagcccacc cagcaaaatt cggttttctg gctgatggtg cgatagtctt
     caccatgtca aacatccact ctgcggcggt caggtcttct gctgtccccc acttgctgcc
     gctctgaatt gcagcatccg gtttcaccac agaaaggtcg ttttctggct ggtcagagga
     ttcgccagaa ttctctgacg aataatcttt tcttttttct tttgtaatag tgtcttttgt
     gtccccctgt tttgagggat agcaatcccc caatttgagg gatgttttat ccctcgtttt
     aggggatttt ccctcgtttt gagggatgca ccattctgag atgtttttat ttggtccaaa
     catgccgcct tgctgcttga taatattcat tctgacgagt tctaacttgg cttcattgca
     ccgtttgaca ggtaactttg taatctcgct aagttgagaa tcggtgattc tgtccattgg
     tttattccac ccataggttt tacgcagaat ggcaagcagc actttaaact gtcgcttggt
     cagatctgcg cccgaataag cctcaagcag catatttgat agtctggcgt aaccatcatc
     gagatctgcc acattacgct cctgtccggc aaagttacct ctgccgaagt tgagtatttt
     tgctgtattt gtcataatga ctcctgttga tagatccagt aatgacctca gaactccatc
     tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatcgcagca
     acttgtcgcg ccaatcgagc catgtcgtcg tcaacgaccc cccattcaag aacagcaagc
     agcattgaga actttggaat ccagtccctc ttccacctgc tgatctgcga cttatcaacg
     cccacagctt ccgctgtctt ctcagttcca agcattgcga ttttgttaag caacgcactc
     tcgattcgta gagcctcgtt gcgtttgttt gcacgaacca tatgtaagta tttccttaga
     taacaattga ttgaatgtat gcaaataaat gcatacacca taggtgtggt ttaatttgat
     gccctttttc agggctggaa tgtgtaagag cggggttatt tatgctgttg tttttttgtt
     actcgggaag ggctttacct cttccgcata aacgcttcca tcagcgttta tagttaaaaa
     aatctttcgg cctgcatgaa tggccttgtt gatcgcgctt tgatatacgc cgagatcttt
     agctgtcttg gtttgcccaa agcgcattgc ataatctttc agggttatgc gttgttccat
     acaacctcct tagtacatgc aaccattatc accgccagag gtaaaatagt caacacgcac
     ggtgttagat atttatccct tgcggtgata gatttaacgt atgagcacaa aaaagaaacc
     attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa
     aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg
     cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc
     aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga
     gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt
     ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc
     ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga
     aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat
     tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg
     tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact
     aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat
     cgctagtcag tggcctgaag agacgtttgg ctgatcggca aggtgttctg gtcggcgcat
     agctgataac aattgagcaa gaatcttcat cgaattaggg gaattttcac tcccctcaga
     acataacata gtaaatggat tgaattatga agaatggttt ttatgcgact taccgcagca
     aaaataaagg gaaagataag cgctcaataa acctgtctgt tttccttaat tctctgctgg
     ctgataatca tcacctgcag gttggctcca attatttgta tattcataaa atcgatggaa
     aaacttttct ctttaccaaa acaaatgaca agagtctggt tcagaagata aatcgctcta
     aagcttcagt tgaagatatt aagaacagcc tcgcagatga cgaatcattg ggattcccat
     cttttttgtt tgttgaaggc gacaccattg gttttgccag aactgttttc gggccgacca
     catccgatct gacagatttt ttaatcggga aaggaatgtc attaagcagt ggagagcgcg
     ttcagataga gccactgatg aggggaacca ccaaagacga tgttatgcat atgcatttca
     tcggccgaac aacggtgaag gtagaagcca agctacctgt atttggcgat atattaaagg
     tcttaggggc aacagatatt gaaggggagc tttttgactc attggatata gtcattaagc
     caaaatttaa aagggatata aaaaaggttg ccaaggatat tatttttaac ccgtcacctc
     aattttcaga cattagcctg cgggcaaaag atgaggccgg agatatttta acagaacatt
     atctatcaga aaaaggccat ctctcagcgc ctctgaacaa ggtcaccaat gctgagatag
     ctgaagagat ggcatattgc tacgcaagaa tgaaaagtga tatactggaa tgttttaaaa
     ggcaggtggg caaagttaag gattaattat caggagtaat tatgcggaac agaatcatgc
     ctggtgttta catagtaata attccttacg ttatcgtaag catttgctat ctccttttcc
     gccactacat tcctggtgtt tctttttcag ctcatagaga tggtcttggg gcgacattgt
     catcatatgc aggaaccatg attgcaatcc tgattgctgc cttgacgttt ctaatcggaa
     gcagaacgcg ccgactggcc aagattagag agtatgggta tatgacatcg gtagttattg
     tctatgccct tagttttgtt gagcttggag ctttgttttt ctgcgggtta ttgcttcttt
     ccagcataag cggctacatg atacccacta tcgccatcgg cattgcctct gcatcgttca
     ttcatatatg catccttgtt ttccaactat ataatttgac cagagaacaa gaataacccg
     gcctcagcgc cgggttttct ttgcctcacg atcgccccca aaacacataa ccaattgtat
     ttattgaaaa ataaatagat acaactcact aaacatagca attcagatct ctcacctacc
     aaacaatgcc cccctgcaaa aaataaattc atataaaaaa catacagata accatctgcg
     gtgataaatt atctctggcg gtgttgacat aaataccact ggcggtgata ctgagcacat
     cagcaggacg cactgaccac catgaaggtg acgctcttaa aaattaagcc ctgaagaagg
     gcagcattca aagcagaagg ctttggggtg tgtgatacga aacgaagcat tggccgtaag
     tgcgattccg gattagctgc caatgtgcca atcgcggggg gttttcgttc aggactacaa
     ctgccacaca ccaccaaagc taactgacag gagaatccag atggatgcac aaacacgccg
     ccgcgaacgt cgcgcagaga aacaggctca atggaaagca gcaaatcccc tgttggttgg
     ggtaagcgca aaaccagtta accgccctat tctctcgctg aatcgcaaac cgaaatcacg
     agtagaaagc gcactaaatc cgatagacct tacagtgctg gctgaatacc acaaacagat
     tgaaagcaac ctgcaacgta ttgagcgcaa gaatcagcgc acatggtaca gcaagcctgg
     cgaacgcggc ataacatgca gtggacgcca gaaaattaag ggaaaatcga ttcctcttat
     ctagttactt agatattggc cttggcttta tctcaatatt atatggatca tagctggcaa
     ctaattcagt ccagtaaata tcctcaatag ggaataatat atgctttcca ttccatcggg
     aaaaagtttt gttcaacaca ccaagctcaa tcaactcact aatgtatggg aattgttttg
     atgtaaccac atacttcctg ccttcattaa gggctgcgca caaaaccata gattgctctt
     ctgtaaggtt ttgaattact gatcgcactt tatcgttttg catcttaatg cgttttctta
     gcttaaatcg cttatatctg gcgctggcaa tagctgataa tcgatgcaca ttaattgcta
     gcgaaaatgc aagagcaaag acgaaaacat gccacacatg aggaataccg attctctcat
     taacatattc aggccagtta tctgggctta aaagcagaag tccaacccag ataacgatca
     tatacatggt tctctccaga ggttcattac tgaacactcg tccgagaata acgagtg
//