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ID   PKC7       preliminary; circular DNA; SYN; 5835 BP.
XX
AC   IG0060; ATCC37084;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKC7 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pKC7 from pBR322 & Tn5, kan gene
RC   pKC10 from pBR322 & lambda, NcII segment
RC   pKC14 from pBR322 & lambda, NOP segment
RC   pKC16 from pKC7 & lambda, NOP segment
RC   pKC25 from pKC7
RC   pSGR3 from pKC16 & xth gene
RA   Rao R.N., Rogers S.G.;
RT   "A thermoinducible lambda phage-ColE1 plasmid chimera for the
RT   overproduction of gene products from cloned DNA segments";
RL   Gene 3:247-263(1978).
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CC   ATCC size is 5600 bp.
CC   Vector for overproduction of gene product. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKC7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli N100)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(Tn5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   transposition
FT                   2. Tn5 5818bp
FT                   -> pRZ102 10179bp
FT                   1. pRZ102 remove pBR322 sequences
FT                   -> pRZ112
FT                   1. pRZ112 HincII-HincII 2499bp 188..2687, neo gene
FT                   BamHI linker 10bp nnggatccnn:BamHI linker 10bp
FT                   \ nnggatccnn
FT                   HindIII-BamHI 1493bp 1196..2689, neo/kan gene
FT                   2. pBR322 remove HindIII-BamHI 346bp 30..376, 4015bp
FT                   -> plasmid 5509bp
FT                   1. plasmid HindIII 5509bp 30..30
FT                   2. lambda 326bp, pL
FT                   -> pKC7 5835bp [unique BglII in kan, BamHI]"
FT   CDS             411..1206
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo),
FT                   kanamycin resistance gene (kan),
FT                   in HindIII-BamHI fragment of Tn5, (replaces
FT                   HindIII-BamHI fragment of pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(4837..5697)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda pL"
XX
SQ   Sequence 5835 BP; 1233 A; 1658 C; 1617 G; 1327 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg gctcgatccc
     ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt ctaccggcag
     tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca tgcccccgaa
     ctgcaggagt ggggaggcac gatggccgct ttggtcgacc cggacgggac gctcctgcgc
     ctgatacaga acgaattgct tgcaggcatc tcatgagtgt gtcttcccgt tttccgcctg
     aggtcactgc gtggatggag cgctggcgcc tgctgcgcga cggcgagctg ctcaccaccc
     actcgagctg gatacttcgc gtccgccagg gggacatgcc ggcgatgctg aaggtcgccc
     gcattcccga tgaagaggcc ggttaccgcc tgttgacctg gtgggacggg cagggcgccc
     cccgactctt cgcctcggcg gcgggcgctc tgctcatgga gcgcgcgtcc ggggccgggg
     accttgcaca gatagcgtgg tccggccagg acgacgaggc ttgcagggat cctctacgcc
     ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc
     tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     gctcttacca gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc
     tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
     ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
     gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
     tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
     cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
     ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc
     gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat
     cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca
     gaaattcccc cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg cccttaacat
     ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
     ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
     ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
     ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     tttcgtcttc aagaa
//