back Return to this vector's summary.
ID   PKD1       preliminary; circular DNA; FUN; 4757 BP.
XX
AC   X03961; M24261;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Kluyveroymces plasmid vector pKD1 - complete.
XX
KW   cloning vector.
XX
OS   Kluyveromyces drosophilarum (yeast)
OC   Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
OC   Endomycetales; Saccharomycetaceae.
XX
RN   [1]
RP   1-4757
RC   pKD1 from Kluyveromyces
RC   B7, B11, P1, P3, E1, A10, A15, S11, HP1 from YIp5 & pKD1
RA   Chen X.J., Saliola M., Falcone C., Bianchi M.M., Fukuhara H.;
RT   "Sequence organization of the circular plasmid pKD1 from the yeast
RT   Kluyveromyces drosophilarum";
RL   Nucleic Acids Res. 14:4471-4481(1986).
XX
CC   This is the only circular plasmid known in the genus Kluyveromyces.
CC   The molecule exists in two isomeric forms generated by internal
CC   recombination at the two inverted repeats of 346 bp long.
CC   There is little homology to the 2micron plasmid of S.cerevisiae,
CC   except between the genes A and FLP.
CC   Plasmid can be amplified in E. coli RR1 when ligated to YIP5.
CC   NM (pKD1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Kluyveroymces)(K.drosophilarum)(K.lactis)(E.coli RRI)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YIp5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Kluyveroymces
FT                   -> pKD1 4757bp"
FT   rep_origin      0..0
FT                   /note="ORI Kluyveroymces 2 micron"
FT   CDS             0..0
FT                   /note="ANT Kluyveroymces URA3 gene (uracil)"
FT   repeat_unit     175..520
FT                   /note="RPT Kluyveroymces IR1 region"
FT   CDS             complement(462..1802)
FT                   /note="GEN Kluyveroymces Gene A"
FT   CDS             1986..2624
FT                   /note="GEN Kluyveroymces Gene C"
FT   repeat_unit     complement(2658..3003)
FT                   /note="RPT Kluyveroymces IR2 region"
FT   CDS             complement(3306..4550)
FT                   /note="GEN Kluyveroymces Gene B"
XX
SQ   Sequence 4757 BP; 1363 A; 997 C; 967 G; 1430 T; 0 other;
     ttctctcttg gggctgaagt gaaatttaaa aaagtcgctt gaggctcagc cggaattata
     aaacatcacc tgagtcttga gagcgctttc actcacctga ggctcagctg aaatttcaaa
     aagtcacttg agcccagaag gagtgtttca ccccctgagg ctataacgtt cgttatttta
     atacctaaat aaacaaaaat atatggtaca ggaacgcgag gcaacgcgcc gatacagggt
     caatgggtac acgagagggt gacactaggc gtagaaagtc attagtataa aatacagtgg
     tatatagtag atatttagtt tgttttcctt ttctttttct ccaaacgata tcagacattt
     gtctgataat gaagcattat cagacaaatg tctgatatcg tttttcaata ataatataca
     tcatcacaaa acaaacaaac atagcatcgc aagccccatc atgccaccac cgtccgctgt
     gatcgcaact catgtttccg gcggtattct gcaatgaatt ggagaacctc gtctgagata
     attccatgcc attgttcgaa caactggagg ctaggatgag ctgagaagga ttgagcgacc
     aagcgcggac ttgacggtgg gctgagtggt gggctaccaa ggctgttacc ctcctcttca
     agtagctcct cgcgagataa aggtttatta gaaggatcct tcaaaacata tatttcactg
     cccaatgggg cttccttgta aaaacctgat ataaaggcaa atacacggtc atctacagtc
     acactaccat gactgtagtg tgatctagcc actgcacttt gaatttcacg gtccccagcc
     caattgccca gtgaggagac atattttccc ttctcagatc tcgataaaaa ggtcgccatt
     aaatgtcttc caaaatgaga cttcgggccg ttccagatct tgaagactgg ttcatcgaca
     tgctgggtaa gaaacctaga gaacgttctg gccaatgact ctggtaaaaa ctgatgagtt
     tgattagttg gtctattact ggatactgtt ttttcaatag gcgagcaaac acgcaaatag
     tcgtataatg atatcagaag atcgcaatca ccattcacag gatagaagtt aacgtaccgt
     tcagttctgc ttttcgtttc tgtcacagta gcacgcacaa ttgggcccag aaatgaattg
     ttgtagatct caaaagtcct tggatctaga ttcttcagat cgctgtatct gcagcaattt
     ccaacagctc ccagaagtag caatcggtat tccgctcgtt ttgtagtggt tacgcaggac
     tgatcgaaga agcaggcaat cctggagaca atcttccaaa tatctttttc ttttgacaga
     atattagtga attgtaatcc aaccatagaa gcatcgtatt tatgtgtttc ctcgtagcga
     tcaaacaagg aaacttcttg atttttaaat gggctaacaa caaccttgta agaaggcaat
     gctgattcga tatccttttg cagagactct gtctttctta gtctaacagt gaatttgata
     attttgtcat ccttatcaaa agacagagat ttgccaattg cgctcttgta agagcggtag
     gtattgattt tcatctcgcg tcggatagat agcgactgca ttgtcaagat agagaatagg
     gacgccagct tatttctaat ttctttcgca tttatattaa aggtgtcaga ttccagaatt
     tcattaattt cattagcaca ctgatgaggt gtgaggtgag cagcctccgc aaaggtagac
     ataggggcat tggttggagg cctttgaggt accactagag tgctgcaaac atagcaccgt
     tcgagacttt aaaatcttca gttttaaaat tatgaaaaaa aacatcgtcc tgagttgaaa
     cggtcgtttc aacctccgtg tacagaaaga tacatagcat atggcaagct gcacgcagcg
     taaacatgcc ggacaactgt catttcgtca gatcagttga tctactctct gtgatactgc
     ttcgtttgtc cacggaggtc ggactaactc tcaccacgct tccacggcat tcgaaagaac
     taatattgta tcattgtaca tatgaggaac acgcagttga actgagcaaa ccaggactca
     ggaaagcagg aggtaagtgc tcgcttttcg tggatccaga ggaacgtgaa aattcgcctt
     ctcctcctat accgccgtat cagatatcag agatgcccct tcatgaactt ctcgagtcag
     gcaatgctaa attggttcca aatcccgagt ttgatctaac tgatccagac gactttcata
     agtgtttctc ggtcacctat tcagcattat ctttaatggt accatatctg cccagagctg
     ctctaaaggc tgctcgagtg ttttgtaaag atcattcaat attaacaacg gatatgcttg
     atttgaatta tcttgaagag ctaattgagt tctcaaagga aactgtgaac aaaatcccag
     ctagaatccc tatagaggac atgcttctcg agcggggata tgtgctacca tgggttcatg
     gtggtacagt gaagggagga aagctactga cccccaacga ttgattcttt accgaatcat
     tgcataattc attgcataat tcattgcaga ataccgccgg aaacatgagt tgcgatcaca
     gcggacggtg gtggcatgat ggggcttgcg atgctatgtt tgtttgtttt gtgatgatgt
     atattattat tgaaaaacga tatcagacat ttgtctgata atgcttcatt atcagacaaa
     tgtctgatat cgtttggaga aaaagaaaag gaaaacaaac taaatatcta ctatatacca
     ctgtatttta tactaatgac tttctacgcc tagtgtcacc ctctcgtgta cccattgacc
     ctgtatcggc gcgttgcctc gcgttcctgt accatatatt tttgtttatt taggtattaa
     aatttacttt cctcatacaa atattaaatt caccaaactt ctcaaaaact aattattcgt
     agttacaaac tctattttac aatcacgttt attcaaccat tctacatcca ataaccaaaa
     tgcccatgta cctctcagcg aagtccaacg gtactgtcca atattctcat taaatagtct
     ttcatctata tatcagaagg taattataat tagagatttc gaatcattac cgtgccgatt
     cgcacgctgc aacggcatgc atcactaatg aaaagcatac gacgcctgcg tctgacatgc
     actcattctg aagaagattc tgggcgcgtt tcgttctcgt tttcctctgt atattgtact
     ctggtggaca atttgaacat aacgtctttc acctcgccat tctcaataat gggttccaat
     tctatccagg tagcggttaa ttgacggtgc ttaagccgta tgctcactct aacgctaccg
     ttgtccaaac aacggacccc tttgtgacgg gtgtaagacc catcatgaag taaaacatct
     ctaacggtat ggaaaagagt ggtacggtca agtttcctgg cacgagtcaa ttttccctct
     tcgtgtagat cagaggctat atacatgccg aggtattcga tcactctacg atgacggtct
     gttagctcaa caacttcttc taaatgctcc ataaccgtaa cgtaagaagc ataactgtca
     atactgaagt catcccagtt tattggtgct cctgttgaac agtcatccac tatatgttcg
     aatagcccag gatcacgagg aggtcctaca aacggatacg gtacagtctt ctttttatag
     tctgcaaatt ctagaatagc attttttatc caatagtgtc gaatcgtcct agccgttcta
     ccgataaagg atccaatgtg attattagct ccactacacg atatgttaag tttgatcgat
     gtcttgttaa caaacgctaa actcaagttc ggcatttcca acagcgagaa gaaatcatca
     attccatcgg ctatctcttg ataagtcatt agatcatata ccttctcggg atgtcgttga
     gttactttat gactagaaat cttcaggtta tcatcaacgt aattgttctc caatagctct
     ggagagggac ataacaatac tttgattttt tccatggcct ggacttgttt ccgtaggaaa
     tacttgttct tttgtagacg ttccatgatg agtttgtata cctctgctgg agatatccat
     tctagatctt tgatataagt ttggtatggt aaagagttga ttttgtagga cacgtaaatc
     tgcgctagat aagtacattg tgcaaatgcc tctggtactt cgtaagaccc atgctgcgta
     attatagtat tattgagtgg atcataagcg ttgtactcgt ttttgaattt aaaactgtct
     aataaggccc tgtaaatctc tctgacttgt tgtacacctt tctgctcttc gggactgaga
     tcggatagca atggagcagc agtttctgag ctttctgatg gggctgacat ggcagatgcc
     tattcaatgc tgccttttgt ttgggaggtt atgaaatgca tctgtttaca ttgtatgtaa
     tacccttact aggcaatgtt ataagcaaaa atcctttgat cacatggaat atcactttat
     acgtgttgaa atatgcaaaa aaacagtccc cctgagctca gggggtggtt tacgcttttg
     aggctcagca gcgcgaa
//