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ID   PKH80      preliminary; circular DNA; SYN; 12637 BP.
XX
AC   ATCC37093;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/E.coli plasmid vector pKH80 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pEVP1, pEVP2 from pBD9 & pPLVP1
RC   pKH80 from pBD9 & pBR322
RC   pH98 from HBV core gene
RC   pKH81, pKH82, pKH83 from pKH80 & pH98, HBV core gene
RA   Hardy K., Stahl S., Kupper H.;
RT   "Production in B. subtilis of hepatitis B core antigen and a major
RT   antigen of foot and mouth disease virus";
RL   Nature 293:481-483(1981).
XX
RN   [2]
RC   [pFMDV88, pFMDV100 from FMDV]
RC   pFMDV-144, pFMDV-715 from pBR322 & FMDV VPg
RC   pFMDV-1034, pFMDV-1448, pFMDV-1824 from pBR322 & FMDV VPg
RC   pFMDV-217 from pBR322 & FMDV VPg
RC   pPLVP1 from pFMDV-1034 & pPLc24
RA   Kupper H., Keller W., Kurz C., Forss S., Schaller H., Franze R.,
RA   Strohmaier K., Marquardt O., Zaslavsky V.G., Hofschneider P.H.;
RT   "Cloning of cDNA of major antigen of foot and mouth disease virus
RT   and expression in E. coli";
RL   Nature 289:555-559(1981).
XX
RN   [3]
RC   pFMDV-1034 from FMDV
RA   Kurz C., Forss S., Kupper H., Strohmaier K., Schaller H.;
RT   "Nucleotide sequence and corresponding amino acid sequence of the
RT   gene for the major antigen of foot and mouth disease virus";
RL   Nucleic Acids Res. 9:1919-1931(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   An expression vector that can be propagated and selected for in either
CC   Bacillus subtilis or E.coli. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKH80)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Bacillus subtilis)(E.coli)
CC   HO (Bacillus subtilis BD170)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBD9)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBD9 EcoRI 8276bp 2..2, kan/ery/ori
FT                   \ pUB110 EcoRI 2..2 [8000bp]
FT                   2. pBR322 EcoRI 4361bp 4360..4360, ori/amp/tet
FT                   -> pKH80 12400bp"
FT   -               1..4359
FT                   /note="pBR322 1..4359 4359bp
FT                   EcoRI = G^AATTC"
FT   -               4360..12635
FT                   /note="pBD9 2..8276..1 8276bp
FT                   EcoRI = G^AATTC"
FT   -               12636..12637
FT                   /note="pBR322 4360..4361 2bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli erythromycin resistance gene (ery)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 12637 BP; 3652 A; 2670 C; 2480 G; 3835 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attccttagt gctttcatag
     attaaactca catcacgctt taaatcgctt attttagact ttaaagactt gttttcttca
     agcaactcat tataatcatt tacattttca ttaaatcgct ctacaagacc actatatttt
     tctttaactt gcccatgttc tttacttaat tttttatatt ctctcgccat atcagtactc
     atgagatttc taacatgctg ttttaaccta tcgttatctc tcgcagcagt cactaagttt
     ttataatcac gctccgatat aacaacattt ttggttggtt tcttttctgt tttcattatt
     tcttttccca aaccaaacat ggacttttca cccgttggca cttcaacact tttcatgtgt
     cgtttcgctg gtacttctaa atctgattta actttatcgc tataagcagt ccattcatct
     tttttaactg ctaaattttt ttctagaaaa tgaatacggt aaggaaaata tgctgtatgc
     gactgtccat ctggatgaaa gagtcccaca tatgcacttt ggttttgtcc ctttaacaga
     ggacgggaga ttgtctgcaa aagaacagtt aggcaacaag aaagacttta ctcaattaca
     agatagattt aatgagtatg tgaatgagaa aggttatgaa cttgaaagag gcacgtccaa
     agaggttaca gaacgagaac ataaagcgat ggatcagtac aagaaagata ctgtatttca
     taaacaggaa ctgcaagaag ttaaggatga gttacagaag gcaaataagc agttacagag
     tggaatagag catatgaggt ctacgaaacc ctttgattat gaaaatgagc gtacaggttt
     gttctctgga cgtgaagaga ctggtagaaa gatattaact gctgatgaat ttgaacgcct
     gcaagaaaca atctcttctg cagaacggat tgttgatgat tacgaaaata ttaagagcac
     agactattac acagaaaatc aagaattaaa aaaacgtaga gagagtttga aagaagtagt
     gaatacatgg aaagaggggt atcacgaaaa aagtaaagag gttaataaat taaagcgaga
     gaatgatagt ttgaatgagc agttgaatgt atcagagaaa tttcaagcta gtacagtgac
     tttatatcgt gctgcgaggg cgaatttccc tgggtttgag aaagggttta ataggcttaa
     agagaaattc tttaatgatt ccaaatttga gcgtgtggga cagtttatgg atgttgtaca
     ggataatgtc cagaaggtcg atagaaagcg tgagaaacag cgtacagacg atttagagat
     gtagaggtac ttttatgccg agaaaacttt ttgcgtgtga cagtccttaa aatatactta
     gagcgtaagc gaaagtagta gcgacagcta ttaactttcg gttgcaaagc tctaggattt
     ttaatggacg cagcgcatca cacgcaaaaa ggaaattgga ataaatgcga aatttgagat
     gttaattaaa gacctttttg aggtcttttt ttcttagatt tttggggtta tttaggggag
     aaaacatagg ggggtactac gacctccccc ctaggtgtcc attgtccatt gtccaaacaa
     ataaataaat attgggtttt taatgttaaa aggttgtttt ttatgttaaa gtgaaaaaaa
     cagatgttgg gaggtacagt gatggttgta gatagaaaag aagagaaaaa agttgctgtt
     actttaagac ttacaacaga agaaaatgag atattaaata gaatcaaaga aaaatataat
     attagcaaat cagatgcaac cggtattcta ataaaaaaat atgcaaagga ggaatacggt
     gcattttaaa caaaaaaaga tagacagcac tggcatgctg cctatctatg actaaatttt
     gttaagtgta ttagcaccgt tattatatca tgagcgaaaa tgtaataaaa gaaactgaaa
     acaagaaaaa ttcaagagga cgtaattgga catttgtttt atatccagaa tcagcaaaag
     ccgagtggtt agagtattta aaagagttac acattcaatt tgtagtgtct ccattacatg
     atagggatac tgatacagaa ggtaggatga aaaaagagca ttatcatatt ctagtgatgt
     atgagggtaa taaatcttat gaacagataa aaataattaa cagaagaatt gaatgcgact
     attccgcaga ttgcaggaag tgtgaaaggt cttgtgagat atatgcttca catggacgat
     cctaataaat ttaaatatca aaaagaagat atgatagttt atggcggtgt agatgttgat
     gaattattaa agaaaacaac aacagataga tataaattaa ttaaagaaat gattgagttt
     attgatgaac aaggaatcgt agaatttaag agtttaatgg attatgcaat gaagtttaaa
     tttgatgatt ggttcccgct tttatgtgat aactcggcgt atgttattca agaatatata
     aaatcaaatc ggtataaatc tgaccgatag attttgaatt taggtgtcac aagacactct
     tttttcgcac cagcgaaaac tggtttaagc cgactgcgca aaagacataa tcgattcaca
     aaaaataggc acacgaaaaa caagttaagg gatgcagttt atgcatccct taacttactt
     attaaataat ttatagctat tgaaaagaga taagaattgt tcaaagctaa tattgtttaa
     atcgtcaatt cctgcatgtt ttaaggaatt gttaaattga ttttttgtaa atattttctt
     gtattctttg ttaacccatt tcataacgaa ataattatac ttttgtttat ctttgtgtga
     tattcttgat ttttttctac ttaatctgat aagtgagcta ttcactttag gtttaggatg
     aaaatattct cttggaacca tacttaatat agaaatatca acttctgcca ttaaaagtaa
     tgccaatgag cgttttgtat ttaataatct tttagcaaac ccgtattcca cgattaaata
     aatctcatta gctatactat caaaaacaat tttgcgtatt atatccgtac ttatgttata
     aggtatatta ccatatattt tataggattg gtttttagga aatttaaact gcaatatatc
     cttgtttaaa acttggaaat tatcgtgatc aacaagttta ttttctgtag ttttgcataa
     tttatggtct atttcaatgg cagttacgaa attacacctc tttactaatt caagggtaaa
     atggcctttt cctgagccga tttcaaagat attatcatgt tcatttaatc ttatatttgt
     cattatttta tctatattat gttttgaagt aataaagttt tgactgtgtt ttatattttt
     ctcgttcatt ataaccctct ttaatttggt tatatgaatt ttgcttatta acgattcatt
     ataaccactt attttttgtt tggttgataa tgaactgtgc tgattacaaa aatactaaaa
     atgcccatat tttttcctcc ttataaaatt agtataatta tagcacgagc tctgataaat
     atgaacatga tgagtgatcg ttaaatttat actgcaatcg gatgcgatta ttgaataaaa
     gatatgagag atttatctaa tttctttttt cttgtaaaaa aagaaagttc ttaaaggttt
     tatagttttg gtcgtagagc acacggttta acgacttaat tacgaagtaa ataagtctag
     tgtgttagac tttatgaaat ctatatacgt ttatatatat ttattatccg gaggtgtagc
     atgtctcatt caattttgag ggttgccaga gttaaaggat caagtaatac aaacgggata
     caaagacata atcaaagaga gaataaaaac tataataata aagacataaa tcatgaggaa
     acatataaaa attatgattt gattaacgca caaaatataa agtataaaga taaaattgat
     gaaacgattg atgagaatta ttcagggaaa cgtaaaattc ggtcagatgc aattcgacat
     gtggacggac tggttacaag tgataaagat ttctttgatg atttaagcgg agaagaaata
     gaacgatttt ttaaagatag cttggagttt ctagaaaatc aatctctttt tccaaagttt
     gttttttaaa tttagctgtc tcaatatgtt tacggtcaga gccacgttca ccacgcttca
     actcaaaacc ctgttttttc atatgctcgg ggaatttatc ttgtagccat aacagttctt
     gacgattaaa cacatttttt ccttgcagtt ttccatcacg cataggcaca acacctaaat
     gcatgtgagg ggtttgctca tcattatgaa ctgttgcata agcaatattt tgcttgccat
     atcgttcgga aaataattta taactttcct caaaaaatcg tttttgttct cctggatcca
     gttgctcaaa aaaatctcgg tcagatgtta ctagcaactc atttacaaga acagcatctt
     tcctccgttt ttcttgtacc tgttttttgt gattcaataa tttctttgac acgttcgttg
     taatcaatat ttttatcatt tttcaaatca taattttcac gtgttcgctc atggtcaata
     tcatcattcg ttctactttt tcgctctctt tgattatgaa attgcatgcc ttttagtcca
     gctgatttca ctttttgcat tctacaaact gcataactca tatgtaaatc gctccttttt
     aggtggcaca aatgtgaggc attttcgctc tttccggcaa ccacttccaa gtaaagtata
     acacactata ctttatattc ataaagtgtg tgctctgcga ggctgtcggc agtgccgacc
     aaaaccataa aacctttaag acctttcttt tttttacgag aaaaaagaaa caaaaaaacc
     tgccctctgc cacctcagca aaggggggtt ttgctctcgt gctcgtttaa aaatcagcaa
     gggacaggta gtattttttg agaagatcac tcaaaaaatc tccaccttta aacccttgcc
     aatttttatt ttgtccgttt tgtctagctt accgaaagcc agactcagca agaataaaat
     ttttattgtc tttcggtttt ctagtgtaac ggacaaaacc actcaaaata aaaaagatac
     aagagaggtc tctcgtatct tttattcagc aatcgcgccc gattgctgaa cagattaata
     atagatttta gctttttatt tgttgaaaaa agctaatcaa attgttgtcg ggatcaatta
     ctgcaaagtc tcgttcatcc caccactgat cttttaatga tgtattgggg tgcaaaatgc
     ccaaaggctt aatatgttga tataattcat caattccctc tacttcaatg cggcaactag
     cagtaccagc aataaacgac tccgcacctg tacaaaccgg tgaatcatta ctacgagagc
     gccagcttca tcacttgcct cccatagatg aatccgaacc tcattacaca ttagaactgc
     gaatccatct tcatggtgaa ccaaagtgaa acctagttta tcgcaataaa aacctatact
     ctttttaata tccccgactg gcaatgccgg gatagactgt aacattctca cgcataaaat
     cccctttcat tttctaatgt aaatctatta ccttattatt aattcaattc gctcataatt
     aatccttttt cttattacgc aaaatggccc gatttaagca caccctttat tccgttaatg
     cgccatgaca gccatgataa ttactaatac taggagaagt taataaatac gtaaccaaca
     tgattaacaa ttattagagg tcatcgttca aaatggtatg cgttttgaca catccactat
     atatccgtgt cgttctgtcc actcctgaat cccattccag aaattctcta gcgattccag
     aagtttctca gagtcggaaa gttgaccaga cattacgaac tggcacagat ggtcataacc
     tgaaggaaga tctgattgct taactgcttc agttaagacc gaagcgctcg tcgtataaca
     gatgcgatga tgcagaccaa tcaacatggc acctgccatt gctacctgta cagtcaagga
     tggtagaaat gttgtcggtc cttgcacacg aatattacgc catttgcctg catattcaaa
     cagctcttct acgataaggg cacaaatcgc atcgtggaac gtttgggctt ctaccgattt
     agcagtttga tacactttct ctaagtatcc acctgaatca taaatcggca aaatagagaa
     aaattgacca tgtgtaagcg gccaatctga ttccacctga gatgcataat ctagtagaat
     ctcttcgcta tcaaaattca cttccacctt ccactcaccg gttgtccatt catggctgaa
     ctctgcttcc tctgttgaca tgacacacat catctcaata tccgaatagg gcccatcagt
     ctgacgacca agagagccat aaacaccaat agccttaaca tcatccccat atttatccaa
     tattcgttcc ttaatttcat gaacaatctt cattctttct tctctagtca ttattattgg
     tccattcact attctcattc ccttttcaga taattttaga tttgcttttc taaataagaa
     tatttggaga gcaccgttct tattcagcta ttaataactc gtcttcctaa gcatccttca
     atccttttaa taacaattat agcatctaat cttcaacaaa ctggcccgtt tgttgaacta
     ctctttaata aaataatttt tccgttccca attccacatt gcaataatag aaaatccatc
     ttcatcggct ttttcgtcat catctgtatg aatcaaatcg ccttcttctg tgtcatcaag
     gtttaatttt ttatgtattt cttttaacaa accaccatag gagattaacc ttttacggtg
     taaaccttcc tccaaatcag acaaacgttt caaattcttt tcttcatcat cggtcataaa
     atccgtatcc tttacaggat attttgcagt ttcgtcaatt gccgattgta tatccgattt
     atatttattt ttcggtcgaa tcatttgaac ttttacattt ggatcatagt ctaatttcat
     tgcctttttc caaaattgaa tccattgttt ttgattcacg tagttttctg tattcttaaa
     ataagttggt tccacacata ccaatacatg catgtgctga ttataagaat tatctttatt
     atttattgtc acttccgttg cacgcataaa accaacaaga tttttattaa tttttttata
     ttgcatcatt cggcgaaatc cttgagccat atctgacaaa ctcttattta attcttcgcc
     atcataaaca tttttaactg ttaatgtgag aaacaaccaa cgaactgttg gcttttgttt
     aataacttca gcaacaacct tttgtgactg aatgccatgt ttcattgctc tcctccagtt
     gcacattgga caaagcctgg atttacaaaa ccacactcga tacaactttc tttcgcctgt
     ttcacgattt tgtttatact ctaatatttc agcacaatct tttactcttt cagccttttt
     aaattcaaga atatgcagaa gttcaaagta atcaacatta gcgattttct tttctctcca
     tggtctcact tttccacttt ttgtcttgtc cactaaaacc cttgattttt catctgaata
     aatgctacta ttaggacaca taatattaaa agaaaccccc atctatttag ttatttgttt
     agtcacttat aactttaaca gatggggttt ttctgtgcaa ccaattttaa gggttttcaa
     tactttaaaa cacatacata ccaacacttc aacgcacctt tcagcaacta aaataaaaat
     gacgttattt ctatatgtat caagataaga aagaacaagt tcaaaaccat caaaaaaaga
     caccttttca ggtgcttttt ttattttata aactcattcc ctgatctcga cttcgttctt
     tttttacctc tcggttatga gttagttcaa attcgttctt tttaggttct aaatcgtgtt
     tttcttggaa ttgtgctgtt ttatccttta ccttgtctac aaacccctta aaaacgtttt
     taaaggcttt taagccgtct gtacgttcct taaggaa
//